...severing and has very low dark-state activity and toxicity. All of that means this light-powered sword truly... 30x SuperPLDs avoid dark-state activity and cytotoxicity better than the 50x and 100x variants. But those...great for in vitro biocatalytic applications where toxicity doesn’t matter. Plus, for folks interested in ...

...DNA sequence and has been suggested to cause cytotoxicity [5]. Second, instead of using a donor-plasmid...plasmids in some cell lines, most likely due to DNA toxicity. More importantly, unlike Cre recombinase, the...“Recombination between inverted loxP sites is cytotoxic for proliferating cells and provides a simple ...

...fluorescence. Since emission of green light is more phototoxic to cells, most scientists working on in vivo ...fluorescence when exposed to blue light, which is less phototoxic to live tissue than the UV light used by other... a reduced potential for photobleaching and phototoxicity that standard FPs might induce though their ...

...Photo-activatable iRFPs can be ‘turned on’ by non-phototoxic far-red light and are used for spatially selective...

...Cre-dependent Cre-dependent expression of diphtheria toxin receptor fused to GFP for studying cell ablation...

...apicomplexan organism Toxoplasma gondii to the infection of human fibroblasts. Toxoplasma mutants were generated...Mülleder M, et al. F1000Res. PubMed PMID: 27830062 Toxoplasma CRISPR knockout pooled library Parasites from... and livestock diseases such as malaria and toxoplasmosis. Despite their importance to global health, ...

...of DNA into 500 ul of Opti-Pro SFM. *Pro-Tip* Endotoxins can inhibit transfection, therefore, plasmid ...plasmid DNA purification should include an endotoxin removal step. For high quality plasmid DNA, the plasmid...

...these DNA samples must also be entirely endotoxin free. Endotoxin is a lipopolysaccharide of the cell wall...

...and auxotrophy) and negative selection (ex: toxin-antitoxin systems and sacB counter-selection) tools used...

...optimized for each transfer plasmid. *Pro-Tip* Endotoxins can inhibit transfection; therefore, plasmid ...plasmid DNA purification should include an endotoxin removal step. For high quality plasmid DNA, the plasmids...

...Entamoeba histolytica Leishmania sp. Plasmodium sp. Toxoplasma gondii Trypanosoma sp. Plasmids for Viruses Species...

...of Pharmacology and Toxicology 55:399–417 . https://doi.org/10.1146/annurev-pharmtox-010814-124803 Vlasov...

...want to make a lot of your plasmid? Is the gene toxic in high amounts? It is always good to keep in mind...they contain large inserts or genes that create a toxic product. The E. coli strain Most E. coli strains...

...using this plasmid Cas9 expression is typically cytotoxic, which makes generation of high titer Cas9 lentiviruses...lentiviruses challenging. This cytotoxicity also causes growth rate variability when using lentiCas9-blast...

... cell toxicity. Cas7-11 does not display collateral RNA cleavage and is significantly less toxic to mammalian...

...spatial precision of gene expression and reduces toxicity as compared to previous Cre/lox or drug-induced...inactivating Cre. This is critical for reducing Cre toxicity in long-term experiments.  Figure 4: The...

...demonstrated that it can also detect expression of toxin mRNA from Clostridium difficile. As mRNA expression...methods, the ability to detect mRNA transcripts of toxins and other transcripts of interest is a benefit ...

...advancement over previous chemical versions with toxicity to many organisms. Fig. 1: Exogenous sources... to local dsRNA production. In the same vein, genotoxic stress, such as chemotherapy treatment or ionizing...

...cells remain difficult to genetically edit due to toxicity from electroporation or poor cellular entry with...frequency (nearly 100% in some cases) and minimal toxicity, including in clinically-relevant lines such as...

...extremely useful if a desired protein product is toxic to cells at high concentrations. Despite their advantages...lower expression may be required if your protein is toxic to E. coli. Of all the vectors, pET, under the control...