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We narrowed to 809 results for: nin

Showing: 551 - 600 of 809 results

  2. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    Published
    Dec. 3, 2020, 2:15 p.m.
    ...serotypes that have thus far been identified: AAV1-11. Nine regions of variability in the cap gene which differ...334 . https://doi.org/10.1007/s40259-017-0234-5  Büning H, Srivastava A (2019) Capsid Modifications for...

  3. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

    Type
    Blog Post
    Published
    Jan. 21, 2020, 2:30 p.m.
    ...and annotated from more than 45 publications, beginning with the seminal research articles that demonstrated...Addgene blog Read this blog post about genome-wide screening using CRISPR Find all of our CRISPR pooled library...

  4. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    Published
    May 18, 2023, 1:15 p.m.
    ..., our antibodies have induced “stabbing” and “poisoning,” but let’s be honest, the elimination style the... as phagocytosis.  Other functions We’re still learning about Fc effector functions and all that antibodies...

  5. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    Published
    Dec. 14, 2020, 2:15 p.m.
    ...showing the effectiveness of using TRUPATH as a screening technique. Lastly, the activity of both antagonists...plasmids are available as a collection, facilitating screening across the transducerome, to gain a more complete...

  6. Management for Scientists: Giving Feedback

    Type
    Blog Post
    Published
    Feb. 6, 2015, 2:46 p.m.
    ...bring about change in behaviors and habits in a nonthreatening way. However, I have also found that scientists...interpretation of my feedback to you. What are We Learning: What would have happened if _________? What ...

  7. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    Published
    April 15, 2014, 3:11 p.m.
    ... precious in less resourced areas of the world.  Nina Dudnik, Founder of Seeding Labs, tells the story...bothered by the fact that I was wasting buffer running my tiny gels in a normal gel box. It took a bit...

  8. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    Published
    April 25, 2019, 3:01 p.m.
    ... which allows researchers to deposit plasmids containing the fluorescent protein mNeonGreen. This fluorescent...suited for certain studies. If you have plasmids containing mNeonGreen, mTFP1, or mWasabi that you would ...

  9. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    Published
    Jan. 24, 2019, 2:24 p.m.
    ...Figure 2). We’ve also created constructs with containing chemical tags such as the SNAP-tag, Halo-tag,...17972876. Kohl, Johannes, et al. "Ultrafast tissue staining with chemical tags." Proceedings of the National...

  10. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    Published
    Nov. 17, 2016, 3:30 p.m.
    ...undergo rigorous quality control testing at Addgene meaning they come ready made to accelerate your research...both chemo- and optogenetics. We’re therefore beginning our AAV viral services with these tools. A variety...

  11. Plasmids 101: Origin of Replication

    Type
    Blog Post
    Published
    Nov. 11, 2020, 2:23 p.m.
    ...CDF) D Relaxed This table defines common cloning vectors, their copy number, ori, and incompatibility...are especially useful to keep in mind if you are planning to purify your plasmid DNA: The insert Bacteria...

  12. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    Published
    March 28, 2022, 4:30 p.m.
    ...experiments directly from Addgene. How Addgene is Reimagining Antibody Sharing Addgene is building our antibody...well.     References Andrews NP, Boeckman JX, Manning CF, Nguyen JT, Bechtold H, Dumitras C, Gong B, ...

  15. Viral Vectors 101: Transductions

    Type
    Blog Post
    Published
    April 30, 2024, 1:15 p.m.
    ...time to make virus! The constructed viral vector containing the cargo of interest is then typically transfected...transduced (like a rogue HEK-293 cell). This virus-containing media can then be placed on whatever cells you...

  17. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    Published
    Sept. 15, 2015, 2:30 p.m.
    ...] is pretty great for us,” Wang said. “We were planning on and looking at a bunch of different strategies...mutations in the gene may prevent neurons from returning to basal conditions normally after periods of ...

  18. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    Published
    Aug. 25, 2015, 2:30 p.m.
    ... like to share my own experiences founding and running miLab to describe some key lessons I learned while... upon these people for guidance.  Before even beginning the process of launching a company, you need to...

  20. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    Published
    May 6, 2014, 1:07 p.m.
    ...calcium and other small molecules since the very beginnings of the field. Those engineered sensors were designed... began by isolating a calcium-binding protein, troponin C (TnC), from muscle cells. They identified a ...

  21. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    Published
    Sept. 12, 2019, 1 p.m.
    ...Since the beginning of our viral service in 2016, we’ve added many new tools to our inventory of ready-to-use... lowering sensor resting fluorescence, while maintaining high peak fluorescence) and is useful for imaging...

  23. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    Published
    June 18, 2019, 1:15 p.m.
    ...Since the beginning of our viral service in 2016, we’ve added many new tools to our inventory of ready-to-use... for interpreting the results of studies. When planning AAV experiments, scientists often have to choose...

