Truncated gRNAs for Regulating Gene Expression
Type
Blog Post
...appropriately designed small RNA, or guide RNA (gRNA). The gRNA conventionally contains a 20-nucleotide sequence...along with the necessary gRNAs, but it would not be possible to control which gRNA becomes complexed with...truncated gRNAs
An alternative approach is to modulate Cas9’s nuclease activity by modifying the gRNA with ...employed 20nt gRNAs. It should be noted, however, that on rare occasions truncated gRNAs (≤15nt) have ...recognize and bind to DNA; when complexed with a gRNA that directs them to the promoter of a gene of interest...species, each of which interacts with a distinct gRNA that allows users to decide which Cas9 protein is...protein itself. When native Cas9 is complexed with a gRNA that has been truncated such that it exhibits 15...