...To use this tool to detect a BRAF gene edit, simply PCR-amplify the BRAF locus from the edited cells (...product should contain the edited BRAF sequence), and PCR-amplify the BRAF locus from control cells (the ... the 428 bp band indicates edits in the BRAF gene in the BRAF-gRNA transduced cells, and no such edits.../Cas9 gene edit. I wanted to inactivate the gene BRAF (a kinase implicated in several human cancers) in...editing, don’t expect a 100% knockout population. BRAF gRNA delivery  Overview: In practice you would select...chose one of my stable pools. Viruses containing BRAF gRNA (BRDN0000561167 and BRDN0001146266) ( 76479... different regions of your gene. I used existing BRAF gRNA viruses, but you can find a list of validated...

...et al., 2009).  Zebrafish behaviors Several behavioral assays can be done on zebrafish at 5 days post ...a high degree of control over time and space. Zebrafish have become a popular model organism because their...well to studies of neuroscience. The larvae of zebrafish are translucent and allow for noninvasive live...short generation times and high fecundity, make zebrafish ideal for high throughput studies of optogenetics...optogenetics.  Common opsins used in zebrafish Optogenetics functions through opsins which are light sensitive...to vertebrates and are commonly introduced in zebrafish through the UAS/Gal4 system for behavioral studies...by acoustic, visual or tactile stimuli, larval zebrafish react by performing a distinct C-bend to escape...

...development. Why have zebrafish become such a popular model organism? Zebrafish (Figure 1) were initially...biology that arose from zebrafish-based investigations. Genetic resources for zebrafish A common approach ...implemented in zebrafish. Developmental biology insights using lineage tracing in zebrafish To highlight...control this process. Additional zebrafish applications and conclusions Zebrafish are also popular outside of...Friedrich Miescher Lab of the Max Planck Society. Zebrafish (Danio rerio) have been used since the 1930’s ...maintain. Also in contrast to mice, the transparent zebrafish embryo develops outside of the mother, making ...embryogenesis. To illustrate some of the reasons that zebrafish have been embraced as a model organism, I’ll outline...

... requested gRNAs including those targeting EGFP, BRAF, and MAP3K4. The gRNAs targeting EGFP can be used...controls for gene editing or gene activation, while the BRAF and MAP3K4 gRNAs allow you to target these widely...

...make melanoma cells resistant to treatment with a BRAF inhibitor. (The test was the perfect complement ...

... al. "Mouse cutaneous melanoma induced by mutant BRaf arises from expansion and dedifferentiation of mature...stage. Zebrabow, or “zebrafish brainbow,” is a collection of transgenic zebrafish lines that allow for...(Hadjieconomou et al., 2011). Cell tracing in zebrafish with Zebrabow Before the generation of Zebrabow...(Pan et al., 2011), Brainbow was also used in zebrafish by injecting a CMV-Brainbow construct at the embryonic...Albert, et al. "Multicolor Brainbow imaging in zebrafish." Cold Spring Harbor Protocols 2011.1 (2011): ...

...repository for zebrafish strains and materials and information about zebrafish research. China Zebrafish Resource...experimentally validated in zebrafish. Zebrafish expression atlas - A zebrafish-specific gene expression ...Addgene's zebrafish resources: Find plasmids for use in zebrafish. Plasmid... Addgene for use in zebrafish. Browse Plasmids Plasmid Tools for Use in Zebrafish Looking for plasmids...your zebrafish gene of interest? Search Addgene's collection for plasmids that contain a zebrafish gene...Gene Disruption in Zebrafish - Leonard Zon Lab Programmable base editing of zebrafish genome using a modified...origin in zebrafish can be found below: Optimized QF-binary expression system for use in zebrafish - Bret...

... CRISPR Zebrafish CRISPR Plasmids: Zebrafish Browse CRISPR Plasmids ...been designed for use in zebrafish. CRISPR...Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps...CRISPR plasmids have been designed for use in zebrafish. Cut Fully functional CRISPR/Cas enzymes will ...

...AVEXIS for screening in zebrafish.... Plasmid Collections EXPRESs Plasmids Zebrafish Receptors EXPRESs Plasmids...Plasmids for Zebrafish Receptors You may also like... Gavin Wright Lab plasmids This is a collection of ...function in early vertebrate development using the zebrafish as a model organism. Please see the following ...

...TALEN plasmids targeting zebrafish genes.... TALengineering Reagents TALEN Zebrafish Reagents TALENs for Endogenous...Endogenous Zebrafish Gene Targets Recent reports have shown that engineered TALENs can be used to induce...induce targeted mutations with high efficiency in zebrafish ( Sander et al., Nat Biotechnol. 2011 ; Huang ...MGH to target sites within various endogenous zebrafish genes; many of these were generated as part of...effort to provide gene-editing nucleases to the zebrafish community. Target Gene Name TALENs Full Target...

