We narrowed to 293 results for: gne
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TypeBlog PostPublishedAug. 28, 2025, 1:15 p.m.... Huang, H., et al. (2025). ATLAS: a rationally designed anterograde transsynaptic tracer. Nature Methods...
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Degrading DNA with Cascade-Cas3
TypeBlog PostPublishedFeb. 11, 2025, 2:15 p.m....maximum efficiency of the process. Various labs have designed all-in-one Cascade plasmids — such as the Tomoji... -
Hot Plasmids and Viral Preps - May 2021
TypeBlog PostPublishedMay 18, 2021, 1:15 p.m....the mutated spike protein and a barcode, and is designed so that the SARS-CoV-2 spike RBD is displayed ... -
Plasmids 101: Optimizing Plasmid Yields
TypeBlog PostPublishedMay 26, 2016, 2:30 p.m..... Spiking your media with supplements such as magnesium salts, buffering agents, and/or supplying additional... -
When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells
TypeBlog PostPublishedApril 19, 2016, 2:30 p.m....of environmental changes that the biosensor is designed to detect resulting in false positives [2, 4].... -
Anatomy of a Plasmid Page at Addgene
TypeBlog PostPublishedFeb. 4, 2016, 3:30 p.m....is a brief description of what the plasmid was designed and used for in the depositing lab, although you... -
Genome-wide Screening Using CRISPR
TypeBlog PostPublishedAug. 18, 2015, 2:30 p.m.... the genome (see Fig. 2). Individual gRNAs are designed in silico using publicly available gRNA design... -
Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards
TypeBlog PostPublishedMay 22, 2015, 4:58 p.m....one mistake can be enough. Reversal drives are designed to overwrite a previous gene drive and thereby... -
Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments
TypeBlog PostPublishedSept. 23, 2014, 3:05 p.m....the site. Like Labguru, Quartzy offers software designed with scientists workflow in mind and can offer... -
Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More
TypeBlog PostPublishedJune 12, 2014, 3:30 p.m....resistant to disease. They also devise biosensors designed to elucidate the molecular interactions that go... -
Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection
TypeBlog PostPublishedDec. 12, 2013, 3:35 p.m....systems with quantitative precision. There are few designers of biological systems today who are building 10... -
MXS Chaining
TypeBlog PostPublishedFeb. 7, 2017, 3:30 p.m....Origin and purpose of MXS chaining MXS-chaining was designed to create plasmids for fluorescence imaging or... -
The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data
TypeBlog PostPublishedJuly 16, 2020, 1:15 p.m....are no exception to this effect -- meticulously designed experiments in labs around the world were put ... -
Great Results Start with Great Standard Curves
TypeBlog PostPublishedAug. 15, 2024, 1:15 p.m....often recommend using advanced graphing software designed to plot complex trendlines. If you don’t have ... -
DNA Purification Without a Kit
TypeBlog PostPublishedFeb. 11, 2020, 2:15 p.m....RNA can be amplified. Compared to a commercial magnetic bead-based kit, this cellulose-based method was... -
Better Dyeing Through Chemistry & Small Molecule Fluorophores
TypeBlog PostPublishedSept. 8, 2016, 2:30 p.m....spectral range. Nevertheless, these fluorophores were designed as antibody and oligonucleotide labels for fixed-cell... -
Lentiviral Vector Uses and Overview
TypeBlog PostPublishedMay 19, 2016, 2:30 p.m.... knockout cell lines. Many CRISPR plasmids are designed for lentiviral use, including Addgene’s top requested... -
CRISPR Antimicrobials
TypeBlog PostPublishedMay 3, 2016, 2:30 p.m....currently undergoing a renaissance. Yosef et al. designed a two-phage CRISPR system to resensitize antibiotic-resistant... -
Cpf1: A New Tool for CRISPR Genome Editing
TypeBlog PostPublishedOct. 14, 2015, 2:30 p.m....to examine Cpf1 off-target editing. First, they designed gRNAs with single and double mismatches throughout... -
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)
TypeBlog PostPublishedMay 15, 2014, 3:33 p.m....array of GFP-based fluorescent biosensors has been designed to detect a variety of intracellular conditions...