We narrowed to 412 results for: his

1. Plasmids 101: Modular Cloning

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   Blog Post

   ...this cloning technique! Many thanks to Rob Hurt for his help on this piece. Rob is postdoc in Peter Schultz...

2. Experimenting in an Open Source lab: from CRISPR to Cats

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   ...speech recognition, he returned to school to merge his interests in biology and computing. In 2009, he founded...

3. 5 Steps to Writing Grant Proposals for Grad Students

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   ...this if he wants data by next week!”, and “This is his job!” filled my naive mind. Moving forward from that...

4. Making CRISPR Plasmids Using Fragmid

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   ...John Doench reports even the greenest members of his lab, high school interns, were able to use Fragmid...

5. Plasmids 101: Protein Expression

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   ...-lytic insect cell expression systems (sf9, Sf21, Hi-5 cells)  that allow for continuous expression of...

6. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

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   ...) from the two yeast species into the pYES2.1/V5-His yeast overexpression vector. This plasmid contains...promoter. The cloning procedure adds both V5 and His6 epitope tags to the C-termini of Met proteins, which...

7. Tips for Technical Support Calls

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   ...I've attempted to answer several emails that start: “Hi! We ordered something from you and it’s wrong. Can...

8. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

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   ... can be found climbing mountains or playing with his two cats. Keira Havens grew up in Hawaii where she...

9. Antibody Guide

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   Guide

   ..., or by using a secondary against a tag, such as His, incorporated in the sdAb. sdAbs can have weak signals...location. Includes: Immunofluorescence (IF) Immunohistochemistry (IHC) Immunocytochemistry (ICC) Cell Sorting...in these assays. This method is suitable for DNA:histone modifier interactions, which have strong binding...methods such as immunofluorescence (IF), immunohistochemistry (IHC), and immunocytochemistry (ICC) use... they only have one protein of interest. Immunohistochemistry (IHC) and immunocytochemistry (ICC) IF can... can refer to both immunohistochemistry and immunocytochemistry when fluorescent signaling molecules (...

10. Twenty Years of Addgene Sharing: CRISPR

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    ...al., 2023). Our thanks to Rodolphe Barrangou for his authorial contributions to, and support for, this...

11. Meet an Addgenie: Insights into Grad School, Career Paths, and More

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    ... poster a few years ago at a CRISPR meeting.Read his Instagram feature here. You also don’t have to...

12. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

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    ...our guest blogger, Jon Chow! Jon Chow is finishing his immunology PhD at Harvard University. He has had ...

13. Polymerase Chain Reaction Overview and Applications

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    ... who later shared a Nobel Prize in Chemistry for his work. Since then PCR has become a standard and essential...

14. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

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    ... at the Biohub in San Francisco. Before starting his career in software, he completed a MSc in Psychology...HDR. Optimal mutation in-depth One the most sophisticated features of CrispyCrunch is how it mutates guide...

15. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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    ...developed and demonstrated by Manuel Gonçalves and his colleagues at Leiden University Medical Center are...

16. Google Forums Round Up: First Impressions of NgAgo

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    ...Centre for Regenerative Medicine (CRM), Edinburgh. His research interests include optimizing genome-editing...

17. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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    ...Haimovich of greenfluorescentblog. Gal will share his list of 10 things to keep in mind when choosing the...

18. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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    ... so as to benefit all people and the planet. For his many efforts in open science, Endy was recognized...

19. Hot Plasmids - August 2020

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    ...created the pMAK backbone by modifying pMAL-T-Avi-His/BirA, a vector commonly used for in vivo biotinylation... localization signal or including fusions with Histone 2A or 2B can result in bright targeted expression... desired nanobody (NB) sequence, an AviTAG, and 6xHis tag. The MBP signal peptide allows the NB to be ...

20. Lab to Office Culture Shock

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    ...Big thank you to Jessica Welch, Mike Lemieux (for his advice and for modeling in the picture above), and...