We narrowed to 293 results for: gne
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TypeBlog PostPublishedApril 23, 2014, 1:08 p.m....those seen when using a plasmid backbone that was designed specifically for transient expression (find some...
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Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design
TypeBlog PostPublishedSept. 13, 2018, 1:55 p.m....included in the library. Safe-targeting guides were designed to target genomic sites with no annotated function... -
Analyzing CRISPR Editing Results with ICE from Synthego
TypeBlog PostPublishedMay 8, 2018, 1 p.m....viewing a multiplex sample, the cut site will be aligned to the most 5’ cut site. Indel Distribution... -
What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program
TypeBlog PostPublishedMay 2, 2019, 12:41 p.m....promotes scientific sharing The EDGE Program is designed to expand the number of systems that can be studied... -
Hot Plasmids - August 2020
TypeBlog PostPublishedAug. 18, 2020, 1:15 p.m.... adsorption chromatography (IMAC) using Ni-NTA magnetic beads. Workflow of nanobody expression and... -
Plasmids 101: Plasmid Incompatibility
TypeBlog PostPublishedMarch 31, 2020, 1:15 p.m.... Environmental Plasmids blog). Scientists have designed small, high copy incompatible plasmids that lead... -
Twenty Years of Sharing: Expanding Accessibility
TypeBlog PostPublishedNov. 21, 2024, 10:47 p.m....all 101s on a particular topic into beautifully designed and easy-to-reference eBooks. Figure 3: Global... -
Hot Plasmids: Fall 2024
TypeBlog PostPublishedNov. 21, 2024, 1:30 p.m....specifically for C. elegans (Cao et al., 2024). The team designed coding sequences for mScarlet3 and mScarlet-I3... -
Twenty Years of Sharing: Addgene's Viral Vector Service
TypeBlog PostPublishedJuly 30, 2024, 1:15 p.m....early engagement with UPenn to our launch of the redesigned Data Hub in 2022. It’s influenced the way we ... -
Hot Plasmids Spring 2024
TypeBlog PostPublishedMay 23, 2024, 1:15 p.m....separation of the multiplexed elements. The team designed sgRNAs that include an internal barcode and separated... -
New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression
TypeBlog PostPublishedJan. 24, 2023, 2:15 p.m....Going Deeper: Biomolecular Tools for Acoustic and Magnetic Imaging and Control of Cellular Function. Biochemistry... -
Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research
TypeBlog PostPublishedJuly 29, 2025, 1:15 p.m.... Vectors The vectors pK-TnpB1 and pK-TnpB2 are designed for plant genome editing using TnpB. The main ... -
An Inside Look into Addgene's Dev Team
TypeBlog PostPublishedJan. 23, 2020, 2:45 p.m....code and have each developer work only on their assigned area. It’s natural that some of us have more in-depth... -
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs
TypeBlog PostPublishedDec. 14, 2017, 2:08 p.m....the CytoTune™-iPS 2.1 Sendai Reprogramming Kit designed to generate clinical grade hiPSCs is sold at $11,000... -
Career Insights: Technical Support Specialist
TypeBlog PostPublishedNov. 9, 2017, 2 p.m....easier. You should also demonstrate a strong willingness to help others with their problems, a general... -
Plasmids 101: Control Plasmids
TypeBlog PostPublishedApril 9, 2015, 3:29 p.m....controls are specific to the experiment and should be designed accordingly. If you are trying activate a gene... -
Replacing Paper: Tips for Choosing an Electronic lab Notebook
TypeBlog PostPublishedJuly 10, 2018, 1:08 p.m.... flexible generic solution, rather than an ELN designed for a specific research area, due to anxieties... -
Adeno-associated Viruses (AAVs) for Genome Editing
TypeBlog PostPublishedMarch 27, 2018, 1:32 p.m....human cells (Kim et al, 2016). The Waldman lab has designed their AAV tagging vectors (pAAV-SEPT-Acceptor ... -
Deep Mutational Scanning with One Pot Saturation Mutagenesis
TypeBlog PostPublishedFeb. 22, 2017, 3:30 p.m.... during the selection process. Each variant is assigned a functional score based on its library frequency... -
Harnessing Bacterial Toxins for Allelic Exchange
TypeBlog PostPublishedAug. 15, 2019, 12:30 p.m....the pDS132 MCS with a versatile, synthetic MCS (designed with the help of the DNA tuner program) (Latynski...