... great way to do this is to use CRY2-CIB1 clustering. Fuse either CRY2 or CIB1 to your protein of interest...change and binds the plasma membrane under blue light CRY2-CIB1 heterodimerizes or forms clusters under blue...other separately, then use blue light to generate CRY2-CIB1 oligomers that trap the fusion protein away...inhibition of Rab protein vesicle trafficking via CRY2-CIB1 clustering. Image adapted from Aljiboury et...vitro, opening up a wide realm of possibilities. The CRY2-SuperPLD module is optogenetically recruited by ...dynamics. Need a refresher on VVDfast, iLID, LOV, CRY2-CIB, and other optogenetic modules? Or want to find...

...  cryptochrome 2 (CRY2) from Arabidopsis thaliana. When exposed to blue light, CRY2 undergoes a conformational...the original version containing CRY2 without the extra peptide. CRY2clust should prove a useful tool to ...basic-helix-loop-helix) protein. After fusing the CRY2 and CIB1 domains to separate proteins of interest...control homo-oligomerization of a protein fused to CRY2, although some studies have shown that this clustering...optogenetics toolbox with CRY2clust Article contributed by Brook Pyhtila  Listen to the CRY2clust podcast segment...proteins in response to blue light. This tool, CRY2clust, was created by adding a 9-residue peptide to ...With its additional 9 amino acid peptide, the CRY2clust domain enables fast and reversible oligomerization...

...CIB1; and 2) the activator - the CRY2 photolyase homology region (CRY2PHR) fused to a transcriptional activator...and C-termini of dCas9; and 2) CRY2FL-VP64, a fusion of full-length CRY2 and the transcriptional activator...light-inducible heterodimerizing cryptochrome 2 (CRY2) and calcium and integrin-binding protein 1 (CIB1... figure below. Once triggered by blue light, the CRY2 and CIB1 proteins heterodimerize and move the activator...from naturally occurring photoactive proteins (i.e. CRY2 and Vivid) - Alexander Deiters’ lab, on the other...-induced Dimerization Nihongaki et al., 2015   CRY2 and CIB1 from A. thaliana dCas9 Activation of gene...gene transcription Polstein et al., 2015 LACE CRY2 and CIB1 from A. thaliana dCas9 Activation of gene...

...the addition of two light-sensitive ingredients - CRY2 and CIB1 – which they borrowed from Arabidopsis ...

...unique from previous iterations is the addition of CRY2, another Arabidoposis protein that heterodimerizes...

...minutes. Constructs using the photodimerizer system CRY2-CIB1 to regulate botulinum neurotoxin were also ...

...for yeast Gal1 Yeast Sandmeyer 26888 pmCherry-CRY2-CreN CRY2-CreN and mCherry coexpression; Light inducible...photo-activatable cre recombinase CAG AAV Bruchas 75268 pAAV-CRY2-CreN photo-activatable cre recombinase CAG AAV Bruchas...

... widely used Cry2-CIB1 system, light induces a conformational change in cryptochrome Cry2 to permit CIB1...protein of interest. A genetically-encoded nuclear Cry2 fusion can thus direct a CIB1-protein fusion to ...protein, such as the Cre recombinase, is fused to Cry2 and the other half to CIB1, light-stimulated heterodimerization...design of synthetic two-part transcription factors - Cry2 and CIB1 are fused to a transcriptional activation...effectors OPTOSTIM PHR domain2 of cryptochrome 2 (Cry2) from Arabidopsis thaliana is fused to truncated...

...protein domains CibN and Cry2 to inactive dCas9 and VP64, respectively. CibN and Cry2 form a heterodimer in...complex recruits a CIB1 binding protein (a portion of CRY2) fused to the p65 activation domain, which activates...cited below. Scientists can obtain dCas9-trCIB1, CRY2PHR-p65, and other plasmids from this article through...

...domains, phytochrome B (PhyB), and cryptochrome 2 (CRY2) (Zhang & Cui, 2015). When stimulated with light...

...IDR-mCherry-Cry2-NLS TBP SFFV Parkinson's Denes Hnisz 145270 pHR-TBP(53Q)-IDR-mCherry-Cry2-NLS TBP Parkinson's...101223 pHR-FUSN-mCh-Cry2WT FUS SFFV ALS Clifford Brangwynne 101224 pHR-FUSN-mCh-Cry2olig FUS SFFV ALS Clifford...Clifford Brangwynne 122441 pHR-FUSN-miRFP670-Cry2WT FUS Cry2WT, miRFP670 SFFV ALS Clifford Brangwynne 122668...Clifford Brangwynne 101226 pHR-HNRNPA1C-mCh-Cry2WT HNRNPA1 SFFV ALS Clifford Brangwynne 101792 pMXs-mPKR-FLAG-IG...VPS35 shRNA2 VPS35 Parkinson's Lei Lu 164203 CMV-CRY2oligo-mcherry-ANXA11 ANXA11 mCherry CMV ALS Jennifer...Jennifer Lippincott-Schwartz 164204 CMV-CRY2oligo-mcherry-ANXA11(D40G) ANXA11 mCherry CMV ALS Jennifer Lippincott-Schwartz...Lippincott-Schwartz 164205 CMV-CRY2oligo-mcherry-ANXA11(R235Q) ANXA11 mCherry CMV ALS Jennifer Lippincott-Schwartz...