We narrowed to 604 results for: REV

1. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

   Type

   Blog Post

   Published

   June 30, 2020, 1:15 p.m.

   ...That’s another benefit to note-taking: you can revisit the Q&A in the chat box. Presenting? Make it interactive...

2. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

   Type

   Blog Post

   Published

   April 7, 2022, 1:15 p.m.

   ...extrasynaptic glutamate release. Building on the previous generation of the SF-Venus-iGluSnFR (itself an...

3. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

   Type

   Blog Post

   Published

   Sept. 19, 2017, 1:20 p.m.

   ...proteins have been around for awhile (check out this review), but the Bruchez and Tsang labs recently developed...

4. Visualizing Translation at the Single Molecule Level

   Type

   Blog Post

   Published

   Aug. 1, 2017, 1:15 p.m.

   ...the mRNA to the plasma membrane; this sequence prevents diffusion of the mCherry labeled mRNA and keeps...

5. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

   Type

   Blog Post

   Published

   July 21, 2017, 12:48 p.m.

   ...UAS system, and you can read more about them in review articles (2–4). You can also take a look at my ...

6. Predicting Adverse Reactions to Monoclonal Antibody Drugs

   Type

   Blog Post

   Published

   Feb. 13, 2024, 2:15 p.m.

   ...to model individually-specific factors, such as previous exposures to that specific antigen, pre-existing...

7. Viral Vectors 101: Inverted Terminal Repeats

   Type

   Blog Post

   Published

   Oct. 19, 2023, 1:15 p.m.

   ... headache. How do I maintain ITR integrity and prevent unwanted mutations?  During propagation, mutants...

8. Trading Spaces - Visiting Scientist Edition

   Type

   Blog Post

   Published

   Aug. 22, 2023, 1 p.m.

   ... don’t understand something. Offer to help out wherever you can and don’t forget to express your gratitude...

9. Early Career Researcher Toolbox: Free Tools for Science Writing

   Type

   Blog Post

   Published

   April 28, 2021, 1:15 p.m.

   ...reading, and organizing Tips for writing a scientific review article   Write for the Addgene Blog ...

10. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

    Type

    Blog Post

    Published

    Dec. 10, 2019, 1:58 p.m.

    ... muscle cells. These changes were larger than previously reported for other organisms, and the Gottschalk...

11. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type

    Blog Post

    Published

    Nov. 27, 2018, 2 p.m.

    ... in 2016 and 2017 Listen to Our Interview with Previous Awardee Kwabena Duedu Resources at Addgene.org...

12. Four Factors that Differentiate the Stem Cell Field

    Type

    Blog Post

    Published

    Sept. 28, 2016, 2:35 p.m.

    ...regarding topics such as informed consent (How do we prevent stem cell lines from becoming the new HeLa cells...

13. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type

    Blog Post

    Published

    March 24, 2016, 2:30 p.m.

    ...question. Our recent paper (Richardson et al 2016) revealed some of the biophysical parameters that can influence...

14. Resume Writing for Non-academic Science Careers

    Type

    Blog Post

    Published

    Dec. 18, 2014, 4:06 p.m.

    ...With a resume however, most often people choose a reversed chronological style (the latest to the earliest...

15. Finding and Joining Your Dream Lab

    Type

    Blog Post

    Published

    April 8, 2014, 2:50 p.m.

    ...students typically go through a rotation, which reveals their working habits, personality, and gives them...

16. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

    Type

    Blog Post

    Published

    Feb. 19, 2019, 1:36 p.m.

    ... University School of Law, where she was on law review. She clerked for Juan R. Torruella, U.S. First ...

17. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type

    Blog Post

    Published

    Sept. 15, 2020, 1:15 p.m.

    ...survive (ex: antibiotic resistance marker). In a previous blog post, we covered how to use positive and ...

18. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type

    Blog Post

    Published

    Sept. 26, 2024, 1:15 p.m.

    ...or polymerization will occur at that position, preventing T4 from chewing back the entire 10–12 bases. ...

19. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type

    Blog Post

    Published

    Aug. 20, 2024, 1:15 p.m.

    ...different from the yes-no histogram plot shown previously. Here, the orange peak is a baseline expression...

20. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type

    Blog Post

    Published

    Aug. 6, 2024, 1:15 p.m.

    ...components. Pol Precursor protein containing Reverse Transcriptase and Integrase components. Transfer...

21. Hot Plasmids - May 2022

    Type

    Blog Post

    Published

    May 26, 2022, 1:15 p.m.

    ... Brian O'Neill  The Viviana Gradinaru Lab has previously developed the multiplexed version of the Cre-...

22. AAV Q&A with Tim Miles

    Type

    Blog Post

    Published

    May 17, 2022, 1:15 p.m.

    ...epigenetic editing. This area is discussed in our recent review.      And that's a wrap on our AAV webinar! Special...

23. Building and Validating Recombinant Antibodies: A Case Study

    Type

    Blog Post

    Published

    April 5, 2022, 1:20 p.m.

    ...visualized in a single experiment, which had not been previously possible. This advancement could be especially...

24. Antibodies 101: Buffers, Storage, and Conjugates

    Type

    Blog Post

    Published

    Feb. 1, 2022, 2:15 p.m.

    ...dark, opaque vial or wrapped in aluminum foil to prevent ambient light from degrading them. You’ll also ...

25. Antibodies 101: Isotypes

    Type

    Blog Post

    Published

    Oct. 19, 2021, 1:15 p.m.

    ... common antibody after IgG; but it's the most prevalent antibody in secretions (e.g. tears, saliva, mucus...

26. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type

    Blog Post

    Published

    July 28, 2017, 1:30 p.m.

    ...immunology PhD student at Harvard University. In my previous two posts, I’ve described the fundamentals of ...

27. Improved Plasmid Maps Powered by SnapGene

    Type

    Blog Post

    Published

    June 29, 2017, 1:06 p.m.

    ...enzymes that can be detected is 5x greater than our previous mapping software and now includes Type IIS restriction...

28. Sharing is Caring: But How to Distribute Open Hardware?

    Type

    Blog Post

    Published

    Feb. 28, 2023, 2:15 p.m.

    ...computational processing with biological protocols and revolutionary physical approaches. However, with each new ...

29. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

    Type

    Blog Post

    Published

    March 17, 2020, 1:32 p.m.

    ... more precise base editors capable of editing previously uneditable sequences. Four DNA base swaps, so...

30. Screening for Successful Genome Editing with Digital PCR

    Type

    Blog Post

    Published

    March 30, 2017, 2:30 p.m.

    ...detect NHEJ. These assays include a forward and reverse primer to amplify the target locus, as well as ...

31. Top Requested Lentivirus and AAV of 2016

    Type

    Blog Post

    Published

    Jan. 6, 2017, 3:56 p.m.

    ..., Maratos-Flier E, Roth BL, Lowell BB. Rapid, reversible activation of AgRP neurons drives feeding behavior...

32. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type

    Blog Post

    Published

    June 2, 2016, 2:30 p.m.

    ...Careful documentation will keep you on task and will prevent you from getting lost in the wide word of informatics...

33. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type

    Blog Post

    Published

    Feb. 10, 2016, 3:30 p.m.

    ...familiar territory, but quickly diverges from previously discussed methods. As described above, you design...

34. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type

    Blog Post

    Published

    July 10, 2014, 7:17 p.m.

    ... Assembly Standards Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)    ...

35. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type

    Blog Post

    Published

    Dec. 10, 2013, 3:13 p.m.

    ...chance they will bind that sequence. That’s the revolutionary aspect: TALENs take a technology that was difficult...

36. Seven Tips for Using LinkedIn as a Scientist

    Type

    Blog Post

    Published

    Oct. 1, 2019, 1:21 p.m.

    ...Person.” If that happens a few times, LinkedIn may revoke your ability to send invitations. Finally, if you...

37. Troubleshooting Your Plasmid Cloning Experiment

    Type

    Blog Post

    Published

    Sept. 24, 2019, 12:55 p.m.

    ...fragments and you will remove the buffers used in the previous reactions. This will increase your chances to ...

38. dTAG - You're it!

    Type

    Blog Post

    Published

    June 21, 2018, 2:06 p.m.

    ...the proteasome to destroy targeted proteins. We previously developed small molecule degraders that achieve...

39. RANbodies: Reporter Nanobody Fusions

    Type

    Blog Post

    Published

    April 10, 2018, 12:56 p.m.

    ...To create their RANbodies, Yamagata et al used previously published nanobody sequences that targeted the...

40. Giving gRNAs a Facelift - Synthetic and Beyond

    Type

    Blog Post

    Published

    Oct. 20, 2022, 1:15 p.m.

    ...increase stability.  Nature has evolved ways to prevent degradation of important cellular RNA molecules...

41. Antibodies 101: Selecting the Right Antibody

    Type

    Blog Post

    Published

    March 15, 2022, 1:15 p.m.

    ...can narrow them down in a variety of ways. First, review the citations. An antibody that has been cited ...

42. Antibodies 101: Secondary Antibodies

    Type

    Blog Post

    Published

    Dec. 21, 2021, 2:15 p.m.

    ...the antibody binds to a protein, and then voila! Wherever your protein of interest is, a signal will also...

43. Transferable Skills Guide: Problem Solving

    Type

    Blog Post

    Published

    Nov. 30, 2021, 2:15 p.m.

    ...strategy was. If you didn’t quite fix everything or revealed another issue in the process, you might need to...

44. What's New in CRISPR - November 2021

    Type

    Blog Post

    Published

    Nov. 18, 2021, 2:15 p.m.

    ... PE-Cas9 comprises an active Cas9 fused to the reverse transcriptase (RT), which induces two double-strand...

45. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type

    Blog Post

    Published

    Aug. 5, 2021, 12:15 p.m.

    ...(PV)-expressing neurons, neurons that the lab previously found had a role in perceiving touch inputs. ...

46. Human Germline Editing Using CRISPR

    Type

    Blog Post

    Published

    Aug. 10, 2017, 2:19 p.m.

    ...germline editing is permissible in cases of disease prevention. However, it is important to consider that already-tested...

47. The time and cost required to make a plasmid

    Type

    Blog Post

    Published

    March 23, 2023, 1:15 p.m.

    ..., you’ll be able to use or build on work done previously, borrow small aliquots of restriction enzymes...

48. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type

    Blog Post

    Published

    Dec. 8, 2022, 2:15 p.m.

    ...their own unique features and quirks. Here, we will review the genome engineering abilities of the lesser-...

49. Visualizing Protein Turnover In Situ

    Type

    Blog Post

    Published

    Jan. 16, 2018, 3:20 p.m.

    ...Singer. "Single β-actin mRNA detection in neurons reveals a mechanism for regulating its translatability....

50. Antibodies 101: Polyclonal Antibodies

    Type

    Blog Post

    Published

    July 14, 2021, 1:15 p.m.

    ...stay away from polyclonal antibodies The most prevalent issue with polyclonal antibodies is their batch...