...them easy to harvest from cell culture media. Their His tags allow for further purification, if desired, ...targeted the following antigens: GFP, RFP,  the histone protein H2A2B, and the actin-binding protein gelsolin...however, compatible with most multi-labeling immunohistochemical protocols where either a directly labeled...RANbodies) as versatile, small, sensitive immunohistochemical reagents. PNAS. PubMed PMID: 29440485. Additional...

...this cloning technique! Many thanks to Rob Hurt for his help on this piece. Rob is postdoc in Peter Schultz...

...paper to understand what Shoukhrat Mitalipov and his colleagues have achieved, and how the field will ...

...speech recognition, he returned to school to merge his interests in biology and computing. In 2009, he founded...

...this if he wants data by next week!”, and “This is his job!” filled my naive mind. Moving forward from that...

...John Doench reports even the greenest members of his lab, high school interns, were able to use Fragmid...

...-lytic insect cell expression systems (sf9, Sf21, Hi-5 cells)  that allow for continuous expression of...

...) from the two yeast species into the pYES2.1/V5-His yeast overexpression vector. This plasmid contains...promoter. The cloning procedure adds both V5 and His6 epitope tags to the C-termini of Met proteins, which...

...I've attempted to answer several emails that start: “Hi! We ordered something from you and it’s wrong. Can...

... can be found climbing mountains or playing with his two cats. Keira Havens grew up in Hawaii where she...

... poster a few years ago at a CRISPR meeting.Read his Instagram feature here. You also don’t have to...

... at the Biohub in San Francisco. Before starting his career in software, he completed a MSc in Psychology...HDR. Optimal mutation in-depth One the most sophisticated features of CrispyCrunch is how it mutates guide...

... who later shared a Nobel Prize in Chemistry for his work. Since then PCR has become a standard and essential...

...al., 2023). Our thanks to Rodolphe Barrangou for his authorial contributions to, and support for, this...

...developed and demonstrated by Manuel Gonçalves and his colleagues at Leiden University Medical Center are...

...our guest blogger, Jon Chow! Jon Chow is finishing his immunology PhD at Harvard University. He has had ...

...Centre for Regenerative Medicine (CRM), Edinburgh. His research interests include optimizing genome-editing...

...Haimovich of greenfluorescentblog. Gal will share his list of 10 things to keep in mind when choosing the...

... so as to benefit all people and the planet. For his many efforts in open science, Endy was recognized...

...created the pMAK backbone by modifying pMAL-T-Avi-His/BirA, a vector commonly used for in vivo biotinylation... localization signal or including fusions with Histone 2A or 2B can result in bright targeted expression... desired nanobody (NB) sequence, an AviTAG, and 6xHis tag. The MBP signal peptide allows the NB to be ...