We narrowed to 293 results for: gne

1. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

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   Blog Post

   Published

   April 9, 2019, 1:13 p.m.

   ...consist of random amino acid sequences or (ii) designed to form an inert, unstructured peptide and therefore...

2. The Fluorescent Vegetables in Aptamer Soup

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   Blog Post

   Published

   Jan. 29, 2019, 2:27 p.m.

   ... intracellularly due to a lower dependency on magnesium and higher thermal stability, making it a potentially...

3. Replacing Paper: Tips for Choosing an Electronic lab Notebook

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   Blog Post

   Published

   July 10, 2018, 1:08 p.m.

   ... flexible generic solution, rather than an ELN designed for a specific research area, due to anxieties...

4. Five Popular Model Organisms, Part 2

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   Blog Post

   Published

   Sept. 29, 2020, 1:15 p.m.

   ...allows for easy cloning of constructs specifically designed for use in Arabidopsis. There are also important...

5. Harnessing Bacterial Toxins for Allelic Exchange

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   Blog Post

   Published

   Aug. 15, 2019, 12:30 p.m.

   ...the pDS132 MCS with a versatile, synthetic MCS (designed with the help of the DNA tuner program) (Latynski...

6. Plasmids 101: Expressing shRNAs for RNA Interference

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   Blog Post

   Published

   June 17, 2025, 1:15 p.m.

   ...effects. More recently, shRNA plasmids are being designed using RNA polymerase II promoters. The design ...

7. Antibodies 101: Beyond Surface Labeling

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   Blog Post

   Published

   June 27, 2024, 1:15 p.m.

   ...negatively selecting your cells of interest is magnetic bead-activated cell sorting, or MACS. But MACS...

