...246-54. Dowdy Adenovirus Mouse Non-integrating expression of mouse Sox2, Oct4, c-Myc, and Klf4 from four...Hochedlinger Lentivirus Mouse Doxycycline-inducible expression of mouse Oct4, Sox2, Klf4, and c-Myc from four separate...Lentivirus Mouse Doxycycline-inducible lentiviral expression of mouse Oct4, Sox2, Klf4, and c-Myc from ...for generating mouse iPS cells; retroviral expression of mouse Sox2, Oct3/4, Klf4, and c-Myc from separate...Woltjen Plasmid Mouse Non-integrating polycistronic expression of mouse Oct4, Klf4, Sox2, and c-Myc from two...episome Mouse Non-integrating EBNA1-mediated polycistronic expression of mouse Oct4, Sox2, Klf4, c-Myc, ...reprogramming of mouse somatic cells. Cell Stem Cell. 2008 Feb 7. 2(2):151-9. Jaenisch Lentivirus Mouse Polycistronic...

...M7443) mouse antibody from Sigma (Link opens in a new window) C-terminal Anti-MECP2 (M6818) mouse antibody...diagnostic criteria, defined as the presence of relatively normal early development, regression with a loss...top Animal Models Studies of the MECP2 protein in mouse and rat models have accelerated our understanding...Search (Link opens in a new window) JAX Mice for mouse models that include MECP2 null alleles or common...following the link to their laboratory website. Mouse Line Mutation (DNA) Background Strain Purpose Publication...endogenous MECP2 locus Castaneus MECP2-NLuc-tdTomato mouse reporter cell line (Link opens in a new window) ...Conditional Xist, Lox sites flanking exon 1,2,3 C57BL/6 Mouse line with conditional deletion of Xist Unpublished...

... 10 11,750 Mouse Cherry Brie Pooled Library 170511 Knockout Mouse Tolar 3rd ~4 78,637 Mouse Chromatin ...130,209 Mouse genome-wide library v1 Discontinued Knockout Mouse Yusa 3rd 5 87,897 Mouse improved genome-wide...Knockout Mouse Birsoy 3rd 8 22,909 Mouse Liver CRISPR Knockout Library 192824 Knockout Mouse Knouse 3rd...~5 71,878 Mouse messenger-RBP sgRNA Library 169082 Knockout Mouse Turner N/A 10 13,500 Mouse Metastasis...123361 Knockout Mouse Chen 3rd 4 11,934 arrays Mouse SLC Knockout Library 141436 Knockout Mouse Superti-Furga...Knockout Mouse Chen N/A (AAV) 4 286 Mouse Validation (mVAL) CRISPR Library 159391 Knockout Mouse Moffat ...Knockout Mouse Doench and Root 3rd 4 78,637 Broad GPP Humagne Set C and Humagne Set D 172650 (Set C) 172651...

...standard laboratory mouse. It’s also made targeted insertions relatively simple in C. elegans and bacteria...terminal); 2xNLS (C terminal) 47327 pET-28b-Cas9-His 6xHis (C terminal); NLS (C terminal) 62933...6xHis 6xHis (C terminal); NLS (C terminal) 62934 pET-NLS-Cas9-6xHis 6xHis (C terminal); NLS ...process of mouse model generation simpler, as described in an earlier post on generating CRISPR mouse models... mutation in mouse ES cells, select for the mutation and inject the ES cells into a mouse embryo to create...bacteria. But CRISPRing typical mouse models, including creating Cre-dependent conditional alleles, has remained...knock-in. Read on to learn how you can make CRISPR mouse model generation easi-er! Generating mice before...

