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...Obstet Gynaecol, 2004. 18(6): p. 827-42. PubMed PMID: 15582541. 5. Seale, P., A. Asakura, and M.A. Rudnicki...Medicine. Geburtshilfe Frauenheilkd, 2015. 75(12): p. 1239-1242. PubMed PMID: 26726264. PubMed Central ...pluripotency and nuclear reprogramming. Cell, 2008. 132(4): p. 567-82. PubMed PMID: 18295576. PubMed Central PMCID...reprogramming. Adv Biochem Eng Biotechnol, 2009. 114: p. 185-99. PubMed PMID: 19343304. PubMed Central PMCID...potential of muscle stem cells. Dev Cell, 2001. 1(3): p. 333-42. PubMed PMID: 11702945. 6. Cowan, C.A., et...from human blastocysts. N Engl J Med, 2004. 350(13): p. 1353-6. PubMed PMID: 14999088. 7. Turetsky, T., et...preimplantation genetic diagnosis. Hum Reprod, 2008. 23(1): p. 46-53. PubMed PMID: 17989069. 8. Takahashi, K. and...

...019-1711-4 Chen, P. J., Hussmann, J. A., Yan, J., Knipping, F., Ravisankar, P., Chen, P.-F., Chen, C., ....2021.09.018 Nelson, J. W., Randolph, P. B., Shen, S. P., Everette, K. A., Chen, P. J., Anzalone, A. V., An, M..... V., Randolph, P. B., Davis, J. R., Sousa, A. A., Koblan, L. W., Levy, J. M., Chen, P. J., Wilson, C....j.cell.2014.02.001 Yan, J., Oyler-Castrillo, P., Ravisankar, P., Ward, C. C., Levesque, S., Jing, Y., Simpson...s41587-021-01039-7 Nishimasu, H., Ran, F. A., Hsu, P. D., Konermann, S., Shehata, S. I., Dohmae, N., Ishitani... Emily P. Bentley...

...Cells by Centrifugation. Stem Cells Int, 2016. 2016: p. 1329459. PubMed PMID: 27579041. PubMed Central PMCID...gene expression signatures. Cell Res, 2011. 21(3): p. 518-29. PubMed PMID: 21243013. PubMed Central PMCID...gene therapy for SCID-X1. Science, 2003. 302(5644): p. 415-9. PubMed PMID: 14564000. 5. Seki, T., S. Yuasa...T cells and Sendai virus. Nat Protoc, 2012. 7(4): p. 718-28. PubMed PMID: 22422317. 6. Diecke, S., et ...pluripotent stem cells. Korean J Intern Med, 2014. 29(5): p. 547-57. PubMed PMID: 25228828. 7. Okita, K., et al... peripheral blood cells. Stem Cells, 2013. 31(3): p. 458-66. PubMed PMID: 23193063. 8. Ehrhardt, A., et...vectors for gene therapy. Curr Gene Ther, 2008. 8(3): p. 147-61. PubMed PMID: 18537590. 9. Mack, A.A., et ...

... S L L T C G D V E E N P G P P2A: (GSG) A T N F S L L K Q A G D V E E N P G P E2A: (GSG) Q C T N Y...L K L A G D V E S N P G P F2A: (GSG) V K Q T L N F D L L K L A G D V E S N P G P * (GSG) residues...

...019-1711-4 Chen, P. J., Hussmann, J. A., Yan, J., Knipping, F., Ravisankar, P., Chen, P.-F., Chen, C., ...00868-w Nelson, J. W., Randolph, P. B., Shen, S. P., Everette, K. A., Chen, P. J., Anzalone, A. V., An, M..... V., Randolph, P. B., Davis, J. R., Sousa, A. A., Koblan, L. W., Levy, J. M., Chen, P. J., Wilson, C....s41551-024-01227-1 Yan, J., Oyler-Castrillo, P., Ravisankar, P., Ward, C. C., Levesque, S., Jing, Y., Simpson...Jennifer Tsang in October 2019 and updated by Emily P. Bentley in December 2024. References and Resources...Neugebauer, M. E., An, M., Davis, J. R., Randolph, P. B., McElroy, A., Gao, X. D., Raguram, A., Richter..., L., Vakulskas, C. A., Walker, J. A., Kadina, A. P., Zepeda, A. E., Holden, K., Ma, H., Xie, J., Gao,...

