We narrowed to 414 results for: vent

1. In Vivo Biotinylation of Bacterial Fusion Proteins

   Type

   Blog Post

   Published

   Jan. 25, 2018, 2:09 p.m.

   ...step and the transformed bacteria refuse to grow. Eventual success was achieved by inserting BirA into a ...

2. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

   Type

   Blog Post

   Published

   June 2, 2020, 1:15 p.m.

   ...avoid a high false-positive rate discovery rate, conventional CRISPR screens require a low multiplicity of...

3. Meet the New Addgene Board Members!

   Type

   Blog Post

   Published

   April 28, 2020, 1:14 p.m.

   ...over 100 requests for the plasmids and the lab eventually deposited these plasmids at Addgene. While deposits...

4. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

   Type

   Blog Post

   Published

   Jan. 24, 2019, 2:24 p.m.

   ...vector-mediated gene delivery is more common than conventional transgenic strategies. In such vector-based ...

5. 10 Great Guest Posts We're Thankful For

   Type

   Blog Post

   Published

   Nov. 18, 2016, 3 p.m.

   ...and/or engineer “temporally ordered biochemical events” such as those that occur during development. Read...

6. Plasmids 101: TOPO Cloning

   Type

   Blog Post

   Published

   Oct. 27, 2016, 2:30 p.m.

   ...rebind and nick the newly ligated DNA. Salt helps prevent topoisomerase I from rebinding, which results in...

7. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

   Type

   Blog Post

   Published

   July 7, 2015, 3:36 p.m.

   ...few hundred worms, making the detection of rare events difficult. Dickinson et al. sought to create a ...

8. Another Pathway into Cells: iTOP

   Type

   Blog Post

   Published

   June 23, 2015, 8:37 p.m.

   ... expression would require multiple transduction events. For these reasons, iTOP is best suited to binary...

9. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

   Type

   Blog Post

   Published

   April 7, 2015, 4:21 p.m.

   ...the generation of various flux imbalances. The conventional approach has typically been to either systematically...

10. The MTA at Addgene

    Type

    Blog Post

    Published

    July 23, 2024, 2:17 p.m.

    ...meeting in 1990 to discuss the process, which eventually led to the creation of a standardized agreement...

11. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type

    Blog Post

    Published

    Feb. 13, 2024, 2:15 p.m.

    ... replicate the complexity of antigen processing events that take place within an antigen-presenting cell...

12. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type

    Blog Post

    Published

    March 30, 2023, 1:15 p.m.

    ...as a single mutation or expression dysregulation event. Diseases are often complex, with multiple layers...

13. Pushing the Limits of DNA Assembly

    Type

    Blog Post

    Published

    Sept. 6, 2017, 1:01 p.m.

    ...accurately execute. However, these challenges can be circumvented by generating individual fragments containing...

14. Visualizing Translation at the Single Molecule Level

    Type

    Blog Post

    Published

    Aug. 1, 2017, 1:15 p.m.

    ...the mRNA to the plasma membrane; this sequence prevents diffusion of the mCherry labeled mRNA and keeps...

15. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type

    Blog Post

    Published

    Feb. 9, 2021, 2:15 p.m.

    ...working, reconnect as human beings and colleagues, eventually encouraging purposeful and creative collaboration...

16. FPbase: A new community-editable fluorescent protein database

    Type

    Blog Post

    Published

    May 16, 2018, 1 p.m.

    ... management system (PostgreSQL), that aims to eventually be a comprehensive community resource for information...

17. What Good Citizenship Can Do for Reproducibility in Science

    Type

    Blog Post

    Published

    Nov. 7, 2019, 2:18 p.m.

    ...all your friends are racing around the board. Eventually, you get lucky and manage to escape, but you’...

18. Celebrate National Book Month with Addgene

    Type

    Blog Post

    Published

    Oct. 3, 2019, 12:47 p.m.

    ... their paths cross with each other and with the events of the times."     - Aliyah Weinstein, Marketing...

19. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type

    Blog Post

    Published

    May 21, 2019, 12:10 p.m.

    ...activation. Normally, β10-11 are antiparallel. To prevent binding, the β strands in FlipGFP were redesigned...

20. Reagent Repositories Are Speeding up Science During the Pandemic

    Type

    Blog Post

    Published

    Aug. 4, 2020, 1:15 p.m.

    ...science research, including over 4,000 cell lines. Seventeen of these cell lines were used to investigate the...