...channel Mouse Mouse IgG2a 114542 Anti-LRP4 (cytoplasmic) [N295B/66R] LRP4 (cytoplasmic) Mouse Mouse IgG2a...Mitofusin-2 Mouse Mouse IgG2a 114548 Anti-Mortalin/GRP75 [N419/40R] Mortalin/GRP75 Mouse Mouse IgG2a 114549...Synaptotagmin-10 Mouse Mouse IgG2a 114559 Anti-Ataxin-1, 11NQ [N76/3R] Ataxin-1, 11NQ Mouse Mouse IgG2a 114560...SynCAM4 Mouse Mouse IgG2a 114563 Anti-TRIP8b (exon 1a/5) [N291C/22R] TRIP8b (exon 1a/5) Mouse Mouse IgG2a.../130R] Homer1L/S Mouse Mouse IgG2a 177457 Anti-Homer1L [L113/13R] Homer1L Mouse Mouse IgG2a 177458 Anti-Homer1L...[L131/17R] SPHKAP Mouse Mouse IgG2a 177478 Anti-SPHKAP [L131/20R] SPHKAP Mouse Mouse IgG2a 177479 Anti-SPHKAP...K+ channel Mouse Mouse IgG2a 177497 Anti-Thorase/Atad1 [N125/10R] Thorase/Atad1 Mouse Mouse IgG2a 177498...

...Tobey, I. G., Moncada-Reid, C., Eid, F.-E., Walkey, C. J., Ljungberg, M. C., Lagor, W. R., Heaney, J. ...viral yields relative to AAV9. At lower viral titers than AAV-PHP.B (e.g., 1 x 1011 vg/mouse), AAV-PHP.eB...Ravindra Kumar, S., Adams, C. D., Yang, D., Wang, T., Wolfe, D. A., Arokiaraj, C. M., Ngo, V., Campos, L....Gong, Y., Ng, C., Nammour, J., Maiz, D., Dujardin, S., Ramirez, S. H., Hudry, E., & Maguire, C. A. (2019)... Poterba, T., Boutros, C. L., Balazs, A. B., Daneman, R., Bloom, J. M., Seed, C., & Deverman, B. E. (2019...can also be paired with Cre-expressing transgenic mouse lines to restrict transgene expression to specific...systemic capsids express differently in different mouse strains, a phenomenon that we are calling “strain-specific...

...an ortholog in C. elegans making it ideal to study basic molecular biology processes. C. elegans are also...PMCID: PMC5525645. Mello, Craig C., et al. "Efficient gene transfer in C. elegans: extrachromosomal maintenance..., but there are many more out there. The house mouse (Mus musculus) Let’s start with the most preferred...mammalian model organism of many researchers: the mouse (Mus musculus). Mice have many advantages as a mammalian... model organism for scientists as they have a relatively short generation time for mammals - the time ...that are laid externally. Fruit flies are also relatively straightforward to manipulate genetically. Scientists...6 weeks compared to months to make a transgenic mouse line. Over the years the fly has become an ideal...

...HcKCR1 in mouse neurons. D) Membrane voltage traces of action potentials in a HcKCR1-expressing mouse neuron...EYFP and tdTomato expressed layer 2/3 neurons in mouse. C) Action potential frequencies with (magenta) and...recognized by reliable antibodies. It is derived from the C-terminal region of the human proto-oncogene c-Myc. ... on the Anti-c-Myc [9E10] page for more details. Image credit: Addgene.   Find anti-c-Myc antibody... as an optogenetic silencer in experiments with mouse cortical neurons, far out-performing previous attempts...potassium channelrhodopsins Myc-tag antibody anti-c-Myc [9E10] now available! Improved voltage indicator...10.1101/2021.09.17.460684.)   Myc-tag antibody anti-c-Myc [9E10] now available! by: Ashley Waldron The majority...

...reproducible rat or mouse rAbs. The other eight are chimeric antibodies composed of rat or mouse variable regions...alpha-5 Mouse IgG1 Rabbit Anti-Integrin alpha L [IPI-TS2/4] Integrin alpha-L Human IgG2a Mouse Anti-Integrin...Integrin alpha-L Mouse IgG2a Rat Anti-Integrin alpha M [IPI-M1/70] Integrin alpha-M Mouse, Human IgG2a...Human IgG2a Mouse Anti-Integrin alpha M [IPI-CBRM1/29] Integrin alpha-M Human IgG2a Mouse Anti-Integrin...alpha-M Human IgG2a Mouse Anti-Integrin alpha 5 [IPI-MFR5] Integrin alpha-5 Mouse IgG2a  Rat   ...IgG1) chimeric antibodies, and others are rat or mouse IgG2a. Additional human IgG1s are also available...function-blocking antibody, αVβ3.13, may also block function of mouse αVβ3, again the first known reagent to do so. The...

