...immunology collection contains plasmids for genes involved in innate and adaptive immune signaling and cell-cell...such as macrophages and neutrophils that attack invading pathogens. Innate immune cells also process and...membrane and can also function as signaling proteins involved in proliferation, cell differentiation, and apoptosis...molecule, major histocompatibility complex, class II invariant chain DHLAG, HLADG, Ia-GAMMA CD8A CD8a molecule...

...those pages. Several companies, such as Qiagen , Invitrogen , and Promega sell kits for isolating plasmid...fully resuspended. Add 200 μL of Solution II and invert the tube carefully 5 times to mix the contents.... μL of cold Solution III to each tube. Mix by inverting several times. A white precipitate will be formed...reactions. Air dry the pellet (can be done by inverting the tube at an angle over kimwipe) for 20-30 min...ethanol and 1/10 volume of 3 M Na-acetate (pH 4.8). Invert the microfuge tube to mix. (Optional) Place the... Pour out the supernatant in the sink. Open and invert the tubes on a paper towel to drain them out. Wash...about losing the pellet. Dry with vacuum or by inverting over paper towel for 5-20 min. Resuspend dry DNA...

...valued opportunities for further translational investment. All resources and high-quality research tools...

...for cell migration research at Addgene. CMC investigators who have deposited plasmids include: Joan Brugge...

...Plasmids Fluorescent Protein Purpose Principal Investigator Plasmids GFP GFP-dependent transcription factors...

... 1mm thick, 10-well, Invitrogen NP0321BOX 20X MOPS SDS running buffer, Invitrogen NP0001 4X NuPage sample...buffer, Invitrogen NP0007 10X NuPage sample reducing agent (500 mM dithiothreitol, DTT), Invitrogen NP0009...NP0009 PageRuler Plus Prestained protein ladder, Invitrogen 26619 Pipette tips, 10 µL, VWR 76322-132 Pipette...MOPS buffer into 475 mL of deionized water. Gently invert to mix. Procedure Section 1: SDS-PAGE Add 5 µL ...

...parasite-parasite interactions that function in the invasion of the human erythrocyte by the blood stage of...

...signaling pathway, focusing on the main pathways involved in cancer development. These pathways are important...

...Currently supports over 20 model and non-model invertebrate species. These plasmids are described in: Genome...

...Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . Don’t See What You’re Looking For? Please...

...colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created...

...optimized protocols we use to produce our AAV inventory . See an overview of our viral vector production...

...Basigin is a receptor essential for erythrocyte invasion by Plasmodium falciparum. Crosnier C, Bustamante...

...produced by several methods, the most common of which involves homologous recombination of adenovirus plasmids...

...Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . No available items found to match all requested...

...serum-free media Invitrogen: #31985 Dulbecco’s Modified Eagle Medium (DMEM) Invitrogen: #11995 Fetal Bovine...Bovine Serum (FBS) Invitrogen: #16000 Penicillin/Streptomycin Invitrogen: #15140-122 Polypropylene tubes... buffer NEB #B0202S DH5 alpha competent cells Invitrogen #18258-012 Qiaquick gel extraction kit Qiagen...homologous recombination of unstable regions, such as Invitrogen Stbl3™ or NEB Stable cells. This will ensure ...

...referred to as a minimal triad, with each component involved in GTPase signaling activity. Locations of ARF...

...receptor-ligand interactions that function in the invasion of the human erythrocyte by the blood stage of...

...to mutate a specific gene or your anxiously investigating gene therapy options for a rare disease in humans...

...to genetics, high-throughput screening and non-invasive live imaging. A standard toolbox of genetic methods...