Addgene: The First Twenty Years
Type
Blog Post
...Béthune, Professor at HAW Hamburg, posting about his Blue Flame Award on Bluesky.
In 2016, we also began...
We narrowed to 412 results for: his
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Human Germline Editing Using CRISPR
TypeBlog Post...paper to understand what Shoukhrat Mitalipov and his colleagues have achieved, and how the field will ... -
Experimenting in an Open Source lab: from CRISPR to Cats
TypeBlog Post...speech recognition, he returned to school to merge his interests in biology and computing. In 2009, he founded... -
5 Steps to Writing Grant Proposals for Grad Students
TypeBlog Post...this if he wants data by next week!”, and “This is his job!” filled my naive mind. Moving forward from that... -
Plasmids 101: Protein Expression
TypeBlog Post...-lytic insect cell expression systems (sf9, Sf21, Hi-5 cells) that allow for continuous expression of... -
Pathways Over Time Plasmids Engage Students in Functional Genomics Research
TypeBlog Post...) from the two yeast species into the pYES2.1/V5-His yeast overexpression vector. This plasmid contains...promoter. The cloning procedure adds both V5 and His6 epitope tags to the C-termini of Met proteins, which... -
Making CRISPR Plasmids Using Fragmid
TypeBlog Post...John Doench reports even the greenest members of his lab, high school interns, were able to use Fragmid... -
Tips for Technical Support Calls
TypeBlog Post...I've attempted to answer several emails that start: “Hi! We ordered something from you and it’s wrong. Can... -
Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)
TypeBlog Post... can be found climbing mountains or playing with his two cats. Keira Havens grew up in Hawaii where she... -
Antibody Guide
TypeGuide..., or by using a secondary against a tag, such as His, incorporated in the sdAb. sdAbs can have weak signals...location. Includes: Immunofluorescence (IF) Immunohistochemistry (IHC) Immunocytochemistry (ICC) Cell Sorting...in these assays. This method is suitable for DNA:histone modifier interactions, which have strong binding...methods such as immunofluorescence (IF), immunohistochemistry (IHC), and immunocytochemistry (ICC) use... they only have one protein of interest. Immunohistochemistry (IHC) and immunocytochemistry (ICC) IF can... can refer to both immunohistochemistry and immunocytochemistry when fluorescent signaling molecules (... -
Polymerase Chain Reaction Overview and Applications
TypeBlog Post... who later shared a Nobel Prize in Chemistry for his work. Since then PCR has become a standard and essential... -
Meet an Addgenie: Insights into Grad School, Career Paths, and More
TypeBlog Post... poster a few years ago at a CRISPR meeting.Read his Instagram feature here. You also don’t have to... -
CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments
TypeBlog Post... at the Biohub in San Francisco. Before starting his career in software, he completed a MSc in Psychology...HDR. Optimal mutation in-depth One the most sophisticated features of CrispyCrunch is how it mutates guide... -
Quick Guide to Working with Drosophila Part 1: Getting Started with Flies
TypeBlog Post...our guest blogger, Jon Chow! Jon Chow is finishing his immunology PhD at Harvard University. He has had ... -
Twenty Years of Addgene Sharing: CRISPR
TypeBlog Post...al., 2023). Our thanks to Rodolphe Barrangou for his authorial contributions to, and support for, this... -
Google Forums Round Up: First Impressions of NgAgo
TypeBlog Post...Centre for Regenerative Medicine (CRM), Edinburgh. His research interests include optimizing genome-editing... -
Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells
TypeBlog Post...developed and demonstrated by Manuel Gonçalves and his colleagues at Leiden University Medical Center are... -
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)
TypeBlog Post...Haimovich of greenfluorescentblog. Gal will share his list of 10 things to keep in mind when choosing the... -
Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection
TypeBlog Post... so as to benefit all people and the planet. For his many efforts in open science, Endy was recognized... -
Hot Plasmids - August 2020
TypeBlog Post...created the pMAK backbone by modifying pMAL-T-Avi-His/BirA, a vector commonly used for in vivo biotinylation... localization signal or including fusions with Histone 2A or 2B can result in bright targeted expression... desired nanobody (NB) sequence, an AviTAG, and 6xHis tag. The MBP signal peptide allows the NB to be ...