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Showing: 41 - 60 of 142 results
  1. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...Derived Transcriptional Activator Systems. PLoS ONE 11:e0158294 . https://doi.org/10.1371/journal.pone.0158294...control of endogenous gene activation. Nat Chem Biol 11:198–200 . https://doi.org/10.1038/nchembio.1753 Tanenbaum...
  2. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ... National Academy of Sciences 110.18 (2013). 7306-11. Pubmed PMID: 23589850. 9. Cronin, J., Zhang, X.Y...PMID: 22407723. PubMed Central PMCID: PMC3456965. 11. Zhang B., Metharom P., Jullie H., Ellem K.A., Cleghorn...
  3. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    ...from researchers at Amgen, it was stated that only 11% of the academic research in the literature was reproducible...specific compliance requirements – e.g. 21 CFR part 11, HIPAA? Answering this question helps you evaluate...
  4. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...using CRISPR. Biochem Biophys Res Commun. 2013 Aug 11. pii: S0006-291X(13)01329-6. doi: 10.1016/j.bbrc.2013.08.013...
  5. Zhang Lab CRISPR Page

    Type
    Collection
    ...Agarwala V, Scott DA, Zhang F. Nat Protoc . 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013...Sanjana NE, Shalem O, Zhang F. Nat Methods . 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047. PubMed . Return...
  6. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ... for multicolor imaging across scales. Nat Commun 11, 239 (2020). Oliinyk, O.S., Shemetov, A.A., Pletnev...dynamics of protein-protein interactions. Nat Methods 11, 641-644 (2014). Filonov, G.S. & Verkhusha, V.V. ...
  7. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ... in human somatic cells. Nature Protocols 2007; 2(11): 2734-46. PubMed PMID: 18007609. Addgene Materials...Sanjana NE, Shalem O, Zhang F. Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047. 10.1038/nmeth.3047...
  8. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...Polstein LR & Gersbach CA, Nat Chem Biol 2015 Mar;11(3):198-200. SunTag system for single molecule imaging... Synthetic Biology Center have deposited a set of 11 phage integrases which act irreversibly on their ...basis for Gateway cloning. The combination of all 11 att pairs in series with unique spacer sequences ...pMemoryArray) gives a DNA sequence capable of recording 11 bits (1.375 bytes) of information. This leads to ...pMemoryArray.   Yang et al., Nat Methods 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Bioluminescent... Schmidl, et al. ACS Synth Biol. 2014 Nov 21;3(11):820-31. Inncredible new tools for epitope tagging...
  9. Important Considerations When Using AAVs

    Type
    Blog Post
    ...of AAVs as a new tool for neuroanatomical tracing (11). With the ability to produce neuron specific transfection...therapy 18.2 (2007): 171-182. PubMed PMID: 17328683. 11. Wouterlood, Floris G., et al. "A fourth generation...
  10. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...agarose gel electrophoresis. Methods Mol Biol. 2009;582:11-25. doi: 10.1007/978-1-60761-340-4_2. PMID: 19763938...concatemers in mammalian cells. J Gene Med. 2009 May;11(5):444-53. doi: 10.1002/jgm.1310. PMID: 19263463....
  11. CRISPR Guide

    Type
    Collection
    ..., Alshareef S, Mahfouz MM. Biochem J . Jun 11;475(11):1955-1964. PMID: 29891532 CRISPR-Based Technologies...Grunwald D, Zhang S, Pederson T.. Nat Methods . Nov;15(11):928-931. PMID: 30377374 CtIP fusion to Cas9 enhances...Correia BE, Eils R, Niopek D. Nat Methods . Nov;15(11):924-927. PMID: 30377362 Engineered CRISPR-Cas9 nuclease...Becavin C, Depardieu F, Bikard D. PLoS Genet . Nov 7;14(11):e1007749. PMID: 30403660 Optimized libraries for... J, Backofen R, Koonin EV. Nat Rev Microbiol . 13(11):722-36. PMID: 26411297 Cationic lipid-mediated delivery... J, Agarwala V, Scott DA, Zhang F. Nat Protoc . 8(11):2281-308. PMID: 24157548 High-frequency off-target...
  12. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...Proceedings of the National Academy of Sciences USA 112(11) (2015): 3570-5. PubMed PMID: 25733849. PubMed Central...PMC4115528. Find plasmids from this paper at Addgene. 11. Cress, Brady F., et al. “CRISPathBrick: Modular ...
Showing: 41 - 60 of 142 results