...neuronal connectivity. These methods are less neurotoxic and better compatible with other neuroscience...system where it replicates, spreads, causes neurotoxicity, and is lethal if left untreated (for review...they also have low immunogenicity and cellular toxicity. AAV vectors can be equipped with strong promoters...while also having the added advantage of lower toxicity, higher specificity and reduced technical variability...Wang, Y., and Harvey, B.K. (2008). Tropism and toxicity of adeno-associated viral vector serotypes 1,2,5,6,7,8,9...retrograde transsynaptic transfer of a protein, tetanus toxin, subsequent to its retrograde axonal transport. ...

...enough signal. This workaround risks cellular phototoxicity and eliminates the possibility of long-term ...have the potential to form aggregates and may be toxic to the cell. Here comes the SunTag How does SunTag...bright images of mitochondria without organelle toxicity. First generation (v1) SunTag is expressed at...brightness and signal-to-noise ratio Less chance of phototoxicity Reduced photobleaching Simplified, long-term...

... If a drug is equally toxic to both lines, it probably has either high toxicity or high off target effects... to no toxicity in the knockout line, it is likely a specific drug with low off-target toxicity. This ...

...tagRFP Ebert pSAG1::CAS9-U6::sgUPRT 54467 Other/Toxoplasma gondii none, Q5 mutagenesis yes, cut S. pyogenes...pyogenes Sibley pU6-Universal 52694 Other/Toxoplasma gondii BsaI yes, cut S. pyogenes Lourido pRGE31 50929...

...eliminate the competition via the production of toxins and antibiotics. Fungi such as Penicillium produce...penicillin, and bacteria in turn possess plasmid-borne toxins like colicins and subtilisins to kill other bacteria... carrying genes for the production of bacterial toxins like colicin also often carry the necessary resistance...organisms can’t use. Others develop resistance to toxic substances such as heavy metals or pesticides, enabling...

...which can be toxic in living cells. BioID was derived from biotin ligase and uses far less toxic biotin for...pair of enzymes that overcome both the speed and toxicity limitations of APEX2 and BioID. In the Ting Lab...describe TurboID and miniTurbo, which utilize non-toxic biotin, but label nearly as quickly as APEX2. What...

...2 plasmid) Knockout Human Moffat 3rd 4 70,948 Toxoplasma Knockout 80636 Knockout T. gondii Lourido N/A...

...important to note that the biotin-phenol reagent can be toxic to living cells thus APEX is not suitable for organoid...APEX2 plasmids! TurboID and minturbo: Rapid and non-toxic proximity labeling  TurboID and miniTurbo were developed...rapidly label proteins, requires H2O2 which can be toxic to living cells. To circumvent these issues, the...biotin labeling in 10 minutes without any issues in toxicity. TurboID’s signal is significantly greater than...

...recognized by effector cells of the immune system - cytotoxic T lymphocytes (CTLs), natural killer (NK) cells...programmed cell death ligand (PD-L1) that inhibits the cytotoxic activity of CTLs and NK cells. These cells would...immunotherapy. Using activated DCs ex vivo to generate cytotoxic effector T cells, and targeting specific DC subsets... targets like CD19 on tumor cells and exhibit cytotoxic activity. CAR-T cells have consistently shown ...

...live-cell imaging, the use of dyes that exhibit low toxicity and high photostability is crucial. These properties...dye's ability to enter cells without causing undue toxicity. A prominent example is DAPI, a dye that binds...in live cells can sometimes result in cellular toxicity due to the permeation process. Advanced technologies...of fluorescently tagged nanocellulose for nanotoxicological studies. Environmental Science: Nano, 6(5)...

...transduction) targeting a gene associated with diphtheria toxin lethality and were sorted into single wells to generate...cells showed up to 70% resistance to diphtheria toxin, indicating a 70% frequency of biallelic mutations... by nature transient, which may lower nuclease toxicity or the potential for off-target effects. Third...

...replication in mammalian cells, for example, and a toxin that kills bacteria may not have any discernable... a cassette that will make cells resistant to a toxin), whereas negative selection would be the picking... a cassette that will make cells sensitive to a toxin). In the table below we focus on positive selection...

...Long-term light exposure can also lead to cellular phototoxicity, and experimental success can be affected by...-lanterns are not subject to photobleaching/phototoxicity, they successfully imaged multiple subcellular...imaging, especially where photobleaching and phototoxicity are a concern, these constructs come in very...

...core of this tool is the endoprotease Botulinum Neurotoxin B (BoNT/B), which cleaves the synaptic protein... to SspB, brings together the split botulinum neurotoxin halves to enable VAMP2 cleavage, and suppresses...photodimerizer system CRY2-CIB1 to regulate botulinum neurotoxin were also created. The depositors demonstrated...

...starting knock-in experiments. Test dTAG molecule toxicity in new cell lines Treat parental cells (or cells...biological assay of choice to ensure that there are no toxicities at the doses used to evaluate degradation of ...have evaluated to date, we observed little to no toxicity with dTAG-13 at doses as high as 20 µM (see Figures...

... blue light (Bubeck et al., 2018). 3. Reduce cytotoxicity of CRISPR-Cas editing in tissues The small size... systems prevents the off-target effects and cytotoxicity that result from excessive nuclease activity...Expressing Anti-CRISPR Peptides Decreases CRISPR/Cas9 Toxicity in Human Hematopoietic Stem Cells. Molecular Therapy...

... cool things about amp is that it's exclusively toxic to dividing cells. The other antibiotics listed ...across systems and species. The cost? Zeocin is genotoxic when not 100% inhibited, meaning it will induce...there is evidence that zeocin is never entirely detoxified, even when Sh ble is expressed (Trastoy et al...

...signs of toxicity or burden relative to a fluorescent protein control. We also added the toxin-antitoxin...

...genetic tools to the brain because of its low cytotoxicity, low immunogenicity, and the long-term expression...of FPs can trigger protein aggregation which is toxic for the cells and affects the labeling of small ...fluorescence. Furthermore, many of these FPs are cytotoxic, prone to aggregation and cross-react with anti-GFP...

...antigens found in the body or self antigens, and toxins that are lethal to animals.  In the clinic, scFvs...tumor site. ScFvs can also be fused to cellular toxins, radioisotopes, cytokines, and enzymes for cancer...facilitates signal transduction and release of cytotoxic granules from the T cell. When a CAR has an scFv...