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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 171 results for: plasmid for e coli

Showing: 161 - 171 of 171 results

  1. Sequencing Primers

    Type
    Guide
    ... For sequencing plasmids in our repository, we've chosen primers based on the plasmid backbone and insert...ATGCCATAGCATTTTTATCC (Invitrogen) For vectors with E. coli araBAD promoter, forward primer pBAD Reverse GATTTAATCTGTATCAGG...GATTTAATCTGTATCAGG (Invitrogen) For vectors with E. coli araBAD promoter, reverse primer pBluescriptKS TCGAGGTCGACGGTATC...uses for sequence verification of deposited plasmids. Plasmid... Plasmid Reference Molecular Biology Reference Sequencing... for sanger sequence verification of deposited plasmids. Below is a list of commonly used primers. This...GCAACGTGCTGGTTATTGTG Rabbit beta-globin intron, for pCAG plasmids, forward primer pCasper-F GGGTTTTATTAACTTACAT ...

  2. Adenovirus Guide

    Type
    Guide
    ...you generally need two plasmids: Shuttle plasmid (also known as transfer plasmid) — containing the transgene...shuttle plasmid. For a summary of all adenoviral plasmid components, see the Adenoviral Plasmid Elements...are BJ5183 E. coli cells containing pAdEasy. pAdEasy is a ~33 kb adenoviral backbone plasmid containing...AdEasier-1 cells BJ5183 E. coli cells containing the pAdEasy-1 packaging plasmid. Adenovirus (AdV) Wildtype...for recombination with the adenoviral plasmid. Adenoviral plasmid — containing the rest of the adenoviral...chosen plasmid is compatible with cloning methods available in your lab. When cloning your plasmids, be ...consists of two plasmids that eventually recombine to form one: Shuttle/transfer plasmid (e.g. pAdTrack...

  3. CRISPR Guide

    Type
    Guide
    ...36593413 Vo, P. L. H., Ronda, C., Klompe, S. E., Chen, E. E., Acree, C., Wang, H. H., & Sternberg, S. H... R., Hanna, R. E., Hegde, M., Donovan, K. F., Strand, C., Sullender, M. E., Vaimberg, E. W., Goodale, ... the DNA and replaces it with another base. In E. coli , this strategy yields C to A base edits, whereas...harnessed for antiviral mechanisms in bacteria ( E. coli ). Like the other Cas enzymes, the Cascade-Cas3...edits. PASSIGE ( P rime- E diting- A ssisted S ite- S pecific I ntegrase G ene E diting), developed by the.... PMID: 26735016 Lee, J. K., Jeong, E., Lee, J., Jung, M., Shin, E., Kim, Y., Lee, K., Jung, I., Kim, ..., K. M., Aach, J., Guell, M., DiCarlo, J. E., Norville, J. E., & Church, G. M. (2013). RNA-Guided Human...

  4. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ... on the plasmid. gRNA plasmids that do not co-express a Cas protein require a separate plasmid that does...gRNA Vectors CRISPR Plasmids: Empty gRNA Expression Vectors Browse CRISPR Plasmids By Function Genome ... these independent gRNA plasmids can be paired with a wide variety of plasmids and therefore are not limited.... Whether the plasmid contains Cas9 and if so, which function of Cas9. We have plasmids with Cas9 that...none, need plasmid 65775 N. meningitidis Joung VVT1 65779 Mammalian BsmBI none, need plasmid 65776 S. aureus...expression of Csy4 (eg Plasmid 53369 ). Joung pX333 Mammalian Single all-in-one plasmid for tandem expression...types of plasmids available for use in this Golden Gate and Gateway based assembly method: plasmids for assembly...

  5. Promoters

    Type
    Guide
    ...biology basics and plasmid components. Plasmids 101: The Promoter Region-Let's Go Plasmids 101: Inducible ... popular Plasmids 101 series , covers topics ranging from the newest breakthroughs in plasmid technologies...Inducible Promoters Plasmids 101: Repressible Promoters Plasmids 101: Terminators and PolyA signals...operon trp Repressible by tryptophan Promoter from E. coli tryptophan operon Ptac Regulated like the lac promoter... Plasmid Reference Molecular Biology Reference Promoters...

  6. Cloning

    Type
    Guide
    ... of interest (YGOI) into a recipient plasmid. (Image from Plasmid 101: Restriction cloning ) Back to Top...product into a plasmid. The major disadvantage of TOPO® cloning is that very few plasmid backbones are ...Entry plasmids are also available, allowing for rapid cloning of PCR products into donor plasmids without.... The product may be transformed directly into E. coli , where the nicks will be repaired by the normal...manipulate genes of interest and then insert them into plasmids for replication and protein expression. So how...fragments. Given its prevalence, the vast majority of plasmids used for DNA cloning and expression contain several...restriction sites on either side of it into any plasmid that has the same sites in the same orientation...

  7. Mammalian RNAi Tools

    Type
    Collection
    ... Plasmid Collections Mammalian RNAi Mammalian shRNA Tools for RNAi Browse Plasmids Resources ...BioRender.com. shRNA Plasmids Browse our selection of empty vectors for cloning in shRNAs. Most plasmids are in a ...Plasmids for mammalian RNA interference (RNAi) experiments at Addgene; browse by constitutive lentiviral... occur via transfection or viral delivery of a plasmid. shRNAs are often delivered using viral vectors...with some retroviral and AAV backbone options. Plasmids for constitutive expression as well as those that...inducible (Tet) expression are available. To find plasmids containing other RNAi components, like argonaute...argonaute, please search our full site . ID Plasmid Vector Type PI Publication Additional Resources Addgene...

  8. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail help...Addgene Blog: Biosensors Luciferase Plasmids Optogenetics Plasmids Viral Service: AAV Biosensors Fluorescent...Osmolarity SED1 osmolarity biosensor, for expression in E. coli , yeast, or plants Intrinsically disordered protein...Find plasmids encoding fluorescent biosensors to measure biomolecules or genes via FRET or other assays... Plasmid Collections Fluorescent Proteins Fluorescent Biosensors Fluorescent Proteins: Biosensors...our catalog . Use the article links to find the plasmids associated with the article. We also offer ready-to-use...ready-to-use AAV preparations of select plasmids and AAV Packaged on Request for many more. Metal Ions ...

  9. TALEN Plasmids and Kits

    Type
    Collection
    ...for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail help...List of plasmid kits and accessory plasmids available from Addgene for generating your own specific TALE...Yamamoto Additional Plasmids for Use with Addgene TALEN Kits For Use With: ID Plasmid Depositing PI Description...TALE Toolbox 49622 - 49647 24 plasmids Thoru Pederson TALEColor plasmids: destination vectors for use ... TALE plasmids. Genome... Genome Engineering TALENs TALEN Plasmids and Kits You may also like... Addgene's Genome...Genome Engineering Guide CRISPR Plasmids Zinc Finger Kits TALENs are important new tools for genome engineering...

  10. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...NeuroMab/Trimmer Lab Plasmid Collection R-mAb Plasmids scFv Plasmids Nanobody Plasmids Protocols References... Antibody Plasmid Collection NeuroMab Plasmid Collection... the plasmid, followed by the SV40 polyA tail (SV40 pA). Recombinant Monoclonal Antibody Plasmid Collection...following plasmids can be used to produce R-mAbs from transfected mammalian cells. Addgene ID Plasmid Target...partner labs. Learn More scFv Plasmid Collection The following plasmids can be used to produce scFvs from... Pan-GRK Mouse Mouse Nanobody Plasmid Collection The following plasmids can be used to produce nanobodies...Anti-MBP [N128A/4R] Maltose binding protein (MBP) E. coli Mouse IgG2a 206532 Anti-Keyhole limpet hemocyanin...

  11. Plasmids for Stem Cell Research

    Type
    Collection
    ... Plasmid Collections Plasmids for Stem Cell Research Plasmids for Stem Cell Research...Reprogramming Plasmids Differentiation and Transdifferentiation Plasmids Stem Cell Research Plasmids Stem cells...for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail help...utilize plasmids to increase expression of key factors. The tables below will help you find plasmids available...information page. Reprogramming Plasmids Browse the table below for plasmids used to create induced pluripotent...RNA p53 for single plasmid reprogramming Novel codon-optimized mini-intronic plasmid for efficient, inexpensive...Differentiation and Transdifferentiation Plasmids Browse the table below for plasmids used for differentiation of ...
Showing: 161 - 171 of 171 results