We narrowed to 293 results for: gne

1. Viral Vectors 101: Virus Safety

   Type

   Blog Post

   Published

   June 22, 2023, 1:15 p.m.

   ...that are built into how something functions, were designed for this purpose. One such control is dividing...

2. Antibodies 101: Choosing the Right Isotype

   Type

   Blog Post

   Published

   April 6, 2023, 3 p.m.

   ...your application, of course!) When the stars are aligned and the lab gods are smiling down at you, you may...

3. Hot Plasmids and Viral Preps - March 2021

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   Blog Post

   Published

   March 23, 2021, 1:15 p.m.

   ...for Borrelia burgdorferi from Christine Jacobs-Wagner’s lab produce different levels of dCas9 expression...

4. Synthetic promoter AAVs for cell-type specific expression in retinal cells

   Type

   Blog Post

   Published

   March 12, 2020, 1:15 p.m.

   ...promoters with the help of epigenetic analysis and designed using stretches of DNA that were transcriptionally...

5. Adapting Toehold Switches to Detect Zika Virus

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   Blog Post

   Published

   Aug. 30, 2016, 2:30 p.m.

   ...Alexander A., et al. "Toehold switches: de-novo-designed regulators of gene expression." Cell 159.4 (2014...

6. 5 Tips for Troubleshooting Viral Transductions

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   Blog Post

   Published

   Aug. 11, 2016, 2:23 p.m.

   ... try amplifying the viral vector with bacteria designed to minimize rearrangements such as NEB Stable ...

7. Cpf1 Update: Comparison to Cas9 and NgAgo

   Type

   Blog Post

   Published

   July 14, 2016, 2:30 p.m.

   ...to examine Cpf1 off-target editing. First, they designed gRNAs with single and double mismatches throughout...

8. Plasmids 101: FLEx Vectors

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   Blog Post

   Published

   April 28, 2016, 2:30 p.m.

   ...switches? FLEx (or “flip-excision”) switches were designed as a genetic tool for researchers to conditionally...

9. Plasmids 101: Terminators and PolyA signals

   Type

   Blog Post

   Published

   March 31, 2016, 2:30 p.m.

   ...Plasmids designed to express genes in a given host cell type are generally broken down into two broad...

10. Tips for Titering Your Lentiviral Preps

    Type

    Blog Post

    Published

    March 15, 2016, 2:30 p.m.

    ...virus will not only ensure that your infection is designed in the best possible way, but it may also save...

11. Gaining Leadership Skills Volunteering at a Professional Organization

    Type

    Blog Post

    Published

    Aug. 6, 2015, 2:30 p.m.

    ... the overall voice may not always be perfectly aligned with my personal viewpoint but, as a leader in ...

12. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type

    Blog Post

    Published

    Jan. 21, 2015, 1:30 p.m.

    ...resolved. If there are any forms that need to be signed, or manuscripts that need to be reviewed, bring...

13. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type

    Blog Post

    Published

    Feb. 7, 2019, 2:16 p.m.

    ...part. For example, you can analyze experiments designed outside CrispyCrunch, or you can input pre-existing...

14. Plasmids 101: 5 factors to help you choose the right cloning method

    Type

    Blog Post

    Published

    Aug. 21, 2018, 12:31 p.m.

    ...amazing project. You’ve written your protocols, designed your experiments, and prepared your reagents. ...

15. Plasmids 101: Protein Expression

    Type

    Blog Post

    Published

    June 7, 2018, 1:17 p.m.

    ...for proper protein function. Most DNA constructs designed for mammalian expression utilize viral promoters...

16. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type

    Blog Post

    Published

    March 28, 2018, 6:52 p.m.

    ...bacterial polymerase subunit ω. This construct is designed to activate transcription from the accessory plasmid...

17. 3D Printing Meets CRISPR Cas9

    Type

    Blog Post

    Published

    Oct. 17, 2017, 12:55 p.m.

    ...check out our hands-on teaching tools that are designed to make the molecular world real for students....

18. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type

    Blog Post

    Published

    Oct. 12, 2017, 2:26 p.m.

    ... not work with C. elegans, several groups have designed modular toolkits to assist with tagging genomic...

19. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type

    Blog Post

    Published

    Dec. 10, 2020, 1 p.m.

    ...targeted organs or tissues with Cas9 and a gRNA designed to target a mutated gene. In a Scientific Reports...

20. Hot Plasmids: Summer 2025

    Type

    Blog Post

    Published

    Aug. 28, 2025, 1:15 p.m.

    ... Huang, H., et al. (2025). ATLAS: a rationally designed anterograde transsynaptic tracer. Nature Methods...