...the Allen Mouse Brain Connectivity Atlas, a project to map neuronal connections in the mouse brain. For...J.G., Wohnoutka, P., Gerfen, C.R., Koch, C., Bernard, A., Dang, C., Jones, A.R., and Zeng, H. (2014). A ..., J. M., Vandenberghe, L. H., Haute, C. V., Thiry, I., Deroose, C. M., Mortelmans, L., Wilson, J.M., Debyser...finemap of neurons in the mouse brain. MouseLight images can be found on the MouseLight website. Together with...overview see Addgene’s AAV guide. AAV particles are relatively small (20 nm) in size which makes them capable...fluorescent marker. Injection into a conditional mutant mouse brain that expressed Cre recombinase only in pyramidal...injected at several hundred sites across the entire mouse brain. Traced neurons expressed a fluorescent marker...

...sophistication of mouse transgenics. Everything that has been described is intended for the mouse; however, all...response gene c-fos. For example, using Chr2 stimulation should result in robust increases in c-fos expression...stimulation compared to controls without Chr2. c-Fos is a reliable and widely used molecular marker of neuronal...[3], hippocampal neurons are activated when the mouse is placed in a novel context. As a proof-of-principle...electrophysiology. Concluding thoughts and moving beyond the mouse While it sounds like a lot of steps and a lot of...standardization of genetic tools in the laboratory mouse, most optogenetics is performed in this species....proceed with your behavioral experiments. Comparison of c-fos amongst animals that receive different types of...

...Cre (CT#644) Cre; expression in developing limb mouse Prx1 Bacterial Tabin 14796 pClusterin-ERT2CreERT2... construct CMV Mammalian Hughes 40591 hGFAP-Cre mouse astrocyte expression of Cre hGFAP Mammalian Messing...fusion; Tet inducible - rrTA expression driven by mouse Nkx cardiac enhancer and promoter fragment minimal...Cre-ERT2 - Tamoxifen inducible; Targeting vector Mouse Nxph4 Mammalian Zeng 62372 pET-(-30)GFP-9xGGS-Cre...inducible. Expressed in limb connective tissue mouse Prrx1 Vertebrate Tanaka 111151 Col1A2:TFPnls-T2a-mErt-Cre-Ert... Tamoxifen inducible. Expressed in fibroblasts mouse Col1A2 Vertebrate Tanaka 111152 Col2a1:TFPnls-mErt-Cre-Ert...Eisenhoffer 131781 pCS2+PhyBΔCreC CreLite - Red light-inducible Cre; C terminus Cre fused to PhyBdelta ...

...cysteine) pTP955 (3x cysteines) Mouse IgG1 TP886 pTP943 (3x cysteines) Mouse IgG1 TP1107 pTP1112 (1x cysteine... TP1122 (3x cysteines) Mouse IgG2a TP1129 pTP1005 (3x cysteines) Mouse Kappa TP1170 pTP1174 (1x ...require secondary antibodies, frequently of the mouse or rabbit variety. While antibodies certainly aren...immunodetection? Enter the Görlich lab and their anti-mouse and -rabbit IgG secondary nanobodies toolbox. Nanobodies... Monovalent Monoclonal Only anti- rabbit and -mouse versions are currently available Not commercially...screening, optimizing, and characterizing several anti-mouse and -rabbit nanobodies. If you’re interested, you...Bates, M., & Görlich, D. (2017). A toolbox of anti–mouse and anti–rabbit IgG secondary nanobodies. The Journal...

...other C-terminal antibodies only recognized the human c-Myc protein and did not recognize the mouse or chicken...bound to the C-terminal peptide. One of these C-terminal antibodies was Myc1-9E10 (aka anti-c-Myc [9E10]...rat IgG1, human IgG2, mouse IgE, goat IgG, (and more!) as well as the original mouse IgG1. And the refinement...sequences within the middle of the human c-Myc protein or the C-terminus, Evan, et al. isolated six different...experiments? Why Myc? When did the commonly used anti-c-Myc [9E10] antibody come into play? We’ll dive into...This protein had a highly specific antibody to the C-terminal five amino acids, meaning the tag could be...However, substance P has an amide group added to its C-terminus post-translationally, which turned out to...

