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We narrowed to 16 results for: Agt

Showing: 1 - 16 of 16 results

  1. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...fusion tag based on the human DNA repair protein AGT that gets covalently linked to derivatives of its...Also in 2008, Kai Johnsson’s lab reported another AGT-based tag named CLIP-tag, which reacts with O2-benzylcytosine...benzylguanine (BG) (Keppler et al., 2003). This engineered hAGT later became known as SNAP-tag. There is also a ...

  2. Plasmid Cloning by PCR

    Type
    Blog Post
    ...Forward Primer will use the sequence 5'-ATGTGGCATATCTCGAAGTAC-3' for the region that binds the ORF and...primer, making our Forward Primer 5'-GAATTCATGTGGCATATCTCGAAGTAC-3'. Many restriction enzymes do not cut...final Forward Primer sequence of 5'-TAAGCAGAATTCATGTGGCATATCTCGAAGTAC-3'. For the Reverse Primer, the design...of the ORF, including the stop codon (5'-TGGCATATCTCGAAGTACTGA-3'), then adding NotI (GCGGCCGC) and then.... This gives us a sequence of 5'-TGGCATATCTCGAAGTACTGAGCGGCCGCTAAGCA-3' (30bp with 18bp of homology to...chose for our reverse primer (5’-TGGCATATCTCGAAGTACTGAGCGGCCGCTAAGCA-3’) into this calculator we get a...final Reverse Primer sequence of 5’-TGCTTAGCGGCCGCTCAGTACTTCGAGATATGCCA-3’. Preparing the PCR product for...

  3. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...canonical loxP sequence is ATAACTTCGTATA-GCATACAT-TATACGAAGTTAT. The loxP sequence does not occur naturally ...

  4. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ... asun TAL3404 & TAL3405 TAACTCAAGCCAACTCCAggaaacatcaggagtttGCAGGAAGATTGAGTTCA atf6 TAL3406 & TAL3407 TCCAACCAGCAAAAAGACccatccaggtgtgccccaAGGTTTCCATTCAGCCTA...TCCAACCAGCAAAAAGACccatccaggtgtgccccaAGGTTTCCATTCAGCCTA atg5 TAL3218 & TAL3219 TAAAGTCAAGAAACATTTtctcaaagtcatgaagGCAGAGGATGTGGAGGAA atg12 TAL3216 & TAL3217 ...TAL3217 TTAATCAGTCGTTTGCGCcatcaccggaccaagaAGTTGGTGTGCTTTTTGA avpl TAL3220 & TAL3221 TGCTGTGTGTGCTCGCGCtctccacgctctcgtcTGCCTGCTACATCCAGAA...TCAGCTCCCCGGAGAGGCaccgcactgctccagggTTGGATGATGGACATGGA bsx TAL3034 & TAL3035 TCTCAGACTCACAGTTATccggactcgagaaaagGTTCGAGATCCAGAGATA btr30 TAL3036 & TAL3037 ...TATAACAAATCCCACCACattgcccgatatcttgAATACTTCCAAAACAAAA Ccl2 TAL3222 & TAL3223 TTTTCGTTTTCCACTATAaagatcccagttaagcAGGTTCAGAGTTATCATA cebpa TAL3040 & TAL3041 ... cebpb TAL3224 & TAL3225 TGGAAGTGGCCGGTTTTTacgagggggactacctCGCTTTCCACAGCACTAA cebpd TAL3042 & TAL3043 ...TAL3043 TGACTTCTGCTGCGTCCAtgtctgacatgtacaatCTGGACTCGCAGTGCGTA CHCHD4 TAL3408 & TAL3409 TCTACCTAATGGCGACATaaactggaactgcccgtGTTTGGGCGGCATGGCCA...

  5. Validated gRNA Sequences

    Type
    Collection
    ...sapiens GACTGTAAGTGAATTACTT 69539 cut S. pyogenes 26130722 Yan HPRT1 H. sapiens CTGATAAAATCTACAGTCAT 58778...synthetic GCGTATAGTACACATTATACG 66585 cut S. pyogenes 26018130 Xue LRTM2 H. sapiens GCTGGCGGAAGACAGAGTGC 69237...musculus GCTGTAGTAATATCTGCTAT 66588 cut S. pyogenes 26018130 Xue Pten M. musculus GTTTCATAGCGGCCACGAAGT 66587...elegans GGAAGGACATAGTTGTCAT 59935 cut S. pyogenes 25161212 Fire sqt-1 C. elegans TGTGGAGTTGGGGTAGCGT 60212...sapiens GGTGAGTGAGTGTGTGCGTG 69235 cut S. pyogenes 26480473 Wolfe TS4 H. sapiens GAGTCCGAGCAGAAGAAGAA 69236...elegans ATATCAGTCTGTTTCGTAA 47550 cut S. pyogenes 23995389 Goldstein tyr D. rerio CCCCAGAAGTCCTCCAGTCC 46761...sapiens GGGTGGGGGGAGTTTGCTCC 47505 cut S. pyogenes 23792628 Joung VEGF H. sapiens GGTGAGTGAGTGTGTGCGTG 47507...

