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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 414 results for: vent

Showing: 181 - 200 of 414 results

  1. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ...collaboration and education in protein science, IPI hosts events and training courses on technologies and laboratory... on the IPI website and stay informed on future events by signing up for the IPI newsletter. The collection...

  2. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ...expressed for a short period of time, which would prevent the accumulation of unwanted mutations over time...upregulating HDR, NHEJ must be downregulated to prevent the introduction of new mutations by CRISPR; editing...

  3. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...The blood-brain-barrier (BBB) exists to prevent substances in the blood, like proteins and viruses, from...ultrasound (FUS), intracorporeal (IC), intracerebroventricular (ICV), intramuscular (IM), intrathecal ...interest, while miRNA target sites can be used to prevent expression in cell types not of interest. Systemic...

  4. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    Published
    Oct. 2, 2018, 12:56 p.m.
    ...intimidation, sexual harassment Isolation – including preventing access to opportunities, physical or social isolation...develop transferable skills by attending career events or being involved in a grad or postdoc association...

  5. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...the LOV domain blocks the enzyme binding domain preventing downstream signaling pathways. Light exposure...optimize the intensities of light sources used to prevent activation of both tools with each light source...

  6. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...glutamate molecules are released in each synaptic event and they are rapidly taken up by postsynaptic receptors...iGluSnFR4f is well-suited for imaging rapid synaptic events such as in the somatosensory cortex, while iGluSnFR4s...

  7. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...for genome sequencing or annotation (5). In the event that genome sequencing can be done, this technique...functional cell-binding proteins in the (all-too-common) event that homology-based searches return no candidates...

  9. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ...assembly-regulating proteins in vitro reveals complex events. a) Micrographs of a fluorescently-labeled actin...Intensity and filament length traces for a different event where CP appears briefly then leaves. Images reused...

  12. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...molecules that would otherwise be missed with conventional FP imaging. PA-FPs are divided into 2 main categories...although PA-FPs are vastly improved over the conventional FPs for super-resolution imaging, they still...

  13. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...time. One system you could use is pX333 from the Ventura lab. pX333, a modification of pX330, contains humanized...and thus the highest frequency of genome editing events. Yamamoto Lab Multiplex CRISPR/Cas9 Assembly Kit...

  14. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ...prime editing efficiency can vary widely — so its inventors kept optimizing it. Using a pooled CRISPRi screen...either selectively excise the edited DNA flap or prevent it from hybridizing with the unedited strand. Temporarily...

  15. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ... of Cas9, the fluorescent domains dimerize and prevent DNA binding in the dark. Upon light stimulation...genome editing, reduce off-target activity, and prevent cytotoxic effects. For a more detailed review, ...

  16. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...inhibitor of uracil DNA glycosylase (UGI) to dCas9 prevents base excision repair, which would change the U...of inosine from DNA is likely infrequent, thus preventing the induction of base excision repair. In terms...

  17. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...transfer buffers. To slow down smaller proteins and prevent them from over-transferring, increase the amount... away the SDS. To speed up larger proteins and prevent them from under-transferring, try increasing the...

  18. Lucky Thirteen: Guest Contributors of 2023

    Type
    Blog Post
    Published
    Dec. 5, 2023, 2:15 p.m.
    ...Synthetic BiologyAuthor: Niko McCartyAntibodies 101: Conventional vs. Flow Spectrometry Author: Lila WittTips ...

  19. Our New Antibody Mascot is....

    Type
    Blog Post
    Published
    Feb. 24, 2022, 2:15 p.m.
    ...mascot, an antibody joining us on our scientific adventures.  You can submit your name suggestions here or...
Showing: 181 - 200 of 414 results