...available for neuron-specific expression in both zebrafish and mammalian systems. The GCaMP6, GCaMP7, and...

...Kiani S, Guzman CD, Wiegand DJ, Ter-Ovanesyan D, Braff JL, Davidsohn N, Housden BE, Perrimon N, Weiss R...

... taurus (bovine), X. laevis (frog), D. rerio (zebrafish), D. melanogaster (fly), C. elegans (nematode)...

.... You can also use the library of full-length zebrafish rab proteins from Rob Parton's Lab (University...

...from porcine teschovirus-1 in human cell lines, zebrafish and mice. Kim et al (PLoS One. 2011;6(4):e18556...

...of cleaving genomic DNA in mammalian cells and zebrafish embryos, and they can be used to modify genomic...

...Kiani S, Guzman CD, Wiegand DJ, Ter-Ovanesyan D, Braff JL, Davidsohn N, Housden BE, Perrimon N, Weiss R...

...used to generate transgenic animals, including zebrafish, mice, rats, and rabbits (Sandoval-Villegas et...

...in ribonucleoprotein particles (RNPs) to both zebrafish embryos and the mouse inner ear. Fourth-generation...

... Control of CRISPR/Cas9 Function in Cells and Zebrafish using Light‐Activated Guide RNA. Angew Chem Int...

...behavioral responses in model organisms like mice, zebrafish, and Drosophila. These tools have been instrumental...

...Kiani S, Guzman CD, Wiegand DJ, Ter-Ovanesyan D, Braff JL, Davidsohn N, Housden BE, Perrimon N, Weiss R...

...61391 pCS2-Cre.zf1 Cre codon modified for zebrafish CMV Zebrafish Burgess 61570 Pvalb-2A-Cre targeting vector...Venus Elastase Zebrafish Stainier 84033 pHD157 mCherry-Cre fusion and Venus fabp10 Zebrafish Stainier 85040...for lineage tracing and mosaic analysis in zebrafish Zebrafish Lewis Additional Resources Cre-dependent ... ins-cre_cryaa-Venus Cre-Venus fusion insulin Zebrafish Stainier 24593 AAV-pgk-Cre codon optimized Cre...Lentiviral Jacks 60877 pMAZe Cre and dtomato hsp70 Zebrafish Lewis 60930 pPL5071_TEF1*-Cre_URA3 Cre expressed...pME-ERT2-Cre-ERT2 Tamoxifen-inducible Cre None Zebrafish Stankunas 82696 pCRE-iRFP670 Cre and iRFP670 PGK...fli1aCreERT2 Cre-ERT2; endothelial reporter fli1a Zebrafish Mercader Huber 108995 pDM1488 Cre; NeoR act15 ...

...arrays targeted to various zebrafish genes. The table below lists zebrafish genes and associated pairs...A list of zebrafish genes and associated pairs of zinc finger arrays that can be used to generate ZFNs... Engineered Zinc Finger Arrays for Endogenous Zebrafish Gene Targets Recent published reports have shown...effective tools for making targeted mutations in zebrafish. With support from the NIH and guidance from ...have NOT yet been tested for activity as ZFNs in zebrafish. Target Gene Name Zinc Finger Arrays Engineering...and OZ534 OPEN OPEN cCGCTTCAGCggtctGTGGACTCGa zebrafish similar to grp151 (or gpcr-2037) OZ535 and OZ536...finger arrays in a publication should notify zebrafishzfs@zincfingers.org and acknowledge NIH grant number...

...pST1374 (for expression in mammalian cells or zebrafish) pDW1775 (for expression in plants) Vectors encoding...al., Nat Biotech 2007 ) in mammalian cells or zebrafish (can be used as a substitute for pMLM335 described...al., Nat Biotech 2007 ) in mammalian cells or zebrafish (can be used as a substitute for pMLM336 described...al., Nat Biotech 2007 ) in mammalian cells or zebrafish). pMLM802 (for expression of a ZFN targeted to...al., Nat Biotech 2007 ) in mammalian cells or zebrafish). pMG10 (a '“2-in-1” ZFN cassette for expression...al., Nucleic Acids Res ) in mammalian cells or zebrafish). pCLR2068 (for expression of a ZFN targeted to...al., Nat Methods 2011 ) in mammalian cells or zebrafish as described by Moore et al. PLoS One 2012 and...

