We narrowed to 268 results for: aav
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TypeBlog Post...experiment involves delivering a calcium biosensor using AAV, you might decide you’d really prefer to order ready-to-use...promoter for your target cell type Serotype: Choose the AAV capsid or lentiviral prep needed Tags: Identify a...
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Penn Vector Core Partnership with Addgene
TypeCollection...-9-PV2453 45185-AAV9 AAV-EF1a-BbTagBY Control Joshua Sanes AV-9-PV2454 45186-AAV9 AAV-EF1a-BbChT Control...18917-AAV5 AAV-FLEX-rev-ChR2-tdtomato Scott Sternson AV-5-20071P 20071-AAV5 pACAGW-ChR2-Venus-AAV Karel Svoboda...18916P 18916-AAV9 AAV-FLEX-rev-ChR2(H134R)-mCherry Scott Sternson AV-9-18917P 18917-AAV9 AAV-FLEX-rev-ChR2... AV-5-PV2432 22222-AAV5 AAV-FLEX-Arch-GFP Ed Boyden AV-1-ALL864 51503-AAV1 AAV pCAG-FLEX-tdTomato-WPRE...1-27056 27056-AAV1 pAAV-Ef1a-DIO EYFP Control Karl Deisseroth AV-1-ALL854 51502-AAV1 AAV pCAG-FLEX-EGFP-WPRE...9-27056 27056-AAV9 pAAV-Ef1a-DIO EYFP Control Karl Deisseroth AV-9-ALL854 51502-AAV9 AAV pCAG-FLEX-EGFP-WPRE...-ALL854 51502-AAV2 AAV pCAG-FLEX-EGFP-WPRE Control Hongkui Zeng AV-2-PV0101 105530-AAV2 pAAV.CMV.PI.EGFP.WPRE.bGH... -
New CRISPR Tools: Cas7-11 and PASTE
TypeBlog Post...systems have enzymes small enough to fit into a dual AAV system, albeit with lower knockdown efficiencies....HITI and HDR. PASTE is also compatible with both AAV and adenovirus templates. The system, which is larger... -
Hot Plasmids - November 2023
TypeBlog Post... ideal for size-limited AAV delivery. PE6c and PE6d are optimized for dual-AAV systems. These variants... -
Summer Fun at Addgene!
TypeBlog Post...Navigating an Expanding Landscape for Engineered Systemic AAVs” with the director of CalTech’s CLOVER lab, Tim ... -
Editor's Choice, July 2016
TypeBlog Post...Gendered Innovations: Why Does Sex of the Cell Matter? AAV: A Versatile Viral Tool for Gene Expression in Mammals... -
An Interview with Connie Cepko - Gene Therapy, Plasmid Tools and Insights to Success
TypeBlog Post... with Michael Koeris Get Cepko Lab Tips on using AAVs in Your Experiments Resources on the Addgene Website... -
Viral Vectors
TypeCollection... Adeno-associated Virus (AAV) Plasmids | Guide Adeno-associated viruses (AAVs) are commonly used for gene...not permanently integrated into the host genome. AAVs are limited by their small packaging capacity. Adenovirus...research goals . For example, adeno-associated virus (AAV) is typically preferred for in-vivo studies because...Stable RNA <8 Kb 80-130 Dividing/Non-dividing Yes Low AAV Transient or Stable* single stranded linear DNA ∼...<8 Kb 80-130 Dividing Yes Moderate * Recombinant AAV has a low frequency of target cell genome integration... -
Allen Institute for Brain Science AAV Enhancer Collection
TypeCollection...Systemic Capsids AAV Packaged on Request AAV Guide Plasmids Publications Enhancer AAV plasmids are plasmids...Institute for Brain Science AAV Enhancer Collection Allen Institute for Brain Science AAV Enhancer Collection ...Find AAV enhancer plasmids for fluorescent labeling and recombination across brain regions and populations...across brain regions and populations using systemic AAV delivery. The collection includes the top vectors...window) . Figure 1: Selection, prep, and testing of AAV enhancers in mice. Enhancers were selected from the...accessibility across different cell types and cloned into an AAV backbone. Most enhancers were derived from human,...Science Enhancer Collection contains best-in-class AAV plasmids to target a variety of cell populations ... -
Addgene Packaged on Request: Scope of Service
TypeCollection... the AAV1, AAV5, AAV6, AAV8, AAV11, or AAV Retrograde serotypes and 2 x 10 12 GC/mL for the AAV2 serotype...Packaging Service Packaged on Request Scope of Service AAV Packaged on Request: Scope of Service Addgene's Packaged...and limited use label licenses (LULLs). Titer For AAV preps produced through our Packaged on Request service...cases, we may expect lower titers for a particular AAV prep, mostly likely due to one of the following reasons... -
Plan Your Experiment
