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We narrowed to 876 results for: lic

Showing: 161 - 180 of 876 results

  1. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...Require Sialic Acid Binding for Hemagglutination and Efficient Transduction but Differ in Sialic Acid Linkage...receptor Coreceptor AAV1 α2,3-/α2,6 N-linked sialic acids1 AAV receptor (AAVR)2   AAV2 Heparan...Laminin receptor (LR)3 CD93 AAV5 2,3-N-linked sialic acid4 AAV receptor (AAVR)2 Platelet-derived...(PDGFR) α and β3 AAV6 α2,3-/α2,6 N-linked sialic acids1 Heparan sulfate proteoglycan (HSPG)3 AAV...vessel barriers. It is especially popular for applications targeting liver tissue. In addition, it has ...the blood-brain barrier, making it useful for applications targeting the CNS (Issa et al., 2023). Additionally...treatment, which can pose problems for gene therapy applications. Cross-reactivity of AAV serotypes varies by...

  2. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Activate Catalytically dead dCas9 fused to a ...specific locus. ID Plasmid Gene/Insert Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression...

  3. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ...Purchase and the applicable material transfer agreements (MTAs) and limited use label licenses (LULLs). Titer...contact you directly regarding these charges, if applicable....

  4. Hot Plasmids: Summer 2025

    Type
    Blog Post
    ...between mGold2s and mGold2t depending on the application. Figure 1: Super-resolution imaging...from Lee & Lai et al. (2025) under CC BY-NC-ND license. If you’re currently using mVenus or mCitrine...proof-of-concept, they believe that the strategy will be applicable with other TnpBs and in other plant species, ...available for tracking cell lineage, different applications require careful selection or design to ensure...Reproduced from Singh et al. (2025) under CC BY license. Libraries are available with T-Sapphire, ...for gene therapy and ex vivo cell engineering applications and is especially well-suited for lung cells...tool for circuit tracing and other neuroscience applications (Figure 4). Check out our blog post on AAV serotypes...

  5. CRISPR Antimicrobials

    Type
    Blog Post
    ...suppliers, and public health and medical organizations are working to help educate the public about proper...containing a phage origin of replication that can be packaged into replication-incompetent phage particles...Beisel et al., licensed under a Creative Commons Attribution 4.0 International License.   Improving...essential processes: cell wall synthesis, DNA replication, or protein synthesis. Because they target such...mammalian viral vectors in that the vectors are replication-deficient. Thus, a very large number of phagemids...CRISPR antimicrobials combines them with both replication-competent phages and antibiotics. Phage therapy...bacterial host range. To further potential clinical applications, Ando et al. recently engineered synthetic phages...

  6. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    ...for both transient and stable gene expression applications in plants. This smaller, easily customizable...Agrobacterium are termed binary vectors because they must replicate in two species: Agrobacterium and E. coli. Like.... coli. However, they also need to be able to replicate in Agrobacterium to ensure that they are maintained... Thus, binary vectors contain two origins of replication, one each for E. coli and Agrobacterium, along...bases each. By switching the E. coli origin of replication and codon-optimizing the Agrobacterium ORI, they...constitutively active M24 promoter has been modified with duplicated enhancer domains and characterized in studies...these properties of pSiM24 are suitable for many applications, the plasmid can also be customized due to its...

  7. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...to disparate physiological effects and complicate any metabolic study. The Ralser lab has created a unique.... Seeliger MA, Young M, Henderso MN, Pellicena P, King DS, Falick AM, and Kuriyan J. Protein Sci. 2005...brief articles highlight the main features and applications of a partiular plasmid or set of plasmids. We...Wu Lab library can be found in the associated publication. Ma, et al. Cell Rep. 2015. PubMed PMID: 26190106...and ubiquitous reporter assay. In their recent publication, Kroeze et al. describe their enhanced TANGO .... Each gene was chosen to represent the primary splice variant in 38 types of human cancer. Many of the...Sanger sequencing, respectively. The speed and simplicity of GMAP allows scientists to easily design, generate...

  8. AAV for Neuronal Tracing

    Type
    Collection
    ...which is not required for the transcription or replication of the genome within infected cells, but is required... cells that lack the G protein, the virus can replicate but the newly formed viruses are unable to infect...starter cell via the EnvA receptor. RABV is able to replicate in the starter cell using the G-protein, which...

  9. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...emission wavelengths, which are pivotal in their application in biotechnology (Grimm & Lavis, 2022). These... substrates/peptides and antibodies. Example applications of this include coupling to an antibody, whereby...typically bind to. Stability of fluorescence: For applications that involve ranges of pH, and significant lengths...light, is another factor to consider. If your application requires longer periods of exposure, you’ll want...have shown that different fluorescent ligands can elicit varying degrees of β-arrestin-2 recruitment to ...descriptions, or technical manuals. Specific applications and matching dye/ligand choices For effective...beyond the diffraction limit of light. Dyes like silicon-rhodamine or Janelia Fluor (JF) dyes have specific...

  10. Hot Plasmids - November 2023

    Type
    Blog Post
    ...for your application. Image reused form Doman and Pandey et al., 2023 under CC-BY 4.0 License. Find... CreVLPs, and eVLPs for in vivo and in vitro applications.   Figure 2: Modular features of DIRECTED...Strebinger et al., 2023, reused under CC-BY 4.0 License. Importantly, even commercial antibodies can...approach for experimental therapies and medical applications.  Find DIRECTED plasmids here! Strebinger, D...interactions or map a local proteome, but some applications have been challenging or inaccessible. The Ting...alkyne-phenol, to label proteins with an alkyne group for click chemistry reactions instead of biotin — a valuable...from Lin et al., 2023 bioRxiv, under CC-BY-NC 4.0 license. This system provides a unique new way to record...

