We narrowed to 8 results for: pSAM

... results (Goutaudier et al., 2019). Using PSAMs PSAMs PSAMs, or pharmacologically selective actuator modules...working with PSAM receptors, your impact on the cell is determined by what IPD your PSAM is combined with... will promote hyperpolarization. PSAM ligands The advantage of PSAMs lies in their ability to be specifically...currently used in chemogenetics are DREADDs and PSAMs. Using DREADDs DREADDs DREADDS, or designer receptors...activation and reduced off target activation by ligands. PSAMs themselves are modified ligand binding domains. ...coupled to the ion pore domain (IPD) of a channel, the PSAM acts as a gating mechanism to control ion flux. ... with. Combining a PSAM with the IPD of a cation channel, such as the serotonin 3 receptor, will promote...

...PSEMs). PSAMs are coupled to an ion pore domain to create a ligand-gated ion channel. Whether a PSAM is activating...chemogenetic experiments, including popular DREADDs and PSAMs. Plasmid...and Pharmacologically Selective Actuator Modules (PSAMs). DREADDs are the most widely used chemogenetic ...activating, while hM4D(Gi) and KORD are inhibitory. PSAMs are engineered α7 nicotinic acetylcholine receptor...it is coupled to. Addgene has two main types of PSAMs: 5HT3 coupled, which are activating, and GlyR-coupled...

...localization. The Sternson lab recently deposited PSAM AAV, which encode engineered ligand-gated ion channels...serotype testing jGCaMP7c for calcium detection PSAM4 GlyR for chemogenetic inhibition Flp expression ...Plasmid Serotype Name 119741  AAV5  AAV SYN flex PSAM4 GlyR IRES EGFP Recombinase AAV Finally, we are... Plasmid Serotype Name 119472  AAV5  AV SYN PSAM4 GlyR IRES EGFP   Biosensors Plasmid Serotype...

...signaling. PSAM-based LGICs can be classified by the ion pore domain they are coupled to: PSAM-Gly PSAM-Gly ...and inhibits neuronal activity. PSAM-5HT3 PSAM-5HT3 LGICs pair a PSAM domain with a 5-HT 3 serotonin receptor...varenicline. This PSAM carries mutations at L131G, Q139L, and Y217F and is termed PSAM4 (or PSAM 4 ). Additional...arePharmacologically Selective Actuator Modules (PSAMs, pronounced SAMs). PSAMs are engineered α7 nicotinic acetylcholine...cognate PSEM. For example, PSAM Q79G,Q139G is activated by PSEM 22S , while PSAM L141F,Y115F is activated... effort to develop a PSAM that responded to a clinically approved drug, the PSAM domain was engineered...neurons. Table 2: Descriptions of PSAMs and their activity in neurons PSAM Ion Pore Domain Ligand(s) Effect...

...Service overview. Function Chemogenetics DREADDs, PSAMs Optogenetics Channelrhodopsins, Halorhodopsins, ...

...flex PSAM4 GlyR IRES EGFP Chimeric channels for neuronal manipulation 119742  AAV5  AAV SYN PSAM4 GlyR...

... rg* Ulrik Gether 119741 AAV SYN flex PSAM4 GlyR IRES EGFP PSAM4 GlyR - Inhibition IRES EGFP Cre-dependent...Cre-dependent 5, 9 Scott Sternson 119742 AAV SYN PSAM4 GlyR IRES EGFP PSAM4 GlyR - Inhibition IRES EGFP none 5 Scott...Scott Sternson 119744 AAV CAMKII PSAM4 GlyR IRES EGFP PSAM4 GlyR - Inhibition IRES EGFP none 5 Scott Sternson...Inhibitory HCAD(Gi) DREADD hM4D(Gi) DREADD KOR DREADD PSAM4 GlyR Promoter Synapsin CaMKIIa CD68 Dlx GFAP nEF...

...eYFP Viviana Gradinaru 119741-AAV5 AAV SYN flex PSAM4 GlyR IRES EGFP Chemogenetics Cre-dependent expression...plasmid Scott Sternson 119741-AAV9 AAV SYN flex PSAM4 GlyR IRES EGFP Chemogenetics Cre-dependent expression...expression plasmid Scott Sternson 119742-AAV5 AAV SYN PSAM4 GlyR IRES EGFP Chemogenetics expression plasmid ...plasmid Scott Sternson 119744-AAV5 AAV CAMKII PSAM4 GlyR IRES EGFP Chemogenetics expression plasmid Scott Sternson...