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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 11 results for: Dnmt3a

Showing: 1 - 11 of 11 results

  1. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...Methyltransferase 3 Alpha (DNMT3A) Vlatka Zoldoš’ lab has deposited pdCas9-DNMT3A-EGFP and pdCas9-DNMT3A-PuroR for targeted...For lentiviral expression, Fuw-dCas9-Dnmt3a and Fuw-dCas9-Dnmt3a-P2A-tagBFP are available from Rudolf ...ready-to-use lentivirus. Figure 3: dCas9-DNMT3A methylates cytosines at promoters and enhancers...transient and quickly reversed in culture. However, DNMT3A-induced methylation persisted throughout a 100 ...

  4. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ...histone demethylation by LSD1 cytosine methylation by DNMT3A or MQ1 cytosine demethylation by Tet1 These modifications...

  5. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...the endogenous, autoinhibited methyltransferase DNMT3A to the genomic location specified by the DNA-binding...

  6. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ... pdCas9-DNMT3A-EGFP 71666 Mammalian U6 yes, methylation S. pyogenes EGFP Zoldos pdCas9-DNMT3A-PuroR 71667...Mammalian S. pyogenes Neo, mCherry Postovit pdCas9-DNMT3A-PuroR_v2 74407 Mammalian U6 yes, methylation S....

  8. Validated gRNA Sequences

    Type
    Collection
    ...Sabatini DNMT3A H. sapiens GAGATGATCGCCCCTTCTTC 41822 cut S. pyogenes 23287722 Church DNMT3A H. sapiens...

  9. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...the catalytic domain from DNA methyltransferase, DNMT3A. Co-expression of this modified Cas9 along with...

  10. CRISPR Guide

    Type
    Guide
    ...TET1 - activation through cytosine demethylation DNMT3A - repression through cytosine methylation MQ1 -...labs , is an all-in-one dCas9 fusion with KRAB, DNMT3A, and DNMT3L. CRISPRoff maintains gene silencing...

  11. CRISPR Guide

    Type
    Collection
    ...TET1 - activation through cytosine demethylation DNMT3A - repression through cytosine methylation MQ1 -...labs , is an all-in-one dCas9 fusion with KRAB, DNMT3A, and DNMT3L. CRISPRoff maintains gene silencing...
Showing: 1 - 11 of 11 results