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We narrowed to 223 results for: his-tag

Showing: 1 - 10 of 223 results

  1. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...purification based on the polyhistidine tag. What is the polyhistidine tag? The polyhistidine tag is a string of ...Benefits of HIS tags In addition to providing a way to purify a protein of interest, polyhisitidine tags can ...low level, try a longer histidine tag to increase capture. Longer histidine tags are also recommended if... isolated.   Prior to the polyhistidine tag, alternative fusion tags were used, such as S. aureus’... conditions.   Find polyhistidine tag plasmids here! Where should I place the tag and how many do I need...plasmid containing a polyhistidine tag, you’ll need to decide whether to add the tag to the N- or C-terminus... however, tag placement can affect protein function. B, The string of histidines in the tag is typically...

  2. Antibodies 101: Affinity Tags

    Type
    Blog Post
    Published
    Sept. 19, 2023, 1 p.m.
    ...covered! His His tags, often called polyhistidine tags, typically include six consecutive His residues...Diagram of His-tagged protein purification work flow by nickel column.    Affinity tags vs. other ...other tags There are many different types of tags, including epitope tags (FLAG, Myc, etc.) and fluorescent...blog Plasmids 101: Protein tags Antibodies 101: Epitope tags To Each HIS Own ...planning, affinity tags can make protein purification a cinch. Types of affinity tags, the proteins they...are all reviewed here. What are affinity tags? Affinity tags are used primarily as protein purification... common tags available. Maltose binding protein Maltose binding protein (MBP) is a 45 kDa tag which can...

  3. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...affinity tag is the polyhistidine (His) tag. Small and unlikely to affect function, His-tagged proteins...usually using a Ni2+ column. Like other affinity tags, a His tag can be fused to either the N- or C-terminus...Combo and cleavage tags Frequently, a single tag is not enough. What if you need one tag to increase solubility... to a purification scheme, His tags are frequently combined with other tags for dual-labeling. The problem...to all tags, but rather a quick overview of why scientists use tags, with a few time-tested tags and fusion...length a polyhistidine tract does not greatly alter the size of the tag.   Table 1: Common protein tags ...keep your protein soluble.  Tags for affinity and purification An affinity tag, generally a relatively small...

  4. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

    Type
    Blog Post
    Published
    July 29, 2014, 6:16 p.m.
    ...Tallahassee Democrat in a story about his life’s work and the stage IV lung cancer that has now prompted...State University has been the go-to place. In 2012, his National High Magnetic Field Lab worked with an impressive... all those collaborations over the years, he and his colleagues built a Molecular Expressions collection...spectra. A comprehensive list of cell markers all tagged with mEmerald. Plasmids to monitor protein-protein...available targets since the Davidson lab always tagged new proteins with mEmerald first, Baird says. From...cell membrane, this collection has fluorescently tagged versions of many proteins known to localize to ...prompted him to deposit the tools he and his team created with the research community at large, "[Fluorescent...

  5. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...which can be carried out using Addgene plasmids. His-tag labeling reagents You may already have a FRET acceptor-binding...for protein purification: the NTA binds to the His tag on your protein and the coupled Cy fluorophore ...C-terminal fusion of a tetracysteine tag. In addition, since these tags are so small, one can insert them... between 80% and 10%. C Cy3NTA and Cy5NTA bind His tags similarly but with differing R0 values, thereby...interest: you just don’t know it yet! The poly-histidine tag often used for protein purification is a reliable..., as well as by extending the length of the histidine tag from 6 to 10 residues. While these Cy/NTA conjugates...incorporation of fluorescent probes into protein: hexahistidine-tag-mediated fluorescent labeling with (Ni2+: ...

  6. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    Published
    Feb. 9, 2023, 2:15 p.m.
    ...same kinds of opportunities for his students. But he also found that his teaching responsibilities left...limited time for one-on-one mentoring. His solution? Bringing his research into the classroom. I had the...to hear about how he developed his program. Initially, Provost made his labs completely open-ended, allowing...bacteria, purified them to near homogeneity using a 6XHis tag, and compared their specific activities by measuring...purification and characterization of a fusion protein: Histidine tag, malate dehydrogenase, and green fluorescent...San Diego), decided to take a different approach. His own most impactful learning experiences happened ... opportunity to talk with Provost recently about his development of what is now commonly termed a Course-based...

  7. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type
    Blog Post
    Published
    June 26, 2018, 1:33 p.m.
    ...Campus in Aurora, Colorado, USA. The primary focus of his lab’s research is the mechanisms by which proteins...Gronostajski is a professor at the University of Buffalo. His research focuses on understanding the structural ...research on diseases of glycosylation, arginine mutagenesis, and neural development. Paula Videira: Congenital...as reporters of NFIX function. They will then mutagenize the NFIX gene and test whether variants of unknown...

  8. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ...optimized CyPet pCyPet-His Cyan Bacterial Expresses CyPet with C-terminal His tag SCFP3A Cyan Mammalian...optimized YPet pYPet-His Yellow Bacterial Expresses YPet with C-terminal His tag SYFP2 Yellow Mammalian...Bacterial Expresses Aquamarine with N-terminal His tag pAquaN1 Cyan Mammalian Expresses mammalian optimized...interactions in living cells using a microscope. Historically, molecular interactions were detected by indirect...

  9. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    Published
    Jan. 23, 2014, 3:23 p.m.
    ...promoters, N-terminal tags, coding sequences of the gene of interest, C-terminal tags, plant terminators ...science lab.” Introducing GreenGate As Lohmann and his colleagues describe in the PLoS ONE paper that introduced... it no longer works, just trash it.” Lohmann and his colleagues including Joachim Forner now expect and...aptly named GreenGate – offers all of those same advantages to researchers working in plant model systems...

  10. #DeckTheLab to Win a Tasty Winter Treat This Holiday Season

    Type
    Blog Post
    Published
    Nov. 30, 2015, 3:30 p.m.
    ..., too! Bluegene is eager to decorate the lab but his short arms are causing him some difficulty… To give...celebrating the holidays and tweeting it with the tags #DeckTheLab and @Addgene. Christmas, Hanukkah, ugly...you name it, we want to see it.      All photos tagged with #DeckTheLab throughout the month of December...
Showing: 1 - 10 of 223 results