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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 604 results for: REV

Showing: 501 - 550 of 604 results

  1. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ...stay away from polyclonal antibodies The most prevalent issue with polyclonal antibodies is their batch...

  2. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...or TE buffer, store them in a -80°C freezer to prevent RNA degradation. They will degrade in a -20°C freezer...

  5. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    Published
    Aug. 27, 2015, 2:30 p.m.
    ...formation of the desired ligation product is irreversible because this construct does not retain the enzyme...

  7. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ...regulatory molecules, and has provided clues to their previously uncharacterized cellular roles. In the current...

  10. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ...benefiting from the fruits of scientific research, and prevent technological advances from exacerbating existing...

  13. Lab Automation at Addgene

    Type
    Blog Post
    Published
    March 22, 2018, 1:28 p.m.
    ...decided on Beckman Coulter’s Biomek FXP. We had a previous relationship with Beckman Coulter and that certainly...

  15. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ...adult-born granule cells over time that were not previously possible.   Bensussen S, et al., iScience 2020...

  20. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ...surprised by how multi-purpose it can be. Let’s review a list of MoClo applications. Genetic circuits ...

  21. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ...and PAM-flexible nucleases! In this blog, we will review Cas enzymes, which have a flexible or non-existent...

  22. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...(NHP) brains.  Systemic gene delivery capsids previously identified in rodents have not been widely successful...

  23. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ...nucleus-targeted GCaMP6 with an extra apoCaM-binding motif to prevent aberrant binding of the biosensor to endogenous...

  24. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    .../364/6436/eaav5282.long Redfern CH, Coward P, Degtyarev MY, Lee EK, Kwa AT, Hennighausen L, Bujard H, ...

  27. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...editing with a single Cas9-VPR in Saccharomyces cerevisiae. To control Cas9 activity and influence DNA repair...

  28. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ... libraries were originally described in a peer-reviewed scientific publication. We love science, but we...

  37. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...new knockout animal models in species that were previously difficult to engineer, like rats. If you have...

  42. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...authorial contributions to, and support for, this and previous Addgene CRISPR publications.  References and ...

  43. Common Injection Routes in Mice

    Type
    Blog Post
    Published
    Jan. 22, 2025, 9:42 p.m.
    ...through a feeding tube with a bulb on the end, which prevents accidental tears or punctures to the throat. The...

  44. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...Munro S, Pelham HRB (1987) A C-terminal signal prevents secretion of luminal ER proteins. Cell 48:899–...

  45. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...; at least you know you have an answer to that previous nosy question! The Application Process Broadly...

  46. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...The only difference is that your paper will be reviewed by an internal committee before submission to ...

  49. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ...special? A major advantage of CRISPR/Cas9 over previous genome editing techniques is its simplicity and...

  50. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...ITAMs ensures effective negative selection and prevents autoimmunity. Holst et al (Nat Immunol. 2008 Jun...
Showing: 501 - 550 of 604 results