... Collections EXPRESs Plasmids Zebrafish Monoclonal Antibodies Zebrafish Monoclonal Antibodies You may ...plasmids that encode monoclonal antibodies to zebrafish cell surface receptors and secreted proteins. ...heavy chains of mouse monoclonal antibodies to zebrafish proteins and the antibodies are produced by transfecting...8:76. PubMed 20525357 Plasmid ID Plasmid Name Zebrafish Gene 28214 SI1-Unc5b Unc5b 28215 SI2-Unc5b Unc5b...

... expression plasmids for Zebrafish... CRISPR Joung Lab CRISPR Plasmids Zebrafish CRISPR-Cas/RGN expression...expression plasmids for Zebrafish You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR...CRISPR-Cas/RNA-Guided Nuclease ( RGN ) technology in zebrafish. The Joung Lab has demonstrated the use of these...modify nine different target sites in endogenous zebrafish genes (Hwang & Fu et al., Nat Biotechnol. 2013...producing mRNA suitable for expression of Cas9 in zebrafish can be found in Hwang & Fu et al., Nat Biotechnol...

...DR299 (gRNA targeted to zebrafish gene apoea ) 42242 DR336 (gRNA targeted to zebrafish gene drd3 ) 42243 DR279...gRNA targeted to zebrafish gene fh [site #1]) 42244 DR302 (gRNA targeted to zebrafish gene fh [site #2...DR338 (gRNA targeted to zebrafish gene gsk3b ) 42246 DR311 (gRNA targeted to zebrafish gene rgs4 ) 42247 DR317...DR317 (gRNA targeted to zebrafish gene th1 ) 42248 DR330 (gRNA targeted to zebrafish gene tia1l ) 42249 DR333...Plasmids for Endogenous Zebrafish Genes CRISPR... CRISPR Joung Lab CRISPR Plasmids Zebrafish gRNA CRISPR-Cas/RGN expression... expression plasmids for Zebrafish You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools...

... family, small GTP binding protein Rac) RAF AFAF BRAF RAF1 Serine/threonine kinases: A-Raf proto-oncogene...

...compounds in one experiment. Zebrafish (Danio rerio) Since the 1960s, the zebrafish has gradually become an...with a disease have a homolog in zebrafish (Howe et al., 2013). Zebrafish are small, easily maintained as...easily bred, and produce 50-300 eggs at a time. Zebrafish embryos are also laid and fertilized externally...their genomes or to create transgenic animals. Zebrafish are an ideal model organisms for embryonic development...differentiation of β-cells in the the developing zebrafish pancreas. They first tagged these cells using ...PMCID: PMC2736433. Howe, Kerstin, et al. "The zebrafish reference genome sequence and its relationship...

...cells.   Zebrafish experiments with MG-2I-dL5** were performed with embryos and adult fish. Zebrafish are ...experimental settings: HEK293 cell culture and in live zebrafish. For cell culture experiments, only dL5**-expressing....  The video below shows what happens to the zebrafish’s heartbeat and blood circulation of following ...

..., generate a dedicated transgenic line. Using zebrafish as a model organism, the Parton Lab recently created..., termed BLITZ (Biotin Labeling In Transgenic Zebrafish) (Xiong, et al., 2021).    Fig. 1: Schematic...be co-expressed in essentially any transgenic zebrafish line expressing a GFP-tagged version of a protein... BLITZ system was designed and optimized with zebrafish in mind, it can easily be applied to other systems...

...reporter for editing enrichment (TREE) The Wang and Brafman lab developed a transient reporter for editing ...specific loci of interest in the genome. The Wang and Brafman lab showed that GFP positive cells isolated with...editing efficiency. TLDR: TREE from the Wang and Brafman lab uses an engineered BFP variant that converts...SJ, Brookhouser N, Schwarz G, Nguyen T, Wang X, Brafman DA (2019) A transient reporter for editing enrichment...

...and lineage tracing in other systems, such as zebrafish and Drosophila. Why Brainbow? Brainbow highlights... study model organisms such as Drosophila and zebrafish. Brainbow in Drosophila has aided in the mapping...motor neurons and the neuromuscular junction. In zebrafish, the method has become very useful in lineage ...labeling for cell tracing and lineage analysis in zebrafish. Pan YA, Freundlich T, Weissman TA, Schoppik D...

...currently shared amongst the zebrafish community. Don E, et al. Zebrafish. 2017. PubMed PMID: 27631880...allow scientists to quickly and easily generate zebrafish transgenic lines to study neurobiology and neuorodgenerative...in ribonucleoprotein particles (RNPs) to both zebrafish embryos and the mouse inner ear. These exciting...