We narrowed to 273 results for: phy

1. Better Dyeing Through Chemistry & Small Molecule Fluorophores

   Type

   Blog Post

   Published

   Sept. 8, 2016, 2:30 p.m.

   ...give you the best of both worlds: the excellent photophysics of chemical dyes combined with the ease and ...

2. CRISPR Antimicrobials

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   Blog Post

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   May 3, 2016, 2:30 p.m.

   ...Bacterial Genome Engineering Treating Muscular Dystrophy with CRISPR Gene Editing Using CRISPR to Edit ...

3. Tips for Screening with Yeast Two Hybrid Systems

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   Blog Post

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   Oct. 22, 2015, 2:30 p.m.

   ...that the configuration of your fusion proteins physically blocks the binding sites for the protein partner...

4. Plasmids 101: Protein tags

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   Blog Post

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   Dec. 11, 2014, 4:26 p.m.

   ...proteins can be purified using metal-affinity chromatography, usually using a Ni2+ column. Like other affinity...

5. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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   Blog Post

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   Dec. 2, 2014, 7:23 p.m.

   ...enChIP) using CRISPR. Fujita T, Fujii H. Biochem Biophys Res Commun. 2013 Aug 11. pii: S0006-291X(13)01329...

6. Your Lentiviral Plasmid FAQs Answered

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   Blog Post

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   April 23, 2014, 1:08 p.m.

   ...differences may seem subtle they result in a key physiological difference between lenti- and retroviruses. ...

7. Twenty Years of Sharing: Addgene's Viral Vector Service

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   Blog Post

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   July 30, 2024, 1:15 p.m.

   ...Janelia Research Campus on the GENIE website.   Bibliography  Ersing, I., Rego, M., Wang, C., Zhang, Y., ...

8. Viral Vectors 101: Preparing Pooled Libraries

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   Blog Post

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   July 13, 2023, 1:15 p.m.

   ...can transduce your cells.  While Addgene uses a physical titer (via ddPCR) to quality control our viral...

9. Viral Vectors 101: Pseudotyping

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   Blog Post

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   April 20, 2021, 2:30 p.m.

   ...Malone KD, Wolf CR, Chu HY, Tortorici MA, Veesler D, Murphy M, Pettie D, King NP, Balazs AB, Bloom JD (2020...

10. CRISPR-based Genome Editing Systems For Editing in E. coli

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    Blog Post

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    April 13, 2021, 1:15 p.m.

    ...editing in Escherichia coli. Acta Biochimica et Biophysica Sinica. https://doi.org/10.1093/abbs/gmab036 ...

11. Plasmids 101: Plasmid Incompatibility

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    Blog Post

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    March 31, 2020, 1:15 p.m.

    ...molecules, forming a “handcuff” structure that physically blocks replication machinery when plasmid copy...

12. New CRISPR Tools: Cas7-11 and PASTE

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    Blog Post

    Published

    Feb. 17, 2022, 2 p.m.

    ...originally suspected that this new Cas7-11 might be phylogenetically classified as the more complex Class I systems...

13. How to Write a Scientific Review Article

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    Blog Post

    Published

    Feb. 16, 2017, 3:30 p.m.

    ...histone deacetylases in B-and T-cell development, physiology and malignancy." Genes & cancer 6.5-6 (2015):...

14. Antibiotic Resistance: An Old Solution but a New Problem

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    Blog Post

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    Nov. 10, 2016, 3:30 p.m.

    ...the risk to others. Your friends, family, and physicians will thank you! Many thanks to our Guest Blogger...

15. Using Video to Share Your Science: We Share Science

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    Blog Post

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    Nov. 3, 2016, 2:30 p.m.

    ...research abstracts. With journals in medicine, physics, math, and a few other disciplines leading the ...

16. Changing Labor Laws Bring Increased Postdoc Wages

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    Blog Post

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    Oct. 25, 2016, 2:30 p.m.

    ...changes in science. Follow him on Twitter @BiophysicalFrog and Future of Research @FORsymp.   Additional...

17. Getting the Most from Your Lentiviral Transduction

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    Blog Post

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    April 7, 2016, 2:30 p.m.

    ...translated to other lines. Differences in the physiology of different cell types, however, can make this...

18. Tips for Using FRET in Your Experiments

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    Blog Post

    Published

    Nov. 5, 2014, 3:41 p.m.

    ...Periasamy defined FRET as "a distance-dependent physical process by which energy is transferred non-radiatively...

19. Plasmids 101: The Promoter Region – Let's Go!

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    Blog Post

    Published

    April 3, 2014, 8:05 p.m.

    ...consistent expression regardless of cell type or physiology. SV40 General expression mRNA Mammalian expression...

20. Transferable Skills Guide: Time Management

    Type

    Blog Post

    Published

    Dec. 5, 2017, 1:52 p.m.

    ...down? It’s more viscerally appealing for me to physically cross things out (it just feels good) and I remember...