Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 729 results for: NSI

Showing: 121 - 130 of 729 results

  1. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ...isotype choice. Target and application should be considered The first question you may have is whether a ...monoclonal primary antibody (A). To boost signal, consider using subclass specific secondary antibodies instead...immunoprecipitations. Your sample species should also be considered when choosing an antibody. In some applications...options are available to you, it may be worth considering an antibody raised in a different species.    ...      Table 1: Isotype considerations when choosing antibodies   Complexity is a key factor...with a distinctly conjugated secondary antibody. Consider too that some secondary antibodies can cross react...

  2. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ...Your time is precious. Here are a few things to consider before you commit: Double check to make sure ...can be a particularly good resource if you are considering using a reagent for “off-label” purposes. When...about using Google for serious science questions, consider using Google Scholar, which searches scientific...email you’re writing is probably not directly responsible for the problem. We’re here to help. You've ... few days without getting a response, however, consider following up. Ask for a timeline if you are ...upfront how long it will take to get an answer, consider asking to avoid unrealistic expectations. And...

  3. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ...details on how they work!) Figure 1: Base transitions are edits between the purine bases (adenine, ...limitation to this approach. CBEs and ABEs are base transition editors. They adjust the side groups of DNA bases...task, not a job for a single enzyme. Base transitions Base transversions C → TG → A A → GT → C ...some cases, the same repair pathways that were considered problematic in the development of CBEs and ABEs.... Like other ABEs, the editor they were using consisted of Cas9 fused to an engineered adenosine deaminase...rapidly. If it follows a similar path to base transition editing, we can expect several generations of...to repair DSBs. Base Editing terms   Base transition Base changes between purines (A ↔ G) or between...

  4. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...infects host cells through binding the human angiotensin converting enzyme 2 (ACE2) cell-surface receptor...receptor. The spike protein RBD responsible for binding ACE2 is the target of most anti-SARS-CoV-2 antibodies..., homogeneous time resolved fluorescence, and suspension bead assay (Figure 2). After finding no cross-reactivity...scFVs to cognate aaRS antigens as measured by suspension bead assay. Image from  Preger et al., 2020. ... a compact Cas9 ortholog from Staphylococcus lugdunensis that recognizes a NNGG PAM and has comparable...expressing GCaMP6f with this promoter. Image calcium transients in PV interneurons with AAV-S5E2-GCaMP6f are ...

  5. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    Published
    Sept. 7, 2017, 12:17 p.m.
    ...solution against a part of the body very sensitive to heat- the inside of the wrist or the cheek. (Parents ...work with, require extra serum to survive at low densities - adding to the expense of dilution cloning. ... transfected cells into 3 plates at different densities for selection). A 2% agarose solution is then ...plates containing selection agent at different densities, then dispose of the plates that do not appear...such as the Viaflo from Integra Biosciences) to transition from a 48-well cell plate to a 96-well assay ...

  6. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...class of FPs, the FP timers, add an entire new dimension to monitoring localization; using FP timers, researchers...production of dsRed E5. This timer predictably transitions from green to red fluorescence (500 to 580 nm...However, this initial timer was a tetramer with a propensity to aggregate in cells (Tsuboi et al.), limiting... in Xenopus embryos. Of course, timers may be sensitive to changes in oxygen, temperature, and pH, but...et al. developed monomeric FP timers with less propensity to aggregate. These monomeric FP timers (mK-GO... that differentiates human IPSCs into glucose-sensitive insulin-secreting beta-like cells." Nature communications...

  7. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ...is its simplicity and versatility. CRISPR/Cas9 consists of two components: the non-specific endonuclease...libraries (referred to simply as “CRISPR libraries”) consist of a heterogeneous population of gRNA-containing... is right for you There are several factors to consider when selecting a CRISPR library for your experiments...identified using next-generation sequencing (E). Considerations and tips for successful screens Next-generation...check out Addgene’s 2016 publication “Practical Considerations for Using Pooled Lentiviral CRISPR Libraries...Waxmonsky NC, Swanson LE, Fan M (2016) Practical Considerations for Using Pooled Lentiviral CRISPR Libraries...

  8. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ...As a person with many roles and responsibilities, I have to think a lot about how to balance those roles...meeting and a means to request funding for events, consider reaching out to it. PTAs have more flexibility...schooler himself and b) I had the background to be considered an authority in this area. What is genome engineering...presentation, she said “it was such a valuable extension to our curriculum and offered engaging examples...Educational Progress (NAEP), virtually none were considered proficient in science, regardless of race or ...posts Resources on Addgene.org Check out our comprehensive CRISPR guide Find CRISPR plasmids and resources...

  9. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ... blog post discusses some of the key things to consider when performing colony PCR.   Designing colony...for the presence of many different inserts. The downside: this type of primer does not provide information...information about insert orientation, then you might consider designing orientation-specific primers. Blunt ...day, you can save the leftover bacteria-water suspension and use them to start cultures of your positive...up PCR reactions: The remaining bacteria-water suspension will serve as the template for your PCR reaction...tool to have in your molecular biology tool kit. Consider giving it a try next time you’re screening for...

  10. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

    Type
    Blog Post
    Published
    Dec. 14, 2017, 2:08 p.m.
    ...cells, thus expressing additional EBNA-1 transiently. The transiently expressed EBNA-1 increases protein expression...field [4]. Three major reprogramming methods are considered when generating hiPSCs for clinical purposes:...purposes: mRNA transfection- this allows transient expression of the reprogramming factors Sendai virus- a non-integrating...efficiently [5]. However, the Sendai virus method is expensive, especially if used to make clinical grade hiPSCs...OCT3/4 and shRNA against p53 pCXWB-EBNA1 37624 transient EBNA-1   Many thanks to our guest blogger,...
Showing: 121 - 130 of 729 results