We narrowed to 414 results for: vent

1. What's the Best Way to Elute and Store Your Plasmid DNA?

   Type

   Blog Post

   Published

   June 4, 2020, 1:22 p.m.

   ...EDTA chelates any divalent cations like Mg2+, preventing the activity of DNase. While this is very important...

2. Fostering communication in remote collaborations

   Type

   Blog Post

   Published

   April 21, 2020, 1:14 p.m.

   ...This also accelerates the pace of research by circumventing the slower, sequential communication of emailing...

3. Sensing Neuronal Dopamine

   Type

   Blog Post

   Published

   July 30, 2019, 12:59 p.m.

   ...dopamine and other biosensors in our AAV vector inventory. References Darvesh, Altaf S., et al. "In vivo...

4. CASTing Off for New Shores in Human Genome Editing

   Type

   Blog Post

   Published

   June 10, 2025, 1:15 p.m.

   ... that can deliver a healthy gene as a single intervention for a variety of loss-of-function mutants could...

5. Build Your CRISPR Vocabulary

   Type

   Blog Post

   Published

   July 2, 2024, 1:15 p.m.

   ...require a tracrRNA. sgRNA: “Single-guide RNAs” were invented by synthetic biologists. An sgRNA combines a crRNA...

6. Antibodies 101: Affinity Tags

   Type

   Blog Post

   Published

   Sept. 19, 2023, 1 p.m.

   ...chromatography requires proper folding of the tag, which prevents insoluble fusion proteins from being purified ...

7. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

   Type

   Blog Post

   Published

   June 29, 2023, 7 p.m.

   ...taking for protocol video production, and keeping inventory of our merchandise collection. While most days...

8. Management for Scientists: Giving Feedback

   Type

   Blog Post

   Published

   Feb. 6, 2015, 2:46 p.m.

   ...let things fester, give feedback as close to an event as possible. If you wait too long, a small correction...

9. The Stingy Scientist: How the Baby Gel Box Was Born

   Type

   Blog Post

   Published

   April 15, 2014, 3:11 p.m.

   ...biologists used this procedure daily before the advent of techniques like PCR and DNA synthesis. Most ...

10. Plasmids 101: Antibiotic Resistance Genes

    Type

    Blog Post

    Published

    Jan. 30, 2014, 3:29 p.m.

    ...directly kill bacteria (bactericidal) or slow growth/prevent cell division (bacteriostatic); however, the distinction...

11. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

    Type

    Blog Post

    Published

    June 27, 2018, 1:16 p.m.

    ... camelids. They possess many advantages over conventional antibodies: they are small (12-15 kDa), stable...

12. Science Careers: Unruly Interests Feed Many Paths

    Type

    Blog Post

    Published

    Sept. 26, 2017, 12:12 p.m.

    ...galaxy. TL;DR: Many roads need scientists open for adventure. Many thanks to our guest writer, Pamela J. Hines...

13. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type

    Blog Post

    Published

    June 2, 2020, 1:15 p.m.

    ...avoid a high false-positive rate discovery rate, conventional CRISPR screens require a low multiplicity of...

14. Meet the New Addgene Board Members!

    Type

    Blog Post

    Published

    April 28, 2020, 1:14 p.m.

    ...over 100 requests for the plasmids and the lab eventually deposited these plasmids at Addgene. While deposits...

15. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type

    Blog Post

    Published

    Oct. 20, 2020, 1:15 p.m.

    ... is good for everyone including those that find events stressful. Video chatting makes it pretty easy ...

16. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type

    Blog Post

    Published

    Aug. 11, 2020, 1:15 p.m.

    ...complete ITR loss abolishing AAV packaging. To prevent ITR loss, it’s best to propagate AAV constructs...

17. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type

    Blog Post

    Published

    Jan. 25, 2018, 2:09 p.m.

    ...step and the transformed bacteria refuse to grow. Eventual success was achieved by inserting BirA into a ...

18. The Golden Stopwatch Award: Recognizing Facilitators of Research

    Type

    Blog Post

    Published

    Sept. 5, 2019, 1:06 p.m.

    ...Transfer: the exchange of intellectual property (as inventions, ideas, plasmids) among universities and other...

19. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

    Type

    Blog Post

    Published

    Aug. 8, 2019, 1:19 p.m.

    ...inconsistencies in the work, missing information that would prevent the study to be replicated or omission from the...

20. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type

    Blog Post

    Published

    Jan. 24, 2019, 2:24 p.m.

    ...vector-mediated gene delivery is more common than conventional transgenic strategies. In such vector-based ...