...Bioluminescence Resonance Energy Transfer (BRET). BRET is similar to Förster Resonance Energy Transfer (FRET), ...fluorophore. The difference for BRET is that the energy transfer is between a luminescent donor and a fluorescent...protein dissociation by bioluminescence resonance energy transfer 2, or BRET2. With binding of the drug,...

...CellTagD13), which is when the iEP phenotype typically emerges. Every three days, a sample of cells was collected...expression changes of more than 100,000 cells undergoing iEP conversion, the Morris Lab found that cells... to uncover two distinct trajectories of MEFs undergoing iEP conversion, but CellTag could be used to ...study other biological processes where cells are undergoing identity changes, such as the differentiation...

...transposons, the Sternberg and Zhang labs developed CRISPR-transposons. The Sternberg lab's system called...and Gram-positive bacteria Jason Peters, Oren Rosenberg, and Carol Gross used two different genetic systems...References Bikard D, Euler CW, Jiang W, Nussenzweig PM, Goldberg GW, Duportet X, Fischetti VA, Marraffini LA (2014..., Engel JN, Dutton RJ, Grossman AD, Gross CA, Rosenberg OS (2019) Enabling genetic analysis of diverse...

...suddenly cool: DNA methylation. In 1983, Andrew Feinberg and Bert Vogelstein showed, for the first time...methylation detected in a human disease: cancer (Feinberg & Vogelstein 1983). The floodgates, as they say...methylated strand is diluted to undetectable amounts: ergo, DNA demethylation! With optimization, this method...827. https://doi.org/10.1038/s41588-020-0639-9.  Feinberg A, Vogelstein B (1983) Hypomethylation distinguishes...

...Health Organization has declared a global health emergency and called for the fast-tracked development of... was underscored by the potential scale of the emerging outbreak, as many as 2.2 billion people globally...prototyping and sensor assembly pipeline for future emerging pathogens or health crises. Many thanks to our..., and self-assembly for applications in health, energy, and sustainability.   References 1. Pardee, Keith...

... visible and infrared light to dissipate excess energy at wavelengths of low photosynthetic activity. ...lifetime imaging microscopy-based Förster resonance energy transfer (FLIM-FRET) in living cells (Figure 4)...10.1074/jbc.m606921200 Dove SG, Takabayashi M, Hoegh-Guldberg O (1995) Isolation and Partial Characterization...48604-4 Salih A, Larkum A, Cox G, Kühl M, Hoegh-Guldberg O (2000) Fluorescent pigments in corals are photoprotective...

...fluorescent proteins (Gambarotto, et al. 2019; Tillberg, et al. 2016). That being said, the anchoring,.../doi.org/10.1371/journal.pbio.3001020  Chen F, Tillberg PW, Boyden ES (2015) Expansion microscopy. Science...//doi.org/10.7554/elife.73775  Dani A, Huang B, Bergan J, et al (2010) Superresolution Imaging of Chemical...101796. https://doi.org/10.1016/j.mex.2022.101796  Tillberg PW, Chen F, Piatkevich KD, et al (2016) Protein-retention...

...real goal. We are all constantly negotiating our energy and attention. The goal is to maximise the time...Check out Joanne's Reddit AMA Managing time and energy between work and the rest of life is sometimes ...will worry less about how they will handle an emergency and this will make you much more confident about...daughter vehemently reminded her doctor that she was allergic to penicillin – I am much less worried knowing...

...and expand our current knowledge. The Abudayyeh-Gootenberg lab (also called the AbuGoot lab), a joint lab...lab run by Omar Abudayyeh, PhD, and Jonathan Gootenberg, PhD, focuses exclusively on this space — developing...Gardner A, Makarova KS, Koonin EV, Abudayyeh OO, Gootenberg JS. Programmable RNA targeting with the single-protein...Adrianna E. Zepeda, Kevin Holden, Jonathan S. Gootenberg, Omar O. Abudayyeh. Drag-and-drop genome insertion...

...update of this post. References Abudayyeh OO, Gootenberg JS, Essletzbichler P, Han S, Joung J, Belanto...://doi.org/10.1186/s13059-018-1591-1 Cox DBT, Gootenberg JS, Abudayyeh OO, Franklin B, Kellner MJ, Joung...nature26155 Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova...://doi.org/10.1126/science.aba8853 Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, ...

...specific neuronal subtypes such as inhibitory GABA-ergic interneurons, Gordon Fishell’s lab has developed... you to target and functionally manipulate GABA-ergic interneurons across species and will undoubtedly...You can find the AAV vectors for infecting GABA-ergic interneurons here. Dimidschstein J, et al. Nature... use as a donor in FRET (fluorescence resonance energy transfer) and FLIM (Fluorescence Lifetime Imaging...Under the supervision of Dr. Tan, a team of undergraduates created DNA ladders (aka DNA molecular weight...

...through a process known as bioluminescent resonance energy transfer (BRET). The fluor then emits light at ...release have been made using fluorescent resonance energy transfer (FRET) probes. These probes generally ...FRET signal. FRET is a non-radiative transfer of energy from one fluorescent protein to a compatible fluorescent...other fluorescent probes but can still transfer energy to the fluorescent protein acceptor. NLuc is an...

...: 797459. Berg CM, Liu L, Coon M, Strausbaugh LD, Gray P, Vartak NB, Brown M, Talbot D, Berg DE. pBR322...frequently in plasmids with large inserts (>7 kb)(Berg et al., 1989), direct repeats (Ribeiro et al., 2009...and define recA-independent mechanisms. Avoid overgrowing the bacterial culture Another way to prevent ...

...here . pAra Series Various Plasmids pBAD GFP Bryan Berger Reporter system for testing hetero- and homo-dimer...

...light-sensitive proteins that absorb photons for energy conversion or the induction of intra- or intercellular...shorter than 5 kb (Hirsch et al., 2016; Hudry and Vandenberghe, 2019). How to achieve strong opsin expression...PubMed PMID: 26135556. Hudry, Eloise, and Luk H. Vandenberghe. "Therapeutic AAV gene transfer to the nervous...Juarez, Barbara, and Ming-Hu Han. "Diversity of dopaminergic neural circuits in response to drug exposure...

...emitting a photon with slightly less energy due to non-radiative energy losses. This difference in emission...ensure accurate tagging. In Fluorescence Resonance Energy Transfer (FRET) applications, the dyes need to ...excitation and emission profiles to enable effective energy transfer, which is key to observing interactions...

...complementation in plants. Bioluminescence Resonance Energy Transfer (BRET) CalFlux VNT : An intracellular ...

...discovered and studied but it is only the tip of the iceberg as it has been predicted that many others are still...microbial genome and metagenome from the Department of Energy’s Joint Genome Institute for uncharacterized genes...gene together with the adjacent CRISPR array and intergenic regions containing the putative tracrRNA into...

... its confines. The beauty of serendipity often emerges at the intersections of disciplines. Cast your ... capture fleeting thoughts and connections that emerge during moments of inspiration. Additionally, journaling...serendipity. However, the best discoveries often emerge when we allow ourselves the freedom to explore ...

...doing and presenting undergraduate research at BU, I realized that I’m energized by talking to people ... to ask store clerks for help at the mall. In undergrad I was forced to open up a little after I dropped...