TypeCollection... collection of in-stock lentiviral preps . AAV vectors AAV vectors offer some advantages for viral vector...delivery. Both lentiviral and AAV vectors are safe to use in the lab, but AAVs exhibits the lowest immunogenicity... transgene expression with AAVs can be long-lived, despite recombinant AAVs not integrating into the host...also frequently use AAVs to develop CRISPR-based therapeutics. One limitation of AAV vectors is that the...blog post on overcoming the AAV size limitation . Browse Addgene's in-stock AAV preps or try Addgene's Packaged...delivery, lentiviral and adeno-associated virus (AAV) vectors are the most common. Using viral vectors...vector) is smaller than that of lentivirus. Thus, AAVs are generally only compatible with smaller Cas enzymes... -
Twenty Years of Sharing: Expanding Accessibility
TypeBlog Post...Views Guides CRISPR Guide Lentiviral Guide AAV Guide Optogenetics Guide γ-Retrovirus Guide ...Plasmids Empty Backbones Bacterial Expression Systems AAV Plasmids Blogs Origin of Replication What... -
Rett Syndrome: A History of Research and Therapeutic Outlooks
TypeBlog Post...the researchers developed adeno-associated viral (AAV) vectors for the targeted delivery of a functional...Syndrome mouse model using an adeno-associated viral (AAV) vector. The editase repaired half of the mutant ... -
An Introduction to Adenovirus
TypeBlog Post...immune response to Adenovirus (and/or to its partner, AAV) or 2) a sociological effect of the COVID-19 lockdowns... (tens of kilobases; compared to only 4.7 kb for AAV, 9 kb for retroviruses). The tradeoff is that adenoviral...(AdV-41), or Adenovirus-Associated Virus type 2 (AAV2) – or some unprecedented combination of the three... -
Chemogenetics Plasmids
TypeCollection...Chemogenetics Guide Viral Service: Chemogenetics AAV Blog: Chemogenetics Viral Vectors 101 Blog: Chemogenetics...collection. For example, search for "hM4D mCherry", "KORD AAV", "LMO", "Sternson", or other terms. Looking for ...for viral preps? We also offer ready-to-use AAV preparations of many chemogenetics plasmids. ID Plasmid ... -
Cre-Lox and Other Site-Specific Recombinases
TypeCollection...Plasmids Pooled Libraries/Kits You may also like… AAV Enhancer Collection Fluorescent Biosensors Chemogenetics...about how to minimize leakiness in experiments using AAV vectors. Highlighted Plasmids Browse the tables below...preps feature Cre-dependent tools, or consider our AAV Packaged on Request service for more options. Addgene...Activity with GFP Plasmids 101: FLEx Vectors Optimizing AAV DIO and FLEx Vector Expression Plasmids 101: Knockout...preps feature Flp-dependent tools, or consider our AAV Packaged on Request service for more options. Addgene...CRISPR Knockout Library CRISPR Sidi Chen Use this AAV CRISPR knockout library expressing luciferase and... -
Tetracycline Inducible Expression
TypeCollection...APEX targeting Alice Ting 85040 pK170.AAV-TRE-Cre-WPRE (Supernova) AAV vector for dox-inducible expression...sgRNA TetR H1-2O2 Nathanael Gray 35625 pAAV-Ptet-RFP-shR-rtTA AAV Tet-On shRNA vector. To evaluate shRNA...promoter Tet-On 3G rtTA David Vereide 120309 pAAV-FAH-rtTA3G AAV vector to express Tet-On 3G transactivator...promoter TetR Eric Campeau , Paul Kaufman 175274 pAAV-rtTA AAV Tet-On vector with neuron-specific expression...Plasmid #27106 for rtTA. tTA Edward Hsiao 99118 pAAV-CAG-tTA AAV Tet-Off vector, expresses tTA from the CAG... Tet-Off tTA-Advanced Takeshi Imai 104109 pAAV-Syn1-tTA AAV Tet-Off vector, expressed tTA from the hSyn...transactivators and inducible tools or consider our AAV Packaged on Request service available for many transactivator... -
Luciferase Plasmid Collection
TypeCollection...Schulze-Lefert 83282 pAAV-CAG-RLuc Renilla CAG AAV expression of Renilla luciferase Mark Kay 83280 pscAAV-CAG-RLuc...Description PI 60226 AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR Renilla EF1α AAV-CRISPR knockout...William Hahn, David Root 105533 pAAV.CMV.Luc.IRES.EGFP.SV40 Firefly CMV AAV expression of firefly luciferase...luciferase expression Stephen Tapscott 83281 pAAV-CAG-FLuc Firefly CAG AAV expression of firefly luciferase Mark...Mark Kay 105532 pAAV.CMV.ffLuciferase.SV40 Firefly CMV AAV expression of firefly luciferase James Wilson...expressing luciferase. We also offer ready-to-use AAV preparations of select luciferase expression plasmids...Renilla CAG Vector for packaging self-complementary AAV expressing Renilla luciferase Mark Kay 140328 pLenti-PGK-Venus-Fluc... -
What's New in CRISPR - September 2019
TypeBlog Post...Tumor Suppressor Gene CRISPR Knockout Library is an AAV CRISPR knockout library targeting mouse tumor suppressor... -
IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging
TypeBlog Post...Immunofluorescence Negative Can Be Positive: Open AAV Data with Addgene Antibodies 101: Choosing the Right...