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We narrowed to 20 results for: keith joung

Showing: 1 - 20 of 20 results

  1. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...pyogenes Cas9s with novel PAM recognition In 2015, Keith Joung’s lab performed a series of positive selection...Abudayyeh OO, Gootenberg JS, Essletzbichler P, Han S, Joung J, Belanto JJ, Verdine V, Cox DBT, Kellner MJ, Regev...Gootenberg JS, Abudayyeh OO, Franklin B, Kellner MJ, Joung J, Zhang F (2017) RNA editing with CRISPR-Cas13....Gonzales APW, Li Z, Peterson RT, Yeh J-RJ, Aryee MJ, Joung JK (2015) Engineered CRISPR-Cas9 nucleases with ...Nguyen NT, Welch MM, Lopez JM, McCaw ZR, Aryee MJ, Joung JK (2016) Genome-wide specificities of CRISPR-Cas...Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin EV, Zhang F (...

  2. What's New in CRISPR - May 2019

    Type
    Blog Post
    ... unwanted RNA editing (SECURE) base editors Keith Joung’s lab recently found that a cytosine base editor...

  6. Zinc Finger Consortium: OPEN Reagents

    Type
    Collection
    ...Reagents (Kit # 1000000013 ) Depositing Labs: Keith Joung This kit consists of plasmids and strains used...academics and nonprofits only. You may also like... Keith Joung Lab plasmids Daniel Voytas Lab plasmids Scot ...kit used in this publication was a gift from Keith Joung (Addgene kit # 1000000013)" For your Reference... ML, Thibodeau-Beganny S, Sander JD, Voytas DF, Joung JK. Nature Protocols . 2009;4(10):1471-501. doi:...requires OPEN pools (available by request from the Joung lab) and the following reagents (listed below and...1997 ) and later modified by Pabo and colleagues ( Joung et al., PNAS 2000 ). The Oligomerized Pool ENgineering...protocol for practicing OPEN is available in the 2009 Joung Nature Protocols paper . How to Cite this Kit These...

  7. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...PMCID: PMC3651036. 15. Tsai, Shengdar Q., and J. Keith Joung. "Defining and improving the genome-wide specificities...genotyping options. Figure reproduced from Tsai and Joung 2016 with permission. Even though progress has...experiments move closer to the clinic (Tsai and Joung 2016). Table 2: Unbiased Genotyping Options Technique...specificities of CRISPR–Cas9 nucleases”
by Tsai and Joung 2016. Many thanks to our guest blogger Søren Hough...

  8. Zinc Finger Consortium Reagents

    Type
    Collection
    ...Finger Consortium Reagents You may also like... Keith Joung Lab plasmids Daniel Voytas Lab plasmids Scot ...engineered zinc finger technology. Consortium members Keith Joung and Daniel Voytas have deposited at Addgene various...deposited by Zinc Finger Consortium members like the Joung Lab, Voytas Lab, and Wolfe Lab Plasmid...

  9. TALEN Engineering

    Type
    Collection
    ...TALengineering Reagents Joung Lab TAL Effector Engineering Reagents You may also like... Keith Joung Lab plasmids...Reagents from the Keith Joung laboratory for engineering TAL effectors, including designed TALENs, and... Daniel Voytas Lab plasmids CRISPR plasmids The Joung Lab has developed three platforms for engineering...pages describing these reagents are provided below: Joung Lab REAL Assembly TALEN Kit Individual REAL TALE...

  11. Individual REAL TALE Repeat Plasmids

    Type
    Collection
    ...You may also like... Keith Joung Lab plasmids Daniel Voytas Lab plasmids The Joung Lab recently described...available individually or as a set as part of the Joung Lab REAL Assembly TALEN Kit , which also includes...

  12. TALEN Expression Vectors

    Type
    Collection
    ...REAL, REAL-Fast and FLASH You may also like... Keith Joung Lab plasmids Daniel Voytas Lab plasmids CRISPR...purchased together with the Joung Lab Individual REAL TALE Repeat Plasmids in the Joung Lab REAL Assembly TALEN...

  14. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...transversion editors starts with researchers in the Keith Joung lab, who were engineering an ABE to reduce its...Miller, B. R., Langner, L. M., Grünewald, J., & Joung, J. K. (2021). CRISPR C-to-G base editors for inducing...

  15. TALEN Guide

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    Collection
    ... best-selling kit in Addgene’s history. Dr. Keith Joung’s lab at Massachusetts General Hospital also recently...serial ligation protocol to assemble arrays. Dr. Joung, co-founder of the Zinc Finger Consortium with Dr...Sander JD, Cade L, Khayter C, Reyon D, Peterson RT, Joung JK, Yeh JR. Nat Biotechnol. 2011 Aug 5;29(8):697...

  16. TALEN Plasmids and Kits

    Type
    Collection
    ...ligation. Validated in zebrafish somatic cells. Keith Joung Zhang Lab TALE Toolbox PCR/Golden Gate cloning...in multiple organisms. Dan Voytas Adam Bogdanove Joung Lab TAL Effector Engineering Reagents Assembly via... and mouse pluripotent stem cells. 48706 pTAL7b Joung Lab REAL Assembly TALEN Kit 49042 EMM65 Bradley ...

  17. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...adenine and cytosine editing components together. Keith Joung’s lab created a dual-deaminase editor called SPACE...R., Langner, L. M., Hsu, J. Y., Aryee, M. J., & Joung, J. K. (2020). A dual-deaminase CRISPR base editor...

  19. Zebrafish Plasmid Collection

    Type
    Collection
    ...-Guided Nuclease (RGN) expression plasmids - Keith Joung Lab CRISPR/Cas9-based conditional mutagenesis...

  20. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...UV5 Alzheimer's Keith Joung 35218 psen2_R (OZ578) PSEN2 lac UV5 Alzheimer's Keith Joung 35946 LRRK2-K1906M...Parkinson's Keith Joung 36005 Park2_R (TAL 3013) PRKN Flag, FOKI CMV Parkinson's Keith Joung 36046 pT7-...telangiectasia Keith Joung 36806 TAL2403 ATM Flag, FOKI CMV Ataxia telangiectasia Keith Joung 36939 pcDNA3...CMV Parkinson's Keith Joung 42798 TAL3335 PRKN Flag, FOKI CMV Parkinson's Keith Joung 42801 TAL3338 PINK1...CMV Parkinson's Keith Joung 42802 TAL3339 PINK1 Flag, FOKI CMV Parkinson's Keith Joung 42934 pPET-PKR/...
Showing: 1 - 20 of 20 results