...References and Resources References Gelsinger, D. R., Vo, P. L. H., Klompe, S. E., Ronda, C., Wang, H. H., & Sternberg...doi.org/10.1038/s41596-023-00927-3 Klompe, S. E., Vo, P. L. H., Halpin-Healy, T. S., & Sternberg, S. H. (2019...https://doi.org/10.1073/pnas.1709035114 Witte, I. P., Lampe, G. D., Eitzinger, S., Miller, S. M., Berríos...King, R. T., Stringham, O. G., Gelsinger, D. R., Vo, P. L. H., Chen, A. T., Tolar, J., Osborn, M. J., Sternberg... Emily P. Bentley...

..., E., Serack, F., Harvey, J. W., Brauer, P. P., Pirtle, C. P., Coffey, A., Howard, M., Kamath, N., … &... evolved optimizations for prime editing By Emily P. Bentley The David Liu Lab has made two recent deposits...prime editing installs two sequences containing attB/P recombinase landing sites on opposite DNA strands.... 5’ flaps yields an edited sequence with the attB/P landing site. A recombinase integrates a sequence ..., Banskota, S., Davis, J. R., Newby, G. A., Chen, P. Z., Palczewski, K., & Liu, D. R. (2024). Engineered...   CHARM: A compact epigenetic silencer By Emily P. Bentley The Weissman and Gilbert Labs have previously...

...Haft, D. H., Horvath, P., Moineau, S., Mojica, F. J. M., Terns, R. M., Terns, M. P., White, M. F., Yakunin...Horvath, P., Moineau, S., Mojica, F. J. M., Scott, D., Shah, S. A., Siksnys, V., Terns, M. P., Venclovas...Abudayyeh, O. O., Gootenberg, J. S., Essletzbichler, P., Han, S., Joung, J., Belanto, J. J., Verdine, V.,..., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W., Marraffini, L. A., & Zhang..., Chen, J. S., Paez-Espino, D., Ma, E., Witte, I. P., Cofsky, J. C., Kyrpides, N. C., Banfield, J. F.,...Makarova, K. S., Acuña, L. G., Wolf, Y. I., Covarrubias, P. C., Shmakov, S. A., Silva, C., Tolstoy, I., Johnson...–2012. https://doi.org/10.1093/nar/gkz1197 Samai, P., Pyenson, N., Jiang, W., Goldberg, G. W., Hatoum-...

... Cameron, P., Coons, M. M., Klompe, S. E., Lied, A. M., Smith, S. C., Vidal, B., Donohoue, P. D., Rotstein...Haft, D. H., Horvath, P., Moineau, S., Mojica, F. J. M., Terns, R. M., Terns, M. P., White, M. F., Yakunin...10.1016/j.ggedit.2022.100013 O’Brien, R. E., Bravo, J. P., Ramos, D., Hibshman, G. N., Wright, J. T., & Taylor....024 Morisaka, H., Yoshimi, K., Okuzaki, Y., Gee, P., Kunihiro, Y., Sonpho, E., Xu, H., Sasakawa, N., ...

... of CcdB.   Figure 1: Image from Bernard, P., et al. showing the pKIL 18/19 vectors and demonstrating...guest blog entry of your own! References 1. Bernard, P. “Positive Selection of Recombinant DNA by CcdB.” ...Mar;15(6):1031-7. PubMed PMID: 7623659. 3. Bernard, P., et al. “Positive-selection vectors using the F plasmid...11;148(1):71-4. PubMed PMID: 7926841. 4. Bernard, P., et al. “The F plasmid CcdB protein induces efficient...;234(3):534-41. PubMed PMID: 8254658. 5. Bernard, P. and Couturier, M. “Cell killing by the F plasmid ...

...there is often discussion of “big P” and “little p” policy.  “Big P” policy is what you might typically...scientific areas. Whether you’re interested in “big P” or “little P” policy, the possibilities are endless leading... government itself. You can work to influence Big P policy from a post in non-governmental organizations...formal lobbying, or strategy setting.  The “little p” policy is non-governmental work happening at large...

...., Nalin, R., Sailland, A., Auriol, P., Vogel, T. M., & Simonet, P. (2001). Laboratory-scale evidence ...Jessica Welch in January 2017 and was updated by Emily P. Bentley in October 2024. References and Resources...Veseli, I., Moore, R. M., Kiefl, E., Moody, T., Rice, P. A., Yu, M. K., Mimee, M., Chang, E. B., Ruscheweyh...doi.org/10.1016/j.cell.2024.01.039 Garcillán-Barcia, M. P., Redondo-Salvo, S., & de la Cruz, F. (2023). Plasmid...

