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We narrowed to 809 results for: nin

Showing: 501 - 550 of 809 results

  1. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...Addgene transfer plasmids are replication-deficient meaning they can be used to create virus that is capable...HIV-derived. Get Tips on Titering Your Lentivirus Concerning safety of lentiviral transduction Q8: You mentioned...our eBook: Plasmids 101 Learn about genome-wide screening with CRISPR and Lentivirus Read about the ClonTracr...

  2. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type
    Blog Post
    Published
    April 2, 2019, 1:44 p.m.
    ...determination, and anterior-posterior segmentation (Jennings and Ish-Horowicz, 2008). Fruit flies that contain...when she pricked her finger on the spindle of a spinning wheel but also a biological transposon system....: 21314430. PubMed Central PMCID: PMC3130349. Jennings, Barbara H., and David Ish-Horowicz. "The Groucho...

  3. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...antibodies. The most famous ones are influenza hemagglutinin (HA), myelocytomatosis viral oncogene (myc),...Looger’s lab have developed new molecular tags combining the advantages of both (Viswanathan, 2015). For... in better resolution images. Conclusion By combining advantages of FPs and epitope tags, smFPs appear...

  4. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ...eventually called aptamers, from the Latin aptus, meaning “to fit”. SELEX could be used to classify DNA aptamers... and tips from experts on FRET techniques. By combining standard donor/acceptor FRET know-how with aptamers...Chemistry 27(2):108-117 · February 2008. 11. Svensen, Nina and Jaffrey, Samie R. “Fluorescent RNA Aptamers ...

  5. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...neuronal tracing or peripheral gene delivery. Combining the rabies glycoprotein into the lentiviral envelope...Table 1 (Gutierrez-Guerrero et al., 2020) Table 1 (Cronin et al., 2005)     References and Resources References...Viruses 12:513 . https://doi.org/10.3390/v12050513 Cronin J, Zhang X-Y, Reiser J (2005) Altering the Tropism...

  6. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ...this point, subsequent introductions of pTargetF containing another sgRNA targeting another site of interest...specific sequences on pTargetF with the ccdB gene containing BsaI restriction enzyme sites at its ends to ...chromosomal integration of cargo without using screening markers. Capability to construct a strain library...

  7. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    Published
    May 20, 2025, 1:15 p.m.
    ...transient transfection, the vector remains episomal, meaning it stays separate from the genome. One option for...replicate during the course of your experiment, meaning you don’t have to worry about vector replication...example, if you are using E. coli simply for easy cloning and quick DNA production, you probably don’t need...

  8. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ...their lab, in their experiments, to enable virtual learning during the pandemic. Feedback like that lets us... easier access to export-controlled items by streamlining permissions and paperwork. Through updating ...across the globe?” By asking this question, and by listening to the answers from our community, we’ll be able...

  9. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...Construction of solid matrix-antibody-antigen complexes containing simian immunodeficiency virus p27 using tag-specific... revealing antigenic differences between human, canine and simian isolates. J Gen Virol., 68 (Pt 11), ...transformed with Cas9 on an integrative plasmid containing tetracycline repressor TetR and resistance selection...

  10. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ... Flow data is messy, so learning how to gate takes a lot longer than learning how to read a gating strategy...often easier to identify different populations by imagining (or drawing) a quadrant over the dot plot. Created...

  11. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...to the AMA, we had discussed the possibility of joining the conversation on Reddit, but it wasn’t until... pleased to see graduate and postdoc programs beginning to support diverse career path development--but...resources. The internet content for scientists in training is abundant, helpful, and sometimes excellent....

  12. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...activation experiments often require quite a bit of tuning before you know your system is working as expected...the best uses for Cas9 activators is in genetic screening. gRNAs targeting every gene in the human genome...Villalta JE, Chen Y, Whitehead EH, Guimaraes C, Panning B, Ploegh HL, Bassik MC, Qi LS, Kampmann M, Weissman...

  13. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ...highly technical and this has a natural, albeit unintentional, tendency to work against transparency. This...will be in danger of having to deliver on well-meaning but impractical requirements imposed by publishers...Toronto, Canada where he studies protein-serine/threonine kinases implicated in human diseases. His spare...

  14. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...factors, therefore promoters play a large role in determining where and when your gene of interest will be ... UAS General expression mRNA Yeast promoter containing Gal4 binding sites, commonly used in Drosophila...increases expression of the repressor 10x, thus tightening regulation of lac promoter. Good for modulating...