...pCS2+8CCerulean - Zebrafish/Xenopus/C.elegans/Sea urchin pCS2+8NCerulean - Zebrafish/Xenopus/C.elegans...Expression pCS2+8CeGFP - Zebrafish/Xenopus/C.elegans/Sea urchin pCS2+8NeGFP - Zebrafish/Xenopus/C.elegans/Sea...pCS2+8CmCitrine - Zebrafish/Xenopus/C.elegans/Sea urchin pCS2+8NmCitrine - Zebrafish/Xenopus/C.elegans... pCS2+8CmOrange - Zebrafish/Xenopus/C.elegans/Sea urchin pCS2+8NmOrange - Zebrafish/Xenopus/C.elegans/... pCS2+8CmCherry - Zebrafish/Xenopus/C.elegans/Sea urchin pCS2+8NmCherry - Zebrafish/Xenopus/C.elegans/...of GFP and/or mCherry, with neomycin cassette Zebrafish, Sea urchin, Xenopus, and C. elegans Hamdoun Lab...

... Currently supports: Drosophila, Arabidopsis, zebrafish, C. elegans, mouse, human, rat, cow, dog, pig,...cerevisiae ), worm ( C. elegans ), fruit fly, zebrafish, mouse, rat, and human. CCTop - CRISPR/Cas9 target...tomato, frog ( X. laevis and X. tropicalis ), and zebrafish. Off-Spotter: tool for CRISPR/Cas design Program...Organisms include human, mouse, rat, chicken, frog, zebrafish, fly, worm, Japanese rice fish, maize, Arabidopsis...Arabidopsis, and rice. Proof-of-concept performed in zebrafish. ZiFiT Targeter Originally developed to identify... chicken, frog (X. tropicalis and X. laevis), zebrafish, sea squirt, Drosophila, C. elegans, Arabidopsis... Currently supports: Drosophila, Arabidopsis, zebrafish, C. elegans, mouse, human, rat, rabbit, pig, possum...

... pT7-gRNA 46759 Zebrafish BsmBI none S. pyogenes Chen and Wente DR274 42250 Zebrafish BsaI none S. pyogenes...U6:gRNA 63156 Zebrafish BseRI none S. pyogenes Zon pDestTol2pA2-U6:gRNA 63157 Zebrafish BseRI none S. ...Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps... Lu pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2 74009 Zebrafish U6 yes, cut S. pyogenes EGFP Del Bene pUAS:Cas9T2ACre...Cas9T2ACre;U6:sgRNA1;U6:sgRNA2 74010 Zebrafish U6 yes, cut S. pyogenes Cre Del Bene pTE4396 74041 Mammalian...Using Transgenic Expression of Cas9 and sgRNAs Zebrafish Plasmids for assembly of gRNA cassettes using ...based pGGDestTol2LC vector. Uses five distinct zebrafish U6 promoters for sgRNA expression. Chen pCFD4-...

...for efficiently modifying endogenous genes in zebrafish (Hwang and Fu et al., Nat Biotechnol. 2013). The...CRISPR-Cas/RGN expression plasmids for use in zebrafish Guide RNA expression plasmids for the EGFP Reporter... Guide RNA expression plasmids for endogenous zebrafish genes...

...Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps...bacteria, Drosophila, plants, C. elegans, yeast, zebrafish, and Xenopus. Mammalian Plasmid Gene/Insert Promoter...Insert Promoter Selectable Marker PI Publication Zebrafish Plasmid Gene/Insert Promoter Selectable Marker...