...Sands, J., Sahasrabudhe, A., Anikeeva, P., & Chandrakasan, A. P. (2020). Modular Optoelectronic System...EMBC44109.2020.9175600 Park, S., Loke, G., Fink, Y., & Anikeeva, P. (2019). Flexible fiber-based optoelectronics for ...Niroui, F., Fink, Y., Chandrakasan, A., … Anikeeva, P. (2023). Multifunctional microelectronic fibers enable...

...j.cell.2020.04.031 Zimmermann I, Egloff P, Hutter CAJ, Kuhn BT, Bräuer P, Newstead S, Dawson RJP, Geertsma ...s41596-020-0304-x Zimmermann I, Egloff P, Hutter CA, Arnold FM, Stohler P, Bocquet N, Hug MN, Huber S, Siegrist...Siegrist M, Hetemann L, Gera J, Gmür S, Spies P, Gygax D, Geertsma ER, Dawson RJ, Seeger MA (2018) Synthetic...

... protein interactions. J Cell Biol, 2003. 163(2): p. 257-69. PubMed PMID: 14581454. PubMed Central PMCID...microdomains of live cells. Science, 2002. 296(5569): p. 913-6. PubMed PMID: 11988576. 3. Matz, M.V., et al...nonbioluminescent Anthozoa species. Nat Biotechnol, 1999. 17(10): p. 969-73. PubMed PMID: 10504696. 4. Ohashi, T., et ... unstructured proteins. Protein Sci, 2007. 16(7): p. 1429-38. PubMed PMID: 17586775. PubMed Central PMCID...under physiologic conditions. Traffic, 2012. 13(5): p. 643-9. PubMed PMID: 22289035. PubMed Central PMCID...

...s41565-023-01419-x Deverman, B. E., Pravdo, P. L., Simpson, B. P., Kumar, S. R., Chan, K. Y., Banerjee, A...Hordeaux, J., Yuan, Y., Clark, P. M., Wang, Q., Martino, R. A., Sims, J. J., Bell, P., Raymond, A., Stanford,... A. I., Dvoryanchikov, G., Makhoul, V., Tsoulfas, P., & Chaudhari, N. (2022). Selectively Imaging Cranial...Griffiths, J. A., Ichiki, T., Mazmanian, S. K., Osborne, P. B., Keast, J. R., Miller, C. T., Fox, A. S., Chiu... N. M., Sun, J., Chen, X., Lefevre, A., Singh, V. P., Arokiaraj, C. M., Shay, T. F., Vendemiatti, J., ... A., Wu, W.-L., Yang, B., Huber, N., Pasca, S. P., & Gradinaru, V. (2016). Cre-dependent selection yields...Gore, B. B., Szelenyi, E. R., Trader, C., Balaram, P., … Tasic, B. (2021). Enhancer viruses for combinatorial...

....   References Bennardo N, Gunn A, Cheng A, Hasty P, Stark JM (2009) Limiting the Persistence of a Chromosome...10.1371/journal.pgen.1000683 Bétermier M, Bertrand P, Lopez BS (2014) Is Non-Homologous End-Joining Really...Waters CA, Strande NT, Pryor JM, Strom CN, Mieczkowski P, Burkhalter MD, Oh S, Qaqish BF, Moore DT, Hendrickson....1038/ncomms5286 Yan M-Y, Li S-S, Ding X-Y, Guo X-P, Jin Q, Sun Y-C (2020) A CRISPR-Assisted Nonhomologous...

...plasmids and plasmids in incompatibility groups N, P, Q, and W are known to have replicons with broad host...Myxococcus virescens, M. xanthus, Proteus rettgeri, P. mirabilis, Providencia stuartii, Pseudomonas spp....Paracoccous denitrificans, Pseudomonas fluorescens, P. putida, Rhizobium meliloti, R. leguminosarum by. .../10.1074/jbc.273.14.8447  Jain, A., & Srivastava, P. (2013). Broad host range plasmids. FEMS Microbiology...2.50021-0  Toh, W. K., Teo, Y. L., Tor, X. Y., Loh, P. C., & Wong, H. L. (2023). Development of constitutive...

...Kateriya, S., Adeishvili, N., Berthold, P., Ollig, D., Hegemann, P., & Bamberg, E. (2003). Channelrhodopsin...Papagiakoumou, E., Gottschalk, A., Soba, P., Emiliani, V., Engel, A. K., Hegemann, P., & Wiegert, J. S. (2021). BiPOLES...Berndt, A., Sohal, V. S., Deisseroth, K., & Hegemann, P. (2010). Ultrafast optogenetic control. Nature Neuroscience....1936192100 Tichy, A.-M., Gerrard, E. J., Sexton, P. M., & Janovjak, H. (2019). Light-activated chimeric...