  15. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...world manifesting as a full-fledged pandemic. Containing the spread of this virus has become an utmost...earlier epicenters of the outbreak ultimately flattening the curve, it has become evident that widespread...beads. Fluorescently labelled dCas9 complexes containing sgRNA that have no mismatches bind to the immobilised...

  16. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    Published
    April 8, 2025, 1:15 p.m.
    ... E., & Gomes, A. V., “Stain-Free total protein staining is a superior loading control to β-actin for Western...more accurate as a loading control. Thanks for joining us in this exploration of western blot normalization... Gomes, A. V. (2013). Stain-Free total protein staining is a superior loading control to β-actin for Western...

  17. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    Published
    May 18, 2018, 7:32 p.m.
    ...(1:24 - 29:31), there are a variety of ways of defining scientific reproducibility. For a study to be ...even simple procedures like pouring a plate or running a gel have intricacies that aren’t always mentioned...the biological sciences. I’d highly recommend listening to the panel discussion to learn a bit more about...

  18. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ...”. One of my professors was excellent at phone cloning with labs around the world and had specific strategies...absorption by tissues. To avoid absorbance of heme-containing proteins in whole animals, light at or above ... is composed of Venus fluorescent protein, the Troponin C Ca++ binding domain, and NanoLuc® Luciferase...

  19. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...two vectors are part of the larger pMVP/pMAGIC cloning system, which contains Cas9 fusions for several...Arlotto ME, et al (2018) Creation of versatile cloning platforms for transgene expression and dCas9-based...CRISPR–Cas9 epigenome editing enables high-throughput screening for functional regulatory elements in the human...

  20. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 3:42 p.m.
    ...integrins3 Hepatocyte growth factor receptor (HGFR)3 Laminin receptor (LR)3 CD93 AAV5 2,3-N-linked sialic...growth factor receptor (PDGFR) α and β3 AAV8 Laminin receptor (LR)3 AAV receptor2   AAV9 Terminal...Terminal N-linked galactose3 AAV receptor2 Laminin receptor (LR)3 Putative integrin3 1Wu, Miller...

  21. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ...2], [3]. Simply put, the piggyBac™ transposon containing a selection marker is included in a standard ...template to facilitate the selection of cells containing your desired edit. The selection is marker is...transposase enzyme [4]. By using a donor plasmid containing a negative selection marker such as thymidine...

  22. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    Published
    May 27, 2025, 1:15 p.m.
    ...degraded. Cloning Grade DNA (high quality/low quantity) – You can usually complete a cloning experiment...matter of preference.   Which size kit to use?  Obtaining higher quantities of DNA generally means starting...

  23. Congrats to the 2019 Deck The Lab Winners

    Type
    Blog Post
    Published
    Jan. 17, 2020, 3 p.m.
    ...the second year in a row, agar art is one of the winning decorations for Deck The Lab. These festive, living...Kingdom. Kravitz Lab 2019 Holiday Door is up and running! @LexKravitz pic.twitter.com/J5bjHQo565 — Jess ...

  24. The Twelve Days of CRISPR

    Type
    Blog Post
    Published
    Dec. 22, 2020, 2:15 p.m.
    ...CRISPR, Addgene gave to me: tips and tricks for designing gRNAs for CRISPR editing. On the fourth day of...Addgene gave to me: anti-CRISPR proteins!  On the ninth day of CRISPR, Addgene gave to me: RNA editing with...

  25. New Videos: Addgene Lab Tips

    Type
    Blog Post
    Published
    Feb. 15, 2018, 2:41 p.m.
    ... to basic lab etiquette that will keep the lab running like a well-oiled machine. Lab tip #1: Glove trick...and press! Lab tip #2: Tube flick trick When combining reagents in the lab, it is important to mix them...

  26. Expanded Access to Rabies Plasmids

    Type
    Blog Post
    Published
    Feb. 1, 2024, 2:15 p.m.
    ... us to ship rabies plasmids and viral vectors containing genetic elements from rabies plasmids to international...allows Addgene to ship plasmids or viral vectors containing sequences from rabies and all other lyssa genus...

  27. Celebrating the 2024 Nobel Prize in Chemistry

    Type
    Blog Post
    Published
    Oct. 17, 2024, 1:15 p.m.
    ...Hassabis and John Jumper! The Baker Lab focuses on designing entirely new proteins, improving our understanding...least that we’ve seen so far!). They started by designing a novel topology, then iteratively generated 3D...

