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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 210 results for: pcr

Showing: 161 - 180 of 210 results

  1. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...minutes at 95°C in a PCR machine or in a beaker of boiling water. If using a PCR machine, incubate the...Days post-infection mRNA knockdown (quantitative PCR) ≥3 days Protein knockdown (western blot) ≥4 days...

  2. Plan Your Experiment

    Type
    Guide
    ...methods like sequencing or digital PCR , RNA-based methods like qPCR , and/or protein-based methods like...Very inefficient Can't distinguish specific edits PCR Knockouts Large insertions/deletions Some prime and...provide specific sequence information alone Digital PCR Knockouts Insertions/deletions Prime and base edits...RNA-sequencing, Perturb-seq, deep sequencing, etc. qPCR Knockouts Activation/interference Needs good primer...

  4. Guide to Using Pooled Libraries

    Type
    Guide
    ...surviving cells are collected, their plasmids are PCR-amplified and sequenced using NGS to identify their...

  5. Molecular Biology Reference

    Type
    Guide
    ...single-stranded DNA sequence used as an initiation point for PCR amplification or DNA sequencing of the plasmid. Primers...

  6. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...controlling GPCR activation have been developed to selectively study individual types of GPCRs with greater...be used for lower frequency stimulation. GPCR signaling GPCR signaling is a complicated process involving...change the membrane potential. OptoXRs are modified GPCRs. When exposed to light, they activate downstream... coupled receptors  G-protein coupled receptors (GPCR) regulate cellular function through multiple intra-cellular...have been developed for utilizing light to control GPCR activation and downstream signaling cascades (reviewed...Levitz, 2020), including light-sensitive chimeric GPCRS (OptoXRs) (Tichy et al., 2019), photoswitchable ...rhodopsin, and the intracellular domain of an endogenous GPCR, such as adrenergic receptors. When exposed to light...

  7. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...requires ddPCR skills. Your resume lists ddPCR, so the hiring manager knows that you have ddPCR experience...with ddPCR, but do have experience with qPCR, you could talk about how your experience with qPCR would...that by describing how you developed and used a ddPCR assay to answer an experimental question. Now, the...manager knows that you can design experiments with ddPCR, interpret the results, and develop a successful...would make it easy to master ddPCR.  Finally, it’s a good idea to state why you’re applying to the specific...

  8. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...biosensors kit By Mike Lacy G protein-coupled receptors (GPCRs) are critical components of many physiological pathways...targets. To enable large-scale profiling of many GPCRs, Mikel Garcia-Marcos’ lab recently developed a collection... detection of activation triggered by endogenous GPCR. Example applications include large-scale pharmacological...The authors used this platform to assay dozens of GPCRs across many cell types in varying conditions, revealing...2024). Direct interrogation of context-dependent GPCR activity with a universal biosensor platform. Cell...

  9. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...titer results between ddPCR and qPCR? A: At CLOVER, we have traditionally used qPCR (protocol link). We ...this to strongly bias titer. We are also adopting ddPCR, which does not a standard curve and somewhat more...

  10. Addgene Protocols Go Viral: AAV Purification and Titration

    Type
    Blog Post
    ...titration by qPCR using SYBR green technology Here’s some advice on how to successfully set up your qPCR to determine...generate a standard curve, visit the AAV Titration by qPCR written protocol page. Getting started with tissue...

  11. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...clinical and diagnostic labs, deep sequencing and qPCR methods are too costly and time intensive to process...platform on clinical stool samples by comparison to qPCR and demonstrated that it can also detect expression...mRNA expression cannot be identified with DNA-based qPCR methods, the ability to detect mRNA transcripts ...

  12. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    ...signaling pathways G protein-coupled receptors (GPCRs) detect molecules outside of the cell and translate...those signals into cellular responses. For example, GPCRs and their downstream G protein partners can regulate...have a long standing interest in the biology of GPCRs and G proteins, with an emphasis on oligomerization...

  13. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...designer drugs, are G protein-coupled receptors (GPCRs), that have been reprogrammed to respond only to... threshold and silence neurons. DREADD-activated GPCR signaling can also modify other properties such ...

  15. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ... loop regions of any G-protein-coupled receptor (GPCR) recognizing a specific ligand (Ino et al. 2022)...produce more than 20 other MTRIA sensors based on GPCRs recognizing various neurotransmitters, neuropeptides...

  16. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...promoters (orange). Hybridoma-derived V L and V H domain PCR products are fused to a joining fragment comprising...translocation of the heavy chain across the ER membrane. PCR-mediated fusion of these three elements is followed...

  17. Immunology Research Plasmids and Resources

    Type
    Collection
    ...PROCR protein C receptor, endothelial (EPCR) CCCA, CCD41, CD201, EPCR, MGC23024, bA42O4.2 PSMB8 proteasome...receptor 2 CYSLT2, CYSLT2R, GPCR, HG57, HPN321, KPG_011, PSEC0146, hGPCR21 DARC Duffy blood group, chemokine...relaxin/insulin-like family peptide receptor 3 GPCR135, MGC141998, MGC142000, RLN3R1, RXFPR3, SALPR SAA1... estrogen receptor 1 CEPR, CMKRL2, DRY12, FEG-1, GPCR-Br, GPR30, LERGU, LERGU2, LyGPR, MGC99678 GPHA2 ...repeat-containing G protein-coupled receptor 6 FLJ14471, GPCR, VTS20631 LHB luteinizing hormone beta polypeptide...relaxin/insulin-like family peptide receptor 3 GPCR135, MGC141998, MGC142000, RLN3R1, RXFPR3, SALPR RXRA...

  18. AAV Packaged on Request is Here!

    Type
    Blog Post
    ...quality control processes as our in-stock service: ddPCR, purity testing, and sterility testing, plus viral...

  19. New Viral Vectors - Summer 2024

    Type
    Blog Post
    ...new optogenetic tool called PdCO.  This is an "optoGPCR" (coupled to Go, so it's an inhibitory opsin!)...

  20. Brain Initiative Collection

    Type
    Collection
    .... Used for photoswitchable control of inhibitory GPCR signaling cascades. Michael Bruchas 139505-AAV8 .... Used for photoswitchable control of inhibitory GPCR signaling cascades. Michael Bruchas 139505-AAV9 .... Used for photoswitchable control of inhibitory GPCR signaling cascades. Michael Bruchas 140135-AAV1 ...
Showing: 161 - 180 of 210 results