...., Feliciano, P. R., Arif, A., Bohnuud, T., Fernandez, T. P., Gehrke, J. M., Grayson, P., Lee, K. D., ...updated by Jennifer Tsang in August 2020 and by Emily P. Bentley in February 2025. References and Resources...Grünewald, J., Zhou, R., Lareau, C. A., Garcia, S. P., Iyer, S., Miller, B. R., Langner, L. M., Hsu, J...., Levy, J. M., Tay, T., Newby, G. A., Maianti, J. P., Raguram, A., & Liu, D. R. (2018). Improving cytidine...McElroy, A. N., Pandey, S., Doman, J. L., Huang, T. P., Raguram, A., Banskota, S., Newby, G. A., Tolar, ...Christie, K. A., Whittaker, M. N., & Kleinstiver, B. P. (2020). Unconstrained genome targeting with near-...

...experiment! References Bikard D, Jiang W, Samai P, Hochschild A, Zhang F, Marraffini LA (2013) Programmable...Chavez A, Scheiman J, Vora S, Pruitt BW, Tuttle M, P R Iyer E, Lin S, Kiani S, Guzman CD, Wiegand DJ, Ter-Ovanesyan... https://doi.org/10.1038/nature14136 Perez-Pinera P, Kocak DD, Vockley CM, Adler AF, Kabadi AM, Polstein...Zhang J, Tang C, Zhang X, Xu H, Shi L, Cheng L, Huang P, Yang H (2018) In vivo simultaneous transcriptional...

...than the substance P peptide, but still quite small. However, unlike the substance P tag, they found that...involved a peptide from the neuropeptide substance P. This protein had a highly specific antibody to the... the tag could be quite small. However, substance P has an amide group added to its C-terminus post-translationally...

...Lecomte E, Tournaire B, Cogné B, Dupont J-B, Lindenbaum P, Martin-Fontaine M, Broucque F, Robin C, Hebben M,...Merten O-W, Blouin V, François A, Redon R, Moullier P, Léger A (2015) Advanced Characterization of DNA Molecules..., Penaud-Budloo M, Broucque F, Blouin V, Moullier P, Ayuso E, Vandenberghe LH (2017) AAV-ID: A Rapid and...

...en.wikipedia.org/wiki/File:Crispr.png Barrangou R, Horvath P. Science. 315, 2007 Jan; 1709–1712 (2007). CRISPRdb..., Barrangou R, Brouns SJJ, Charpentier E, Horvath P, Moineu S, Mojica FJM, Wolf YI, Yakunin JvdO, Koonin... F. Science. 2013 Jan; 339 (6121): 819–23. Mali P, Yang L, Esvelt KM, Aach J, Guell M, DiCarlo JE, Norville...

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... a lentiviral vector. Naldini L, Blömer U, Gallay P, Ory D, Mulligan R, Gage FH, Verma IM, Trono D. Science...Greenberg RN, Kinder C, Zolopa A, Binder-Scholl G, Tebas P, June CH, Humeau LM, Rebello T. J Gene Med. 2013 Feb...Gourmel B, Socie G, Chrétien S, Cartier N, Aubourg P, Fischer A, Cornetta K, Galacteros F, Beuzard Y, Gluckman...Gluckman E, Bushman F, Hacein-Bey-Abina S, Leboulch P. Nature. 2010 Sep 16;467(7313):318-22. PubMed PMID...

...and other important information. Tip: A lowercase "p" is often used as the first letter of a plasmid name...and simply denotes that the object is a ‘plasmid’. “p” is for plasmid. pXXXXX-XXXX Step 1: Backbone name...

...which identifies a p value cut off. This method entails arranging all calculated p values from smallest...this by establishing a significance threshold at a p value less than or equal to the alpha value divided...the cut off for significance by finding the largest p value where its rank divided by the total number of...multiplied by the alpha value is less than or equal to the p value. There are drawbacks to these tests as they ...

... J, Glanville J, Wolf P, Frenzel A, Wong J, Koh XY, Eng H-Y, Lane D, Lefranc M-P, Clark M, Dübel S (2018...doi.org/10.1080/19420862.2018.1445456  Holzlöhner P, Hanack K (2017) Generation of Murine Monoclonal Antibodies...