  28. 8 Great Depositor Research Stories

    Type
    Blog Post
    Published
    Dec. 15, 2022, 2:15 p.m.
    ...blogger Ava Shipman explains how the Wu lab is screening over 3,000 antibodies for use in mice and human...really incredible science - I hope you've enjoyed learning about it (and maybe even picked up a new technique...

  29. Introducing Addgene's Microbiology Pages

    Type
    Blog Post
    Published
    June 13, 2016, 7:08 p.m.
    ... fluorescent proteins, synthetic biology, gene cloning and expression, as well as guide pages for Synthetic...more help with CRISPR, read our recent blog post explaining how CRISPR is being used for bacterial genome...

  30. Call for Guest Bloggers

    Type
    Blog Post
    Published
    March 2, 2016, 3 p.m.
    ...your blog on a variety of social media outlets meaning you’ll have the opportunity to interact with and...different people. Recent Guest Writers Have Included: Wenning Qin, Molecular biologist with Biogen interested...

  31. Countdown to Halloween @Addgene

    Type
    Blog Post
    Published
    Oct. 29, 2014, 5:26 p.m.
    ... costumes). All of this costume planning is for a good cause… Winning! The team with the best costume ...

  32. Congratulations, Deck The Lab winners!

    Type
    Blog Post
    Published
    Jan. 14, 2021, 1:30 p.m.
    ...druryhannah) The Tarling-Vallim Lab’s first place-winning entry doubled as their lab’s decoration competition..., 2020   Deck the Lab: Home Edition Winners Winning first place in the home edition, the MicrobiGals...

  35. MassBioEd Career Exploration Day at Addgene

    Type
    Blog Post
    Published
    Nov. 13, 2015, 3:30 p.m.
    ...that it’s never too early to start networking! Learning about careers at Addgene Afterwards, the students...career paths, Michelle Healy said “You are always learning and that means it is possible to switch your field...

  36. More Data for You: Find Articles Citing Addgene Plasmids

    Type
    Blog Post
    Published
    June 16, 2015, 2:15 p.m.
    ... help save you from performing any unnecessary cloning - the list of "Associated Plasmids" from these ...associated with this "Citing Article" - such as cloning their gene of interest into a previously deposited...

  39. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    Published
    Jan. 28, 2014, 3:10 p.m.
    ...competent cells and using a little-known reagent for streamlining the transformation step. Read on to find protocols...purchasing competent cells. However, for most everyday cloning, homemade competent cells will work just fine.  ...

  42. Editor's Choice, August 2016

    Type
    Blog Post
    Published
    Sept. 2, 2016, 1:32 p.m.
    ...having trouble getting NgAgo genome editing up and running in their labs. Pooran surveyed a CRISPR google ... need to get back to emailing (hopefully non-vacationing) scientists. You can find all of our August posts...

  43. From our Table to Yours: An Inside Look at Lunch at Addgene

    Type
    Blog Post
    Published
    Nov. 27, 2013, 2:54 p.m.
    ...future, and hopefully include some of our favorite winning recipes. Hack Day lunches These days everyone ...project. These projects could be anything from cleaning out the freezers to writing new plasmid protocols...

  45. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ...labs that already have created a transgenic GFP-containing organism,” says Hanson, “using a gene encoding...microscopy enthusiasts! Addgene has empty backbones containing GFP variants used by Sattarzadeh et al., as well...

  46. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

    Type
    Blog Post
    Published
    Dec. 5, 2019, 1:49 p.m.
    ...check it out!). After months of strategizing, designing, developing, testing - and repeat - we’re proud... a make-over as well. While in the course of redesigning the homepage, and striving to tell our story ...

  47. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    Published
    Sept. 29, 2015, 2:30 p.m.
    ...large genes, smaller binary vectors with a larger cloning capacity would be very useful: enter pSIM24! To...customized due to its modular structure. Two multiple cloning sites (MCS's) flank the M24 promoter, allowing ...

  48. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    Published
    April 1, 2014, 5:33 p.m.
    ...Tom Ellis, and George Church for answering our burning questions about how they manage and motivate their...Advice for Choosing a Research Project Finding and Joining Your Dream Lab Advice for Choosing a Research Project...

  50. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    Published
    Feb. 13, 2018, 2:51 p.m.
    ...whole animal models like Drosophila melanogaster, obtaining enough material for a ChIP-seq experiment can ..., the Henikoff lab published a preprint paper outlining improvements to the initial CUT&RUN protocol, ...
Showing: 501 - 550 of 809 results