RNA Interference in Plant Biology: New Tools for an Old Favorite
Type
Blog Post
... analogs such as 2-fluoroadenine (2-FA), organisms with functional APT will convert 2-FA to cytotoxic ...selection of plants undergoing RNAi, and LIIbeta F 1-2 RNAi, which allows assembly of intron-spliced hpRNAs...nucleotides, resulting in death. In the presence of 2-FA, potent gene silencing of APT is required for survival...block of our understanding of complex biological processes. Many tools exist to perturb biological function... interference (RNAi) is a conserved eukaryotic process where approximately 20-30 nucleotides of double-stranded...formation of a hairpin RNA structure, resulting in processing by the endogenous RNAi machinery. Alternatively... engineered to created artificial miRNAs. The processed miRNA or siRNAs then silence the target gene(s...