...Fotouhi, M., Bolivar, S. G., Ruiz Moleon, V., Eckmann, P., Worrall, D., McDowell, I., Southern, K., Reintsch...Bandrowski, A., Virk, H., Edwards, A. M., McPherson, P., & Laflamme, C. (2023). Scaling of an antibody validation...10.12688/f1000research.154034.1 Laflamme, C., McKeever, P. M., Kumar, R., Schwartz, J., Kolahdouzan, M., Chen... A. M., Healy, L. M., Robertson, J., & McPherson, P. S. (2019). Implementation of an antibody characterization...McCarthy, J., Amrani, Y., Duffy, S. M., Bradding, P., & Roach, K. M. (2021). TGFβ1 induces resistance ...Weston, C. A., Chachi, L., Roach, K. M., & Bradding, P. (2019). Validation of antibodies for the specific...

...recognizes a short, linear epitope derived from the P and V proteins of Simian virus 5 (SV5) (Randall et...doi.org/10.57733/addgene.l0ulu4. Hanke, T., Szawlowski, P., & Randall, R. E. (1992). Construction of solid matrix-antibody-antigen...reporting of mechanical forces with PILATeS By Emily P. Bentley Time to get flexible! Alex Dunn’s lab and...plasmids here! Zhong, B. L., Elliot, J. M., Wang, P., Li, H., Hall, R. N., Wang, B., Prakash, M., & Dunn...

...References Abudayyeh OO, Gootenberg JS, Essletzbichler P, Han S, Joung J, Belanto JJ, Verdine V, Cox DBT, Kellner...from this paper at Addgene. Amrani N, Gao XD, Liu P, Edraki A, Mir A, Ibraheim R, Gupta A, Sasaki KE, ...Settle AH, Lied AM, McGovern K, Fuller CK, Cameron P, Fazzio TG, Zhu LJ, Wolfe SA, Sontheimer EJ (2018)...Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin...

...10.1016/B978-0-12-801238-3.02523-X. Hofmann, C., Löser, P., Cichon, G., Arnold, W., Both, G. W., & Strauss, ...doi.org/10.1136/jcp.s3-6.1.39. PMID: 4376152. Rowe, W. P., Huebner, R. J., Gilmore, L. K., Parrott, R. H., ...R. L., Garcia, K. Y., Goodrich, J. S., Lovett, S. P., Parker, K., Radune, D., Hatada, A., Pan, C. Y., ...Rizzo, K., Bertumen, J. B., Morales, C., Oluniyi, P. E., Nguyen, J., … Chiu, C. Y. (2023). Adeno-associated...

...Y, Chik KK-H, Zhang X, Fung AY-F, Tsoi H-W, Cai J-P, Chan W-M, Ip JD, Chu AW-H, Zhou J, Lung DC, Kok K-H...Jia HP, Halabi C, Sigmund CD, Meyerholz DK, Kirby P, Look DC, Perlman S (2006) Lethal Infection of K18...doi.org/10.1128/jvi.02012-06 Ou X, Liu Y, Lei X, Li P, Mi D, Ren L, Guo L, Guo R, Chen T, Hu J, Xiang Z,...

...bloggers Roodolph (Roo) P. St Pierre and Rose C. St Pierre! Roodolph (Roo) P. St Pierre is currently ... was contributed by guest bloggers Roodolph (Roo) P. St Pierre and Rose C. St Pierre, founders of STEAMid...

...N., Flad, K., Schmidt, M. F., Tabor, A., Gmeiner, P., Hübner, H., & Weikert, D. (2020). Fluorescent ligands...78827-9 Breen, C. J., Raverdeau, M., & Voorheis, H. P. (2016). Development of a quantitative fluorescence-based...W. C., Patel, R., Lu, R., Macklin, J. J., Keller, P. J., Ji, N., & Lavis, L. D. (2017). A general method...., Parisi, M., Cincotti, G., Rampioni, G., Visca, P., & Kolmakov, K. (2023). Expanding the microbiologist...Grondin, Y., Rogers, R., Bousfield, D., & Demokritou, P. (2019). Development & characterization of fluorescently...

....org/10.1177/002215549804600813 Erickson, P. A., Lewis, G. P., & Fisher, S. K. (1993). Chapter 15 Postembedding...In J. Sturtevant (Ed.), PLoS ONE (Vol. 7, Issue 6, p. e38313). Public Library of Science (PLoS). https:...

...References: Allocca M, Doria M, Petrillo M, Colella P, Garcia-Hoyos M, Gibbs D, Kim SR, Maguire A, Rex TS...https://doi.org/10.1038/nbt1153 Odom GL, Gregorevic P, Allen JM, Chamberlain JS (2011) Gene Therapy of mdx...://doi.org/10.1038/mt.2010.205 Trapani I, Colella P, Sommella A, Iodice C, Cesi G, Simone S, Marrocco ....201302948 Trapani I, Toriello E, de Simone S, Colella P, Iodice C, Polishchuk EV, Sommella A, Colecchi L, ...