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Addgene
Showing: 1 - 20 of 221 results
  1. Plasmids for Stem Cell Research

    Type
    Collection
    ...transgene-free induced pluripotent stem cells. Proc Natl Acad Sci U S A. 2014 Jul 22;111(29):10678-83. Capecchi...cells using a single polycistronic vector. Proc Natl Acad Sci U S A. 2009 Jan 6. 106(1):157-62. Jaenisch Lentivirus...modulation of canonical Wnt signaling. Proc Natl Acad Sci U S A. 2012 May 29. Palecek Fibroblasts Cardiomyocytes...human fibroblasts to dopaminergic neurons. Proc Natl Acad Sci U S A. 2011 Jun 21;108(25):10343-8. Parmar Fibroblasts...self-renewing, tripotent neural precursor cells. Proc Natl Acad Sci U S A. 2012 Feb 14;109(7):2527-32. Wernig Fibroblasts...
  2. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    ...career outside the traditional academic silo to tenured professor.  Non-academia encompasses millions of choices...designate non-academic jobs as “alternative” anymore.   Since most scientists start out in academia, it is...and perspectives to consider. What do academic and non-academic careers in science have in common? You...but there is more flexibility with the academic calendar Academic researchers must be extreme self-starters...Biomedical workforce will NOT go into “traditional” academic positions. More recently, I have been hearing ...careers as their training progresses.  In From Academic Solos to Industrial Symphonies, an article well...jump into industry.” In his article On Leaving Academe, Terran Lane is asked by his colleagues and friends...
  3. Chemogenetics Guide

    Type
    Guide
    ...potently activated by an inert ligand. Proc. Natl. Acad. Sci. USA 104:5163–68. PMID: 17360345 Chen X, Choo...specifically designed Gi-coupled receptor. Proc. Natl. Acad. Sci. USA 95:352–57. PMID: 9419379 Davies MA, Compton-Toth...regulation of β cell function in vivo. Proc. Natl. Acad. Sci. USA 106:19197–202. PMID: 19858481 Magnus CJ...
  4. Cre-lox system

    Type
    Collection
    ...Site-Specific Recombination in Transgenic Mice. Proc Natl Acad Sci. Jul 15;89(14):6232-6. PubMed . Matsuda T, Cepko...Introduced by In Vivo Electroporation. Proc Natl Acad Sci. Jan 16;104(3):1027-32. doi: 10.1073/pnas.0610155104...the Cre Recombinase of Bacteriophage P1. Proc Natl Acad Sci. Jul;85(14):5166-70. PubMed . Sternberg N, Hamilton...Conditional RNA Interference from Transgenes. Proc Natl Acad Sci. Jul 13;101(28):10380-5. doi:10.1073/pnas.0403954101...
  5. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...genetically encoded fluorescent sensor. Proc Natl Acad Sci U S A. 2004 Dec 14;101(50):17404-9. Ca2+ indicators...Zn2+ with genetically encoded sensors. Proc Natl Acad Sci U S A. 2011 May 3;108(18):7351-6. Amy Palmer...revealed by single GFP-linked biosensors. Proc Natl Acad Sci U S A. 2008 Jan 8. Wolfgang Dostmann cGMP (cyclic...release at Schaffer collateral synapses. Proc Natl Acad Sci U S A. 2018 May 7. pii: 1720648115. Katalin ...and neurons of Caenorhabditis elegans. Proc Natl Acad Sci U S A. 2019 Aug 1. Alexander Gottschalk Voltage...
  6. Academic vs. Industry Postdocs

