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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 963 results for: App;

Showing: 951 - 960 of 963 results

  1. Plan Your Experiment

    Type
    Guide
    ...as an example, but many of these principles also apply to using CRISPR in other organisms. As with most...component for each category. Genetic Manipulation Application Cas Enzyme Additional Considerations Browse Plasmids... Cas enzymes increase specificity Dual-nickase approach increases specificity but is less efficient Each...gene you target will depend on your specific application. Knockout : Target constitutively expressed regions...gRNA, you can design a donor DNA to have the appropriate left and right homology arms. See Addgene's HDR...transient or stable). For plasmid and viral-based approaches, the species and expression pattern of the promoter...cationic lipids, electroporation, or an endosomal approach. Similar to the mRNA method described above, this...

  2. Cloning

    Type
    Guide
    ...move around pieces of DNA. Oftentimes several approaches will work for any specific cloning project; however...likely that for any given project there is an ideal approach. This may be due to speed, cost, availability ... of time required for cloning. To utilize this approach, the fragment of DNA that you would like to clone...fragment (PCR product or synthesized oligo) with appropriate overhangs can be efficiently ligated into any... enzyme cloning. Scientists have utilized this approach to create compatible custom overhangs that can...fragments of dsDNA that have 30 or more bases of overlapping homology. One major advantage is that much larger...

  3. Modular Cloning Guide

    Type
    Guide
    ...Modular Cloning Blog: Plasmids 101 Modular Cloning Applications and Kits Blog: Plasmids 101 Golden Gate Cloning... between. MoClo can be used for a variety of applications—like building synthetic genetic circuits, engineering...proteins for use in E. coli for a variety of applications. EcoFlex MoClo Extension, Volume I Bacterial... vectors for use in E. coli for a variety of applications. CIDAR MoClo Parts Kit Bacterial Expression ...CRISPR vectors compatible with various delivery approaches, enzymes, and mechanisms. zCas9i cloning kit:... genome editing and transcription modulation applications Golden Gate TALEN and TAL Effector Kit 2.0 Genome...

  4. Gamma-Retroviral Vector Guide

    Type
    Guide
    ... popular choice in gene therapy applications. There are many approved gamma-retroviral gene therapies ...retroviral vectors are commonly used in gene therapy applications to more specifically direct delivery of gene...lentiviral vectors, but a similar method can be applied when using gamma-retroviral vectors. Gene Therapy...cell (CAR-T) therapy is a particularly popular application of gamma-retroviral vectors. Gamma-retroviral...Gammaretroviral vectors: Biology, technology and application. Viruses , 3 (6), 677–713. https://doi.org/10.3390...

  5. Lentiviral Vector Guide

    Type
    Guide
    ...random and difficult to predict. In therapeutic applications, like chimeric antigen receptor T cell (CAR-...Pseudotyped lentiviral vectors are common in clinical applications, like gene therapy, as you can more specifically...accurate and multiplexable method for genome-wide mapping of lentiviral integration sites. Molecular Therapy... in chromatin architecture through large-scale mapping of lentiviral integration sites. Nature Protocols...2020). An analytical pipeline for identifying and mapping the integration sites of HIV and other retroviruses...begins, by definition, at the beginning of R, is capped, and proceeds through U5 and the rest of the provirus...

  6. Chemogenetics Guide

    Type
    Guide
    ...neurons) that can be turned on or off by the application of a small molecule ligand. The ideal chemogenetic...translational studies because it is FDA and EMA approved. One pitfall of a suite of muscarinic-based DREADDs... develop a PSAM that responded to a clinically approved drug, the PSAM domain was engineered to respond...Azua IR, Li JH, et al. (2009). A chemical-genetic approach to study G protein regulation of β cell function...chemogenetics for research and potential clinical applications. Science. 364(6436):eaav5282. PMID: 30872534...

  7. Optogenetics Guide

    Type
    Guide
    ...the light source. Optogenetics has been broadly applied to study the physiology of the brain and nervous... peak expression of the opsin is reached. This approach is especially powerful when combined with site...conformation/dimerization. In addition to the applications described above, both LOV and Dronpa can control...KR, Deisseroth K. 2010 Molecular and cellular approaches for diversifying and extending optogenetics. ..., Yizhar O, Deisseroth K. 2011. Principles for applying optogenetic tools derived from direct comparative...

  8. Molecular Biology Reference

    Type
    Guide
    ...species and can be useful for a diverse set of applications - Plasmids can drive gene expression in a wide...a primer. A primer is a small piece of DNA, approximately 18-22 nucleotides, that binds to complementary...each fluorescent signal. Sanger can sequence approximately 500-1000 bases downstream of the known primer...pieces in only a few reactions.There are multiple approaches to acquire NGS but one of the most commonly used...

  9. Addgene's eBook Collection

    Type
    Guide
    ...contains an introduction to each vector and its applications, tips for using vectors in your experiments,...your transferable skills, and tips on the job application process. Close About Overlay Select CRISPR 101...introductions and helpful tips for antibody-based applications, from flow to IHC! Close About Overlay 0 of ...

  10. Adenovirus Guide

    Type
    Guide
    ..., which has limited their use in therapeutic applications. However, this has also made them the most widely...Adenovirus-mediated gene delivery: potential applications for gene and cell-based therapies in the new...
Showing: 951 - 960 of 963 results