...negative selection strategy uses the toxin CcdB. Gateway cloning takes advantage of this property by including...

...RESOLUTE Plasmid Collection You may also like... Gateway Cloning Brain Initiative Collection NeuroMab Collection...codon-optimized ORF sequences for human SLCs in Gateway-compatible pENTR vectors (pDONR221). These vectors...and efficient sub-cloning into a wide range of Gateway-compatible pDEST vectors. ID Plasmid Gene/Insert...

... plasmid. Cheng 17452 pLenti CMV Puro DEST 3rd Gateway destination plasmid with constitutive or inducible... Tet-based inducible shRNA or cDNA expression, gateway plasmid, constitutive Hygromycin resistance gene...of pSLIK. Fraser 15948 pLOVE 3rd Tet inducible gateway destination plasmid for cDNA expression Ramalho-Santos...PGK driven puromycin Sabatini 25895 pLX301 3rd Gateway plasmid for CMV driven expression of cDNA. See ...pCW57.1 3rd Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-puro. See article for more versions...

...Additional resources on the Addgene blog Learn about Gateway cloning Read about genome engineering in flies ...

...methods (restriction enzyme, ligation independent, Gateway, Gibson, etc). The cloning method is ultimately...

...inactivates or completely removes (in the case of Gateway cloning) one cassette. Counterselection allows ...

...chemical stimulation was sucrose…..sounds like the gateway to chocolate and peanut butter to me. What’s on...

... - Borodina Lab Golden GATEway cloning system - Wittbrodt Lab MultiSite Gateway Kit : Creating three-part...oleaginous yeast Y. lipolytica - Rossignol Lab Yeast Gateway kit : 285 vectors for expression, markers, fusion...

...repression), and Fok1-dCas9 (dimeric nuclease). Gateway assembly method Frew Lab Multiple Lentiviral Expression...should be compatible with type IIS enzyme BfuAI. Gateway cloning is then used to combine the multiple gRNAs...

...amounts of cloning. Assembly is scarless, unlike Gateway cloning, and the method’s flexibility allows it...

...example is the ccdB gene system commonly used in Gateway® Cloning. There are also methods to select for ...

...Resources on the Addgene Blog Learn More about Gateway Cloning Gibson Assembly and Other Long-Homology...

...part of our Genetic Pathway Reference Set. These Gateway-compatible entry clones are the product of many...

...74334 pCR8-Magneto2.0-p2A-mCherry Gateway 74333 pCR8-Magneto2.0 Gateway 74307 pAAV-CMV-DIO-Magneto2.0...The collection is compatible with both standard Gateway cloning and the FNLCR Combinatorial Cloning Platform...mammalian systems. The DULIP system is comprised of Gateway-compatible plasmids in which “bait” proteins are...

...Lentiviral vector for inducible expression for Gateway cloning; selection cassette in format: PGK-rtTA...Doyon 58245 pGLTR-X-GFP Single vector lentiviral Gateway RNAi system for conditional cell line generation... Off Verma 26803 pEnt L1L3 EF1a-tTA-2 Contains Gateway L1L3 sites and EF1α promoter driving tTA; contains...

...of protein in bacterial cells: the pET, pRSET, Gateway pDEST, and pBAD vectors for example. Protein expression...

...may require strains such as ccdB survival (for Gateway plasmids).  We also like to store plasmids that...

...collection is a set of vectors for use with the Gateway Cloning Platform (Life Technologies, Carlsbad, ...

...required. pET-HIS-Sangamo 40786 TALEN Gang Bao Gateway cloning and expression of an N-terminally His-tagged...endonuclease domain. pET-Sangamo-His 40787 TALEN Gang Bao Gateway cloning and expression of a C-terminally His-tagged...N-terminal 6x His tag and an MBP tag. Compatible with Gateway cloning. pDEST-periHisMBP 11086 Tac (lactose/IPTG...formation or prevent toxicity. Compatible with Gateway cloning. pGTvL1-SGC 39188 pTac (lactose/IPTG inducible...

...transcription. The HIS3 reporter system (e.g. the Gateway-compatible bait Y2H plasmid pEZY202  from Yu-Zhu...