    Type
    Blog Post
    ... industry and academia. Pros and cons: The academic vs. industry postdoc  Time: Academic postdocs can ...still incredibly nervous about leaving academia. A lot of academics tend to view industry as “the dark side...your goal is to enter academia, it is easier to make that transition from an academic setting—especially ..., an academic advisor could also lose their funding. Communication: In both industry and academia, you...AstraZeneca’s Waltham site after doing a brief academic postdoc. Why did I switch? Well, my long-term ...acquire. Learning about drug discovery: Unless your academic lab is closely involved with clinical trials or... be a bit of a mystery to graduate students in academia. Getting a drug to the market usually takes over...
  7. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    ....   Advice for success in academia How can we shape our time in academia to maximize productivity and...who's seen every spurious result under the sun. Academia is about convincing results, and nothing is more...pragmatic.” Advice for pursuing careers outside of academia Recently, graduate students and postdocs have...have become more aware of careers outside of academia. How do PIs assist their mentees who have goals outside...recommends that a student interested in leaving academia should present their research at conferences to... applicable to a variety of careers outside of academia. She has had previous graduate students and postdocs... success in academia...
  8. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    ... When transitioning from an academic science career path to a non-academic one, one of the biggest changes...Curricula Vitae to showcase your publications, academic performances, and research experiences, you now...consider when you're preparing your resume for a non-academic position: What is a resume? How is it different.... If you are still debating whether to stay in academia or not, it is even more important to start early... about your plan for the next 5 years. Does an academic career work well with your life and career plan...emphasis for graduate student resumes UC Irvine Non Academic Job Search Handbook & Resumes  Read Our Blog Post...
  9. Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry

    Type
    Blog Post
    ... became available: distribution was limited to academic scientists. Expanding plasmid sharing during ...plasmids available more broadly: to scientists in academia and industry. As a result over 90% of Addgene’...                                       Chinese Academy of Medical Sciences University of Texas at Austin...these plasmids to the scientific community - both academic and industry scientists. This partnership complemented...organizations have made their plasmids available beyond academia. For example: Plasmids from the Michael J. Fox...scientists To make plasmid sharing more open for academic and industry scientists, we’ve worked with many...
  10. Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene

    Type
    Blog Post
    ...outside of academia. These roles can include positions, like mine, that help support academia but the skills... did I join Addgene? Was I jaded by my time in academia? Did I run screaming from the lab like the cartoon...dramatic story, but it wouldn’t be my story. I love academics. If I didn’t, it would have been unwise of me ...pretend that that there aren’t problems with the academic enterprise or that there weren’t some pretty hefty...are led to believe that they can all end up in academic positions (I’ve even blogged about this here and... my story demonstrates that transitioning from academia doesn’t have to mean abandoning it or running ...more glad that I thought about careers outside of academia during my graduate training. PhDs have many roles...
  11. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

    Type
    Blog Post
    ...magnetic cancer cell capture. Fierer et al (Proc Natl Acad Sci U S A. 2014 Mar 17. PubMed) Peptide tag forming...engineering a bacterial adhesin. Zakeri et al (Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):E690-7. Epub 2012...study published in Proceedings of the National Academy of Sciences in March, he and his colleagues took...time? Howarth: The Proceedings of the National Academy of Sciences paper [published online on March 17...
  12. The Pleiades Promoter Project

    Type
    Collection
    ...drive selective expression in the brain. Proc Natl Acad Sci U S A . 2010 Sep 21;107(38):16589-94. PubMed...
  13. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ...Ligand-Regulated Site-Specific Recombination.” Proc. Natl. Acad. Sci. USA 92(June): 5940–44. PubMed PMID: 7597057...Ligand-Dependent Chimeric Cre Recombinase.” Proc Natl Acad Sci U S A 92(15): 6991–95. PubMed PMID: 7624356....
  14. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ... by tetracycline-responsive promoters. Proc Natl Acad Sci U S A. 1992 Jun 15;89(12):5547-51. doi: 10.1073...derived from the trp and lac promoters.” Proc Natl Acad Sci U S A. 80(1) (1983): 21-5. PubMed PMID: 6337371...
  15. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...transposase tools for genome engineering.,” Proc. Natl. Acad. Sci. U. S. A., vol. 110, no. 25, pp. E2279–87, ...tool for conditional cell ablation.,” Proc. Natl. Acad. Sci. U. S. A., vol. 105, no. 38, pp. 14492–6, Sep...
  16. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...Escherichia coli is sequence specific."  Proc Natl Acad Sci U S A.  1991 Nov 15;88(22):10104-8. PubMed PMID...
Showing: 1 - 20 of 221 results