...Example: ‘h’ is for Human (homo sapiens), ‘m’ is for mouse (mus musculus), ‘r’ is for rat (rattus rattus or...the order they appear in the plasmid and their relative position to the insert. Example, if you have a... immediately followed by its position (number) relative to the start Methionine (Met) followed by the ...resistance, b) the promoter that drives the insert, and c) any other selection markers (for use in other cell...pBACKBONE-Flag-hGene If there was also an EGFP fused to the C-terminal of your insert you would list it after the...

...work should be done in mouse and large animal models with mutations in human-relevant exons 45-55, rather...CRISPR can treat a genetic disease in a postnatal mouse model, an important proof of concept for future ... when the genome editing frequency is low, the relative abundance of exon-skipped mRNA is high, likely...rather than in the traditional exon 23-mutated mouse model. Potential off-target effects in muscle, as well...editing partially restores dystrophin expression in a mouse model of muscular dystrophy. Science. 2015 Dec 31...vivo genome editing improves muscle function in a mouse model of Duchenne muscular dystrophy. Science. 2015..., Wagers AJ. In vivo gene editing in dystrophic mouse muscle and muscle stem cells. Science. 2015 Dec ...

...optimum of 30 °C and is therefore inefficient in mammalian cells (usually grown at 37 °C). Smart molecular...of Cre recombinase are used in combination with mouse lines that express Cre only in the cell type of ...neurons and their synapses in a conditional knockout mouse. Tamoxifen injection induces Cre-ERT2 recombinase... before and after the gene knockout in the same mouse.   Scientists always strive for more. In order...gene and expression of a separate gene. In these mouse lines, gene knockout can be induced by Cre recombinase...Kranz, Andrea et al. 2010. “An Improved FLP Deleter Mouse in C57Bl/6 Based on FLPo Recombinase.” Genesis 48...Cre-Mediated Recombination at the Cellular Level in the Mouse.” Nature Biotechnology 21(5): 562–65. PubMed PMID...

...: 95 °C for 15 min, 35 cycles of (95 °C for 30 sec, 60 °C for 1 min, 72 °C for 1 min), and 72 °C for 10...following parameters: 37 °C for 30 min; 95 °C for 5 min and then ramp down to 25 °C at 5 °C/min. Dilute oligos...parameters: Cycles 1-20 (37 °C for 5 min, 20 °C for 5 min); Cycle 21 (80 °C for 20 min). These cycling ...Incubate at 30 - 37 °C for 24 - 72 hr. 30 °C may enhance genome editing efficiency, but 37 °C is acceptable....cells to incubate at 37 °C for 3 - 7 days and allow the clones to incubate at 37 °C for 7 - 14 days. Vary...thermocycler and run the following program: 65 °C for 6 min, 98 °C for 2 min to extract gDNA. Measure the DNA...clones. Run sample in thermocycler: 65 °C for 6 min and 98 °C for 2 min to extract gDNA. Screen each clone...

...lentiviral CRISPR library from Alec Kimmelman targets mouse metabolic genes with ~6 gRNAs per gene.  CRISPR-...activity on NAG PAMS and reduced activity on NGG PAMs relative to wild type. A new genome-wide CRISPR knockout...library from Xiaole Shirley Liu's lab targets all mouse genes with 10 gRNAs per gene. The guides are optimized...) Strategy for leak-free transcriptional control. C) Iterative transduction workflow overview. Image from...nucleases identified, functions at temperatures up to 43 °C and can be used for genome editing in moderate thermophiles...opportunities for investigating genetic mechanisms related to morphogenesis, cell fate specification, and ...the internal control (Fig 1). Because this method relies on the fluorescence ratio between the 2 fluorophores...

...Additional resources on the Addgene blog Mouse Modeling Part 1 Mouse Modeling Part 2 Five Popular Model Organisms...(Ed.), The Laboratory Mouse. Academic Press. Turner, P. V., Brabb, T., Pekow, C., & Vasbinder, M. A. (... P., Rodriguez-Aguayo, C., Walton, B. L., Sood, A. K., Greaver, J., Janssen, C. F., & Lopez-Berestein,...through the proper basic training prior to conducting mouse experiments. Some of these techniques are what we... to pick up with minimal practice after initial mouse training. Other techniques require more skill and...ingestion, such as experimental therapies or pain relief, straight to the stomach. This technique involves...