  11. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

    Type
    Blog Post
    ...list of individual screens curated for the publication. Clicking on the screen name takes users to a display...favorite gene was described in a CRISPR screen publication. You see this mentioned in the results section...manually curated and annotated from more than 45 publications, beginning with the seminal research articles... makes it possible for researchers to query all public CRISPR results for any gene of interest. In addition...screen curated in ORCS is linked to the original publication via its PubMed identifier, which can also be ...screens of interest.   Figure 3: Each publication featured in BioGRID ORCS has a dedicated summary... supplemental data tables available with the publication are also provided for the user. This approach...

  12. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ...Minimized amount of silica ACT environmental impact factor label Non-silica based, avoids use of... plasmid preps are the same. Your downstream application will dictate how much DNA you need to recover...recover and the level of quality required. Some applications require very little DNA and you can get away with...with slightly lower quality preps, while other applications require large amounts of high quality DNA. Figure... a High Quantity/Low Quality mindset for any applications. DNA quantity is fairly self explanatory and... In a column-based approach, the substrate is a silica matrix fixed in a column and you flow lysate and...settings, but when it comes to low-throughput applications, the choice is largely a matter of preference...

  13. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ...Depending on the research question and downstream application, one or several of those techniques can be used...your flow analysis. However, certain downstream applications, like an in vivo adoptive transfer of those ...available fix/perm kits allow for labeling of cytosolic proteins, as well as proteins of the secretory...intranuclear proteins of interest, you can label cytosolic proteins alongside the intranuclear ones. You ...You do not need to use a separate fix/perm cytosolic procedure in that case.     Figure 3: For intracellular...or free amines of proteins. Depending on the application, they can be membrane-permeant or membrane-impermeant...are positive or negative for the dye. The main applications of dye stains in flow cytometry are cell proliferation...

  14. Viral Vector Guides and Plasmids

    Type
    Collection
    ...strong immunogenic response, useful for clinical applications as vaccines. Selecting a Viral Vector Type Each...been carefully engineered for safety and are replication-incompetent. Specific genes have been mutated...mutated or deleted to prevent uncontrolled viral replication, and the required genes are divided in the multi-plasmid...

  15. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    ...what we do not understand, and the complicated and unique metabolic processes of plants are being investigated...involved in plant synthetic biology. Challenge #1: Public perception of synthetic biology One of the biggest...difficulties that plant biotechnologists face is public perception of genetic modification (GM) and synthetic...benefits and future potential of the technology to the public in an open, responsible manner. At Revolution Bio...obstacles to plant synthetic biology In addition to public resistance, an overall limited understanding of...knockouts to better understand developmental, metabolic, and photosynthetic processes. Often the tools... intellectual property concerns – patents and licensing agreements. Developing a product without the use...

  16. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...constitutive splicing studies and has been optimized for high efficiency splicing. If intron splicing does not...potential applications of CRISPR/Cas9 in genome-engineering. One particularly attractive application involves...in order to avoid complicating effects from global toxicity due to loss of splicing. To this end, destabilizing... system widely accessible for many potential applications. Polstein LR & Gersbach CA, Nat Chem Biol ...amenable to both high and low throughput screening applications. The system can be readily modified to use different...cloning platform for imaging and flow cytometry applications Designing customized fluorescent reporters can...luciferase reporter system for detection of pre-mRNA splicing   The Gideon Dreyfuss lab has developed rapid-response...

  17. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ...between bacteria can also have serious implications for public health. Bacteriocin plasmids: microbial...us! A plasmid is a small piece of DNA that can replicate independently from its host’s chromosome. Although...Some bacteria possess plasmid-borne toxins like colicins and subtilisins to kill other bacteria in the ...plasmids carrying genes for bacterial toxins like colicin typically also carry the necessary resistance genes...survive their own secretions. Pro tip! Historically, colicin-producing plasmids have been referred to as “Col...inducible expression systems that allow tuning of metabolic pathways. Fertility (F) plasmids: microbial sharing... common plasmid features, such as origins of replication and promoters, check out our other Plasmids 101...

  18. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...host genome, the virus is replication incompetent, meaning it can’t replicate and continue the infection... tools, thought was given on how to keep the replication of virus and its infectivity in check. Engineering...envelope plasmid), and the polymerase (Pol) for replication and Gag structural protein on yet a third plasmid... this safety feature prevents the virus from replicating and spreading in non-target cells, including ....    Viral cargo-specific safety   After replication competency, the second concern with virus safety... be disposed of following your lab’s biohazard policies. Administering Virus Many virus experiments involve... accident-prone researchers. Note that safety policies will vary slightly from organization to organization...

  19. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...most transposable elements (TEs) results in the duplication of this target site sequence at the site of insertion...This is similar to how retroviruses, like HIV, replicate. Class I TEs do not encode a transposase enzyme...enzyme.  Class I TEs are considered replicative since every time they jump they make a copy of themselves...retroviruses both in their structure and mechanism of replication. They contain two genes: gag and pol. The pol...they move. Most Class II transposons have a non-replicative “cut and paste” mechanism of transposition: they... it integrates, which produces a target site duplication (TSD). Created in BioRender.com.   Autonomous...transposition activity  Tol2 transposon plasmids    Applications of transposons Now that you’ve learned about...

  20. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...Institute for Cell Science has produced the first publicly available collection (Link opens in a new window...Proliferating cell nuclear antigen (PCNA) DNA replication foci IPSC cell lines can be ordered from the ...: 28814507 (Link opens in a new window) More publications from the Allen Institute for Cell Science (Link...
Showing: 161 - 180 of 876 results