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Showing: 21 - 40 of 66 results
  1. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ..., reagentless biosensor for ADP based on tetramethylrhodamine-labeled ParM A fluorescent, reagentless ...reagentless biosensor for ADP based on tetramethylrhodamine-labeled ParM. ACS Chem Biol. 2010 Apr 16;5(4):415-25...reporter VARNAM Sensitivity optimization of a rhodopsin-based fluorescent voltage indicator. Neuron. 2023... mammalian neurons using engineered microbial rhodopsins. Nat Methods. 2014 Aug;11(8):825-33. Adam Cohen...D95N), MacQ-mCitrine, QuasAr and QuasAr-mOrange) Rhodopsin-based voltage imaging tools for use in muscles...
  2. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    ...metagenomically discovered marine anion-conducting channelrhodopsin used as a sensitive fluorescent voltage indicator...Hyperpolarizing chloride channel SwiChr++ - A channelrhodopsin converted into a chloride-conducting anion... step-function artificial anion conducting channelrhodopsin (aACR). It is activated by cyan light and ...
  3. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    ...Ace-mScarlet, a fusion of a voltage-sensitive inhibitory rhodopsin (Ace2N) with the red fluorescent protein (mScarlet...fusion between the voltage-sensitive inhibitory rhodopsin Ace2N and mScarlet. Ace2N quenches a proportion...green-fluorescent GEVIs and blue-light-sensitive channelrhodopsins. Find the Ace-mScarlet voltage indicator plasmids...
  4. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...Patrick Miller-Rhodes from the University of Rochester Medical Center. Patrick Miller-Rhodes is currently...This post was contributed by Patrick Miller-Rhodes, a Ruth L. Kirschstein NRSA Predoctoral Fellow at ...
  5. EXPRESs Plasmids for Plasmodium falciparum

    Type
    Collection
    ...47778 RH2b-bio 47779 RH4-bio 47780 RH5-bio 47781 RhopH3-bio 47782 SPATR-bio 47783 AARP-bio 47784 Pf34-bio...RAP2-bio 47796 RAP3-bio 47797 CLAG3.2-bio 47798 RhopH2-bio 47799 RON3-bio 50737 RAMA-bio-His 50800 Prohibitin-bio-His...
  6. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    ... illustrated with my favorite microorganism (Rhodobacter sphaeroides!) at the ASM booth. As well as being...Lakes Bioenergy Research Center and a fellow Rhodobacter enthusiast, Tim also hosted me at his University...
  7. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ..., Adam Cohen's lab presented Archaerhodopsin 3 (Arch) as a new rhodopsin based voltage indicator for use...plasma membrane. To obtain a more sensitive channelrhodopsin actuator for triggering action potentials ...lab further modified sdChr- a blue-shifted channelrhodpson from freshwater green alga identified by Ed...
  8. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    ... by delivering an AAV construct expressing channelrhodopsin 2 (ChR2), a blue light sensitive cation channel... Plasmid tools for microbiome studies Channel rhodopsins for optogenetics Viral Vectors 101: Chemogenetics...
  9. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...AAV-flex-taCasp3-TEVp here The red-shifted channelrhodopsin (C1V1) targeting parvalbumin cortical interneurons...use viral prep in serotype AAV5.  Get the channelrhodopsin here Astrocyte specific tight affinity glutamate...
  10. Optogenetics AAV Preps

    Type
    Collection
    ...optogenetics AAV collection, including channelrhodopsins, halorhodopsins, and more....
  11. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...Activation of these neurons via the channel Channelrhodopsin 2 (ChR2) reduced glutamate transmission from...mouse to deliver Cre-dependent expression of  Channelrhodopsin 2 (ChR2), a modified receptor protein that...
  12. Brain Initiative Collection

    Type
    Collection
    ...AAV9 pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST Cation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore and ...pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3 Cation channelrhodopsin ChroME targeted to the neuronal soma and proximal...
  13. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...Veenman et al., 1992) or fluorescent molecules like rhodamine-isothiocyanate (RITC; Thanos et al., 1987) are...Vidal-Sanz M., and Aguayo A.J. (1987). The use of rhodamine-B-isothiocyanate (RITC) as an anterograde and ...
  14. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...simultaneously measuring the activities of CaMKII and RhoA during induction of structural plasticity in single...expression plasmids, FRET constructs, and CaMKII and RhoA sensors. Laviv, et al. Nat Methods. 2016. PubMed...
  15. Newly Updated AAV Data Hub!

    Type
    Blog Post
    ...report describes a functional vector such as a channelrhodopsin Images showing the injection site as well ...
Showing: 21 - 40 of 66 results