  6. Zinc Finger Consortium: Zinc Finger Arrays

    Type
    Collection
    ...CODA CODA aGTCCTCCGATGGCCCAGGTGATGTTc SDC3 OZ595 and OZ596 CODA CODA aGTCCGCCTCCACCCGAGGTAGAG zgc:111821...gCGTGACCTCgctgatAAGAAGAGg xylt1 OZ509 and OZ510 OPEN OPEN cACCCTCTACaggacaGTAGCGGGTg zgc:153086 OZ511 and OZ512 OPEN OPEN ...aGACTCCGTCccgtcgtGCTGAGGGCc hnf1a OZ521 and OZ522 OPEN OPEN aACCTTCCCCagagaGAGGTAGTGg VDAC2 OZ523 and OZ524 OPEN OPEN gCTCCCAACCactttGCTGGTGTCa...lipoprotein receptor) OZ539 and OZ540 OPEN OPEN tTGCATCCACggcagTCGGCAGTGc Encephalopsin (Opn3) OZ541 and OZ542 OPEN...aTCCATCCCAaacacatGTGGCAGAAt NFATc1 OZ543 and OZ544 OPEN OPEN aCCTCGCCTCagtgtGACGGAGGAc Swachmann-Bodian-Diamond Syndrome Gene...tTACTTCTGCaatgatGTGGTGGCTc vipr1 OZ559 and OZ560 OPEN OPEN gGCCATCAGCtcgcattGGAGAAGTAg NRF2b (nfe212b) OZ561 and OZ562 OPEN ...tGTCTTCATCTCCTTCAGATGCTGTCc fam82a2 OZ573 and OZ574 CODA CODA gCACCGCTGCAGTAAGAGCTGAGGAAt morc3b (NP_001003579.1) OZ575 and OZ576...

  7. Synthetic Biology - Assembly Standards Guide

    Type
    Collection
    ...) Prefix Enzymes: EcoR1 , NotI , XbaI Suffix: T ACTAGT A GCGGCCG CTGCAG Suffix Enzymes: SpeI , NotI , ...BioBrick BB-2 (RFC 12) Prefix: GAATTC GCGGCCGC T ACTAGT G Prefix Enzymes: EcoR1 , NotI , Spel Suffix: GCTAGC...CTGCAG Suffix Enzymes: Nhel , NotI , PstI Scar: GCTAGT Features: 6 bp scar encodes Ala-Ser; Allows for...G Prefix Enzymes: EcoR1 , NotI , XbaI Suffix: T ACTAGT A GCGGCCG CTGCAG Suffix Enzymes: SpeI , NotI , ...EcoR1 , NotI , XbaI, (NgoMIV) Suffix: ACCGGT TAAT ACTAGT A GCGGCCG CTGCAG Suffix Enzymes: Agel , Spel , ...

  8. Immunology Research Plasmids and Resources

    Type
    Collection
    ...protein homolog (mouse) AGRT, ART, ASIP2, MGC118963 AGT angiotensinogen (serpin peptidase inhibitor, clade...FLJ92595, FLJ97926, SERPINA8 AGTR1 angiotensin II receptor, type 1 AG2S, AGTR1A, AGTR1B, AT1, AT1B, AT1R, AT2R1...AT2R1, AT2R1A, AT2R1B, HAT1R AGTR2 angiotensin II receptor, type 2 AT2, ATGR2, MRX88 AMBN ameloblastin ...dJ647M16.1 APLN apelin XNPEP2 APLNR apelin receptor AGTRL1, APJ, APJR, FLJ90771, MGC45246 AR androgen receptor...

  9. Sequencing Primers

    Type
    Guide
    ...primer Luc-F AGTCAAGTAACAACCGCGA 3' end of luciferase, forward primer LucNrev CCTTATGCAGTTGCTCTCC 5' end of...primer M13 (-21) Forward TGTAAAACGACGGCCAGT In lacZ gene M13 (-40) GTTTTCCCAGTCACGAC In lacZ gene M13 Reverse...Human U6 promoter, forward primer LucNrev CCTTATGCAGTTGCTCTCC 5' end of luciferase, reverse primer M13 ...AUG1 promoter, forward primer AUG1 Reverse GAAGAGAAAAACATTAGTTGGC (Invitrogen) For Pichia vectors with AUG1...AUG1 promoter, reverse primer BGH Reverse TAGAAGGCACAGTCGAGG (Invitrogen) Bovine growth hormone terminator...terminator, reverse primer Ecdysone Forward CTCTGAATACTTTCAACAAGTTAC (Invitrogen) Drosophila heat shock promoter...promoter, forward primer EF-1a Forward TCAAGCCTCAGACAGTGGTTC (Invitrogen) Human elongation factor-1a promoter...

  10. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...Forward Primer will use the sequence 5'-ATGTGGCATATCTCGAAGTAC-3' for the region that binds the ORF and...primer, making our Forward Primer 5'-GAATTCATGTGGCATATCTCGAAGTAC-3'. Many restriction enzymes do not cut...final Forward Primer sequence of 5'-TAAGCAGAATTCATGTGGCATATCTCGAAGTAC-3'. For the Reverse Primer, the design...of the ORF, including the stop codon (5'-TGGCATATCTCGAAGTACTGA-3'), then adding NotI (GCGGCCGC) and then.... This gives us a sequence of 5'-TGGCATATCTCGAAGTACTGAGCGGCCGCTAAGCA-3' (30bp with 18bp of homology to...chose for our reverse primer (5’-TGGCATATCTCGAAGTACTGAGCGGCCGCTAAGCA-3’) into this calculator we get a...final Reverse Primer sequence of 5’-TGCTTAGCGGCCGCTCAGTACTTCGAGATATGCCA-3’. Experimental Procedure Run PCR...

  13. AAV ddPCR Titration

    Type
    Protocol
    ... Forward Primer: 5’-CGGCCTCAGTGAGCGA ITR Reverse Primer: 5’-GGAACCCCTAGTGATGGAGTT ITR Probe: -FAM-CACTCCCTCTCTGCGCGCTCG-BBQ...

  16. Lentivirus ddPCR Titration

    Type
    Protocol
    ...Primers/probe targeting RRE: forward primer: tgtgccttggaatgctagt probe (FAM): tttggaatcacacgacct reverse primer...
Showing: 1 - 16 of 16 results