...research from the O'Connor-Giles, Wildonger, and Harrison labs.... Protocols gRNA Design Tools CRISPR Blog Posts Harrison Lab - Wildonger Lab - O'Connor-Giles Lab email..., Cummings AM, Nguyen JN, Hamm DC, Donohue LK, Harrison MM, Wildonger J, O'Connor-Giles KM. Genetics ....

..., 9 Sternson 119742 AAV SYN PSAM4 GlyR IRES EGFP PSAM4 GlyR - Inhibition IRES EGFP none 5 Sternson 119744... EGFP PSAM4 GlyR - Inhibition IRES EGFP none 5 Sternson 121538 pAAV SYN1 HA-hM4D(Gi) hM4D(Gi) - Inhibition...

...standard toolbox of genetic methods, such as Tol2 transposons, Cre-mediated site-specific recombination, morpholinos... expression system for use in zebrafish - Bret Pearson Lab MAZe: a tool for mosaic analysis of gene function...digital spectral barcodes for cell tracing - Sean Megason Lab Zebrabow - Albert Pan and Alex Schier Labs....

...cells. Yao F, Svensjö T, Winkler T, Lu M, Eriksson C, Eriksson E. Hum Gene Ther . 1998 Sep 1;9(13):1939...target rtTA On Gu 60495 pSBtet-GP Sleeping Beauty transposon system; has luciferase in cloning site; see article...

... to know. In this module, you will learn about personal protective equipment (PPE) and general laboratory...

... 1, 9 GENIE 174007 pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST Syn jGCaMP8s none Cre-dependent 9 Histed 176749...pENN.AAV.CamKII.GCaMP6f.WPRE.SV40 CamKII GCaMP6f none Constitutive 1, 5, 9 Wilson 100835 pAAV.CAG.Flex.GCaMP6f.WPRE.SV40 CAG GCaMP6f...AAV.CamKII.GCaMP6s.WPRE.SV40 CamKII GCaMP6f none Constitutive 9 Wilson 111262 pAAV-hSynapsin1-axon-GCaMP6s Syn axon-GCaMP6s...

...Rudolf Jaenisch Keisuke Kaji Jonathan Katz Nathan Lawson Kathrin Plath Miguel Ramalho-Santos Derrick Rossi...Rossi Michael Ruppert Michel Sadelain James Thomson Tim Townes Robert Weinberg Knut Woltjen Shinya Yamanaka...

...650 13.4 pcDNA3-mNeptune-N E2-Crimson 611/646 28.98 pEF.myc.ER-E2-Crimson Near-Infrared Protein Excitation...

...of how to weigh reagents. Wear the appropriate personal protective equipment (PPE) prior to handling the...

...Plasmid Description PI Publications Kaufman ML, Goodson NB, Park KU, Schwanke M, Office E, Schneider SR...10.1242/dev.199399. Epub 2021 Jun 18. PMID 34143204 Goodson NB, Kaufman MA, Park KU, Brzezinski JA 4th Development...

...CRISPR tools Fluorescent Proteins from the Michael Davidson collection Protein Labeling from New England Biolabs...RESOLUTE collection AAV Packaging from Addgene Parkinson's Research from The Michael J Fox Foundation Search...

...key strategies of aseptic technique, including personal protective equipment, setting up a clean workspace...

...Centrifuge Microfuge tubes or other suitable container Personal protective equipment (PPE) appropriate for the...

...Engineering SynBio Depositing Labs Hal Alper J. Chris Anderson Adam Arkin Gabor Balazsi Matthew Bennett Carolyn...SynBio Deposits Alper Lab Y. Lipolytica Promoters Anderson Lab Plasmids and Phagemids Balazsi Lab Negative...

...Transfection none, need Cas9 plasmid O'Connor-Giles, Harrison, Wildonger pU6-BbsI-chiRNA dU6 BbsI Transfection...Transfection none, need Cas9 plasmid O'Connor-Giles, Harrison, Wildonger Do you have suggestions for other plasmids...

...Star Activity" and can happen for a variety of reasons, including high glycerol concentration. Learn more...

...Right after entering the lab, put on the proper personal protective equipment (PPE) and wear it the whole...

...Sequence-Specific Control of Gene Expression. Qi LS, Larson MH, Gilbert LA, Doudna JA, Weissman JS, Arkin AP...Regulation of Transcription in Eukaryotes. Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, Stern-...

...diabetes. Kissler S, Stern P, Takahashi K, Hunter K, Peterson LB, Wicker LS. Nat. Genet. 2006. Apr;38(4):479...cells and transgenic mice by RNA interference. Rubinson DA, Dillon CP, Kwiatkowski AV, Sievers C, Yang...

...Casilio system from Albert Cheng's lab at Jackson Laboratories. Next generation CRISPR-Cas platform for...Background The Albert Cheng and Haoyi Wang labs at The Jackson Laboratory (JAX) have developed a CRISPR/Cas platform...