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We narrowed to 9 results for: human h2b gfp

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Showing: 1 - 9 of 9 results

  1. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...mutate in the genome) and an out-of-frame H2B (histone)-GFP reporter into the genome of mammalian cells...puromycin resistance gene linked to an in-frame H2B-GFP. This visual read out allows for easy screening... the supplied donor plasmid will express GFP. If there’s GFP expression, presumably the endogenous target...targeted the gene NFE2L2, the Madison lab found that H2B-GFP expressing cells exhibited a 2-4 fold increase ...RFP to GFP expressing cells provides a real-time read out of Cas9 activity.  By using the RFP:GFP ratio... can easily and seamlessly remove the integrated GFP reporter leaving you with just your desired genomic...target a specific locus to create a knock-out in human embryonic stem cells.  Find GEmCherry2 reporter ...

  2. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ... Gerlich 25999 LV-GFP Chromatin H2B GFP Elaine Fuchs 11680 H2B-GFP Chromatin H2B GFP Geoff Wahl 21217 ...pKanCMV-mRuby3-10aa-H2B Chromatin H2B mRuby3 Michael Lin 224436 pCS2FA-H2B-mScarlet3 Chromatin H2B mScarlet3 Dorus...21217 PGK-H2BmCherry Chromatin H2B mCherry Mark Mercola 20972 H2B-mCherry Chromatin H2B mCherry Robert Benezra...61803 GFP-Rab7A Endosomes (late) Rab7a AcGFP Gia Voeltz 12605 GFP-rab7 WT Endosomes (late) RAB7 GFP Richard...Chromatin H2B mGreenLantern Gregory Petsko 74257 pKanCMV-mClover3-10aa-H2B Chromatin H2B mClover3 Michael Lin...pHIV-H2BmRFP Chromatin H2B mRFP Bryan Welm , Zena Werb 21045 pH2B_mCherry_IRES_puro2 Chromatin H2B mCherry...136571 pH2B-emiRFP670 Chromatin H2B emiRFP670 Vladislav Verkhusha 136567 pH2B-emiRFP703 Chromatin H2B emiRFP703...

  3. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...comprehensive comparison to GFP, please refer to NEB's comparison of SNAP-tag, CLIP-tag, and GFP . Technology SNAP-tag...SNAP-tag SNAP-tag is based on the human O6-alkylguanine-DNA-alkyltransferase (hAGT), a DNA repair protein...Control for mitochondrial localization 101124 pSNAPf-H2B Control Plasmid SNAP-tag Control for nuclear localization...and transient mammalian expression 101134 pCLIPf-H2B Control Plasmid CLIP-tag Control for nuclear localization...

  4. Caltech Systemic Capsids

    Type
    Collection
    ...Dimidschstein 135631 pAAV-S5E2-GFP-fGFP E2 regulatory element GFP, membrane-targeted GFP Control Dimidschstein ...pAAV-CAG-GFP CAG GFP Control Boyden 50465 pAAV-hSyn-EGFP hSyn EGFP Control Roth 50469 pAAV-CaMKIIa-EGFP CamKIIa...104061 CAG-NLS-GFP CAG NLS-GFP Control Gradinaru 105530 pAAV.CMV.PI.EGFP.WPRE.bGH CMV EGFP Control Wilson...Ting 163509 CN1839-rAAV-hSyn1-SYFP2-10aa-H2B-WPRE3-BGHpA hSyn1 H2B-SYFP2 Control Ting 191706 AiP13044 - pAAV-AiE0779m...Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control Boyden MaCPNS2 These viral vector preparations...Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control Boyden CAP-B10 These viral vector preparations...Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control Boyden CAP-B22 These viral vector preparations...

  5. Brain Armamentarium

    Type
    Collection
    ...James M. Wilson 37825-CAP-B10 pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward Boyden Viviana...Viviana Gradinaru 37825-CAP-B22 pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward Boyden Viviana... Gradinaru 37825-CAP-MaCPNS1 pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward Boyden Viviana... Gradinaru 37825-CAP-MaCPNS2 pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward Boyden Viviana... in studying circuits in laboratory animals and human tissue specimens. These technologies have the potential...: pAAV-AiE0873m_3xC2-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN4496) Expression of SYFP2 (...Gradinaru 163509-PHPeB CN1839-rAAV-hSyn1-SYFP2-10aa-H2B-WPRE3-BGHpA AAV vector for nuclear SYFP2 labeling...

  6. Brain Initiative Collection

    Type
    Collection
    ...Fishell 83895-AAV1 pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP expression in forebrain GABA-...Fishell 83895-AAV2 pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP expression in forebrain GABA-...Fishell 83895-AAV8 pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP expression in forebrain GABA-...Fishell 83895-AAV9 pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP expression in forebrain GABA-...Fishell 83895-AAVrg pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP expression in forebrain GABA-...element Gordon Fishell 83900-AAV1 pAAV-mDlx-GFP-Fishell-1 GFP expression in forebrain GABA-ergic interneurons...element Gordon Fishell 83900-AAV2 pAAV-mDlx-GFP-Fishell-1 GFP expression in forebrain GABA-ergic interneurons...

  7. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...both the GFP and the RFP proteins in the nucleus. During the first round of translation, the GFP proteins...Compartment mCherry-Rab5 BFP-Rab5 GFP-Rab5B Vacuolar & Budding Compartment GFP-Rab4B Mitochondria mito-BFP...GTPase in division) GFP-Mff (outer membrane protein) Late Endosome mCherry-Rab7A GFP-Rab7A     ...2015). He has also constructed a library of eGFP-tagged human ORF clones to allow testing and comparison...protein Knock-off, is made up of three components: a GFP protein that binds to the coding region of a reporter...reporter mRNA via a PP7 coat protein (NLS-PCP-GFP) a RFP protein that binds to the 3’ UTR of the reporter...thermophilus which have been used for gene editing in human cells without cross-talk in cognate sgRNA binding...

  8. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ... Tagging in Human Cells on the Addgene Blog. Plasmids for Endogenous Gene Tagging in Human Cells Find ...passaging of human iPS cells (Link opens in a new window) RNP transfection for gene editing human iPS cells...Histone H2B type 1-J Histones 109119 CTNNB1-mEGFP AICSDP-47 mEGFP Beta-catenin Adherens junctions 107579 RAB5A-mEGFP...fluorescently tagged proteins of key cellular structures in human cell lines. Depositor... opens in a new window) of fluorescently-tagged human induced pluripotent stem cell (hiPSC) lines. The...fluorescently tagged proteins of key cellular structures in human cell lines of their choice. For a full description...). Systematic gene tagging using CRISPR/Cas9 in human stem cells to illuminate cell organization. Mol ...

  9. Tetracycline Inducible Expression

    Type
    Collection
    ...Iwasato 63704 pRetroX GFP T2A Cre Retroviral vector for dox-inducible expression of GFP T2A Cre recombinase...Eric Kowarz 96930 XLone-GFP Tet-On PiggyBac vector for inducble expression of EGFP Tet-On 3G rtTA TRE3GS...171123 pLVX-TetOne-Puro-GFP Lentiviral Tet-On vector for inducible expression of EGFP Tet-On 3G rtTA TRE3GS...51543 FUW-tetO-hOKMS Tet-On inducible expression of human Oct4, Sox2, Klf4, and Myc for iPS cell generation...differentiating iPSCs into glutamatergic neurons via human NGN2 expression. See more from the iPSC Neurodegenerative...pUCM-AAVS1-TO-hNGN2 Introduction of dox-inducible human NGN2 into AAVS1 safe harbor site, for iPSC differentiation... is monitored by the dilution of a dox-inducible H2B-mCherry marker. Aviv Regev , Joan Brugge Return to...
Showing: 1 - 9 of 9 results