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We narrowed to 963 results for: App;

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  6. AAV Production in HEK293 Cells

    Type
    Protocol
    ...incubator for ~72 h. Harvest cells and media by tapping the sides of the CS5. Cells should detach easily...

  7. Antibody Guide

    Type
    Guide
    ...single assay. Overview of Antibody Applications Antibody-based applications can be generally classed into ...these applications, visit the Antibody section of the Addgene Protocols page. Antibody Applications - Quantification...of signaling molecules. Select one appropriate for your application and experimental design. Will you ...transcriptomics or proteomics. This approach is often appropriate for quantitative methods. Capture MS...protein of interest, with no overlapping epitopes. It is not appropriate for validation of an antibody...production and storage techniques, and explain common applications. Science... Handling Visualization Signal Amplification Application Overview Quantification Methods Capture Methods...

  8. CRISPR Guide

    Type
    Guide
    ... (2015). A scalable Genome-Editing-Based approach for mapping multiprotein complexes in human cells. Cell...technologies. Before CRISPR, genome engineering approaches like zinc finger nucleases (ZFNs) or transcription-activator-like...rapidly became the most popular genome engineering approach. Figure 1: Overview of the basic CRISPR mechanism... to various Cas enzymes have extended CRISPR applications to increasingly complex functions, including...understanding of CRISPR biology, introduce the various applications of CRISPR, and help you get started using CRISPR... Cas12a, which are available for specialized applications. Check out our list of additional Cas proteins...genomic edits carried out by Cas9. Multiplexing applications include editing multiple genes at once; using...

  9. Guide to Using Pooled Libraries

    Type
    Guide
    ...library. Apply pooled library to cells. DIVERGE: Positive and negative screens POSITIVE screen: Apply library...control sample (no selection) In parallel, apply library Apply selection; most cells will survive Perform..., semi-random sequences that can be used for applications like lineage tracing or parsing the effects ...library Apply selection Most cells die or do not pass selection (in the case of a reporter) Sort “winning...genes involved in the selection NEGATIVE screen: Apply library Perform Next Generation Sequencing (NGS)...and control cells Generate a list of gRNAs that disappear with addition of the selection mechanism Positive...

  10. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...to be used as viral vectors for gene therapy applications. This guide contains many viral vector-specific...preferred over standard rAAV vectors for in vivo applications. Other Engineered Variants Hybrid capsids Hybrid...Vectors rAAV vectors are valuable in diverse applications due to their unique properties, including their...biomedical fields. Gene Therapy One of the major applications of rAAV vectors is in the field of gene therapy...mutagenesis. Several AAV-based gene therapies have been approved by the US Food and Drug Administration (FDA) to...neurons within the brain. Large-scale examples of mapping the brain through neuronal tracing with rAAV vectors...transgenes, enabling the development of further AAV applications. Read our blog post on split AAVs for more information...
Showing: 941 - 950 of 963 results