  24. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    Published
    May 21, 2019, 12:10 p.m.
    ...retina. This pattern was consistent with TUNEL staining, a popular method to observe apoptotic cells. ...Central PMCID: PMC5026494. Zhang, Qiang, et al. "Designing a green fluorogenic protease reporter by flipping...

  25. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    Published
    Dec. 20, 2018, 1:15 p.m.
    ...remembered kindly. However, despite many headlines questioning if He’s experiments prove otherwise, the scientific...biosecurity, and the entire 2018 saga of “what’s happening in genetics this time?” She lives with her partner...

  26. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    Published
    Aug. 2, 2018, 12:56 p.m.
    ...PhD student at MIT. While I had always enjoyed learning about biology in high school, it wasn’t until ...experiences and knowledge with each other to help make learning biology more fun – bit by bit. Ally Huang is ...

  27. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ...laboratory for citizen scientists. In addition to maintaining a laboratory to support both individual and community...that everyone gets proper equipment and safety training prior to getting started. For example, Timothy...

  29. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    Published
    March 19, 2020, 1:15 p.m.
    ...while listening to podcasts made for kids. Plan out meals and snacks ahead of time.  Find learning activities...

  30. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    Published
    Feb. 9, 2021, 2:15 p.m.
    ...to chat. This can be done randomly or manually assigning which participants to group together. Breakout...doing science, he has assisted many researchers in gaining the confidence to launch new and diverse careers...

  31. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    Published
    Jan. 12, 2021, 2:15 p.m.
    ...relationships Here are some ways to go about strengthening a new relationship with authenticity and not...(or better yet, become a volunteer). Naturally running into or working with the same people is often the...

  32. How to Lead a Great Meeting

    Type
    Blog Post
    Published
    Feb. 9, 2016, 3:30 p.m.
    ...hour to achieve the purpose of the meeting (see “defining the scope” above) or if you can cut it down to...leader for some tips. Before long, you will be running collaborative, informative, energizing meetings...

  33. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    Published
    Dec. 11, 2018, 12:46 p.m.
    ...Developing science communication skills, while also learning about new scientific developments and helping ...s advice to tap into the online conversations happening around a scientific conference, whether you are...

  35. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    Published
    July 19, 2018, 1:12 p.m.
    ...bootstrap your science communication skills with training programs, which are increasingly available. But... draw and the perspective I want them to form? Training in science communication should prepare you to...

  36. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    Published
    May 25, 2017, 2:30 p.m.
    ...tools, where light used for excitation can cause unintended activation of the optogenetic system. Biosensors... cellular physiology in your system. Optical sectioning cannot be conducted with luminescence imaging...

  38. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    Published
    March 23, 2017, 2:30 p.m.
    ...Institute have supported the AAV initiative from the beginning and have worked closely with us to functionally... isn’t the only facet of a project this big. Maintaining our excellent service and accuracy involves caring...

  40. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    Published
    Feb. 1, 2022, 2:15 p.m.
    ...subject to mishandling?  Is the experiment you’re planning to run with the antibodies time-sensitive, using...which you may need in your experiment.  If you are planning to conjugate your antibody in the lab, you’ll ...

  41. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    Published
    Jan. 25, 2024, 2:15 p.m.
    ...you are in the design phase of your experimental planning, you need more nitty-gritty details — how can ...link to search Addgene's collection for plasmids containing that enzyme, and embedded in the References lists...

  42. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    Published
    March 21, 2023, 1:15 p.m.
    ...manipulate what this cargo is and where it goes, turning these pesky germs into useful tools. Here, we will...Harbor, NY, USA: 1997. PubMed PMID: 21433340. 4. Cronin J, et al. “Altering the tropism of lentiviral vectors...

  43. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    Published
    June 6, 2024, 1:50 p.m.
    ...When you are running flow cytometry, you’ll need various controls to help you set up and analyze your...and be in the same fluorophore (e.g., if you are staining for anti-mouse Ly6C in APC with the IgG2c subclass...

  45. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    Published
    June 2, 2016, 2:30 p.m.
    ...command line, and so was really starting from the beginning. Luckily, a fellow graduate student and a data... entered is useful for beginners who are still learning the basics. However, these notes take up a lot...

  46. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    Published
    May 22, 2014, 4:02 p.m.
    ... for publication and is, in most cases, blind (meaning you don’t know who the reviewers are). If the reviewers...future. Disregard reviewer’s comments without explaining why. If it looks like you have ignored their ...

  47. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    Published
    Oct. 1, 2019, 1:21 p.m.
    ...Do I have to be on LinkedIn?” For anyone who is planning or might need to plan for a career outside academia...hiring managers will think it is odd. For those planning a career in academia, it may not be required, ...

  49. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ...interactions, Ramanathan et al. designed an RNA bait containing three well conserved but not well characterized... and post-transcriptional regulation of SM1v1-containing RNAs could play a role in the severity of breast...

  50. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ... additionally provides detailed information on cloning procedures used to construct the deposited plasmids...Considerations when getting the dTAG system up and running in your lab Confirm the functionality of your fusion...
Showing: 551 - 600 of 809 results