...Delivery Methods and A Match Made in Heaven: CRISPR and AAV Day 6: Bacteria: CRISPR Methods for Bacterial Genome...

...on the homepage, Blugene is now accompanied by an AAV friend.  We encourage you to take a look and explore...

...Immunofluorescence Negative Can Be Positive: Open AAV Data with Addgene Antibodies 101: Choosing the Right...

...transgenic DNA exceeding the carrying capacity of AAV vectors." Gene Therapy for Neurological Disorders...Hudry, Eloise, and Luk H. Vandenberghe. "Therapeutic AAV gene transfer to the nervous system: a clinical reality...Maguire, 2016). Recombinant adeno-associated viruses (rAAVs) have become increasingly popular for gene delivery...immune responses (Fenno et al., 2011). However, rAAVs lack the ability to specifically infect defined ... promoters, on the other side, are too long for rAAVs, which can only package sequences shorter than 5...vectors in combination with transgenic animals or rAAVs expressing Cre under a specific cell-type promoter... 2019). For example, Taylor and colleagues used rAAV FLEx-based vectors to study the neural circuitry...

...capabilities but are small enough to be packaged in AAV particles. Want more information on the wide variety...

...of GCaMP8 AAV preps has expanded. Check out the new selection of ready-to-use AAV9 and AAVrg vectors. ...available in AAV8! Expand your imaging experiments with the Cre-OFF/FLP-ON vector 137128-AAV8: Ef1a-Coff...

...working with small capacity viral vectors such as AAV. Base removal RNA-guided CRISPR enzymes cause double-stranded...

...Troubleshooting Additional resources on Addgene.org AAV Production in HEK-293T Cells  ...

...crowdsourced Data Hub (built off of the concept of our AAV Data Hub) to share further validation data with you...

...Page Learn How We Use NGS Sequencing to Validate AAV Preps Learn More about Pooled CRISPR Libraries Resources...

...manipulations such as injection of precise titrations of AAV or heat-shocking the organism. The SPARC system uses...

... of a live animal. They started by delivering an AAV construct expressing channelrhodopsin 2 (ChR2), a...

...they drive expression. Different AAV serotypes can be used to target AAV vectors to specific cells of interest...considered. For tissue specificity of AAV serotypes, see our AAV guide . FLEx Vectors are also used to...models. For viral injection, Adeno-associated virus (AAV) is a widely-used tool for achieving in vivo expression...of chemogenetic receptors, and a wide variety of AAV-encoding chemogenetics plasmids are available. Additionally...available. Browse our collection of Ready-to-use AAV Preparations of Chemogenetic Plasmids ! Table 3. ...expression Localization. Depending on the experiment, an AAV-encoded chemogenetic plasmid may need to be targeted...regions of a neuron. Cell-specific expression of AAV-delivered constructs can be controlled with cell-...

...Viral Plasmids Lentivirus Adeno-associated Virus (AAV) γ-Retrovirus Adenovirus All Viral Plasmids Curated...

...and to compare different Cas enzymes in all-in-one AAV delivery. While Fragmid developers were specifically...Fragmid toolkit to facilitate other lentivirus- and AAV-based studies. Unified MoClo Systems While many MoClo...

...elements for deliveries via adeno-associated virus (AAV) and lentivirus, demonstrating simultaneous editing...

...to control the CRISPR system activity.  Using an AAV vector may help with size limitations. In addition...

... Adenoviral Vectors Are adenovirus and AAV different? Yes! AAV is a small single-stranded DNA parvovirus...for replication. For more information about AAV, read our AAV guide . Can I make a stable cell line with...double-stranded DNA viruses. They are not related; however, AAV requires the presence of adenoviral genes E1, E4,...

...off-target effects, yet small enough to be packaged in AAV. A dCasMini-mediated adenine base editor allows for...

...plasmids alone. (And we expect that resources like our AAV Data Hub will help scientists save even more!) If...