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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 223 results for: his-tag

Showing: 201 - 220 of 223 results

  1. Make a Splash: Notions of Scientific Impact Are Evolving

    Type
    Blog Post
    Published
    Dec. 5, 2014, 2:54 p.m.
    ...articles, Anurag Acharya and colleagues report, the percentage of citations to articles in non-elite journals... recently reported that the most cited work in history is a 1951 description of an assay to determine ...

  2. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    Published
    June 26, 2017, 1:31 p.m.
    ...modified from Ke et al. 2017. 1. Among scientists, historians have the most Twitter users. Biologists rank .... The #1 (re)tweeted domain by scientists was Instagram Social media and news sites were also popular....

  3. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    Published
    Jan. 30, 2014, 3:29 p.m.
    ...Additionally, the presence of a plasmid is disadvantageous from the bacterium's perspective – a plasmid-containing...How else can antibiotics be used in the lab? Historically, antibiotics have also been used to disrupt ...

  4. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ...CRISPR tools, without compromising their strategic advantages in gene editing, while keeping the system to ...multiplex genome engineering of crops or allow for sophisticated CRISPR screenings that would perturb the unprecedented...

  5. Science Career Options

    Type
    Blog Post
    Published
    Feb. 23, 2017, 1:30 p.m.
    ...technology, and good computer skills will give you an advantage. Strong writing, computer skills (Word, Excel,...hobbies and volunteer experiences, as well as work history can really show that you have some of the abilities...

  6. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ...sticky ethical dilemmas to dark episodes in the history of eugenics and research misconduct. Past ethical...who are interested in such careers should take advantage of their academic environment and attend seminars...

  7. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    ...have enhanced the ability of labs to answer sophisticated questions about cellular processes. Currently... et al., 2008), and monitor neuronal activity (voltage sensors), to name a few.   Find Many FRET-Based...

  8. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ... PAM requirement, and how these proteins are advantageous in today’s genome editing landscape. The need...Engineering a CRISPR Variant with PAM Flexibility History of CRISPR Cas – A Tale of Survival and Evolution...

  9. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ...isotypes and subclasses. They each have their own advantages. Since monoclonal antibodies contain a single...Antibody to Mouse Tissue Sections. In Journal of Histochemistry & Cytochemistry (Vol. 46, Issue 8, pp. ...

  10. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ...they should not be relied on exclusively because historical studies of pathogen research have conclusively...628 (2014). PubMed. Gantz, V. M. & Bier, E. The mutagenic chain reaction: A method for converting heterozygous...

  11. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ... 10 more years to obtain FDA approval and make history in the form of Luxturna™. A better vector for ...Naldini,L., 2015. Gene Therapy returns to centre stage. Nature Vol 526, p.351. PubMed PMID: 26469046. 6...

  12. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...expansion.  In some cases, labels are added after this stage.  Step 4: Expand! (Typically by just adding water... L (2022) Super-resolution microscopy: a brief history and new avenues. Philosophical Transactions Royal...

  13. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ...post-injection. Livers from these mice were histologically normal and liver injury markers were not increased...also be packaged into AAV. NmeCas9 has the added advantage that it can be turned off by anti-CRISPR proteins...

  14. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...as well as health-related single nucleotide polymorphisms (SNPs) from human saliva. SHERLOCKv2 provides... a different PAM site and generates 5’ and 3’ staggered ends after dsDNA breaks. The Doudna lab discovered...

  15. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    ...logistical, like briefly explaining a gap in your job history or sharing relocation plans. They can be skills-based...cover letters for entry-level positions, as at that stage, cover letters can be a barrier for qualified applicants...

  16. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    Published
    Nov. 15, 2017, 3 p.m.
    ...wild-type AAV2 stocks (Grimm et al., 1999). The percentage of genome-containing viral capsids is typically...Quantitative PCR (qPCR) Digital droplet PCR (ddPCR) Historically, a quantitative DNA hybridization method (DNA...

  17. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    Published
    Nov. 1, 2018, 12:35 p.m.
    ...that retain the ccdB gene die off and a higher percentage of transformants contain the ccdB-less construct...PMID: 7768854. PubMed Central PMCID: PMC177048. Thisted, Thomas, and Kenn Gerdes. "Mechanism of post-segregational...

  19. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...GAL4 gene downstream of a tissue- or developmental stage-specific promoter and design a UAS reporter construct... GAL80 under different promoters and create sophisticated patterns of UAS-driven gene expression. To further...

  20. Finding Your Science Policy Path

    Type
    Blog Post
    Published
    Sept. 22, 2020, 1:15 p.m.
    ...an account and start engaging with the #scipol hashtag. Often there will be posts about virtual meet ups...move into science policy. Read broadly about the historical roots of science policy to connect to your why...
Showing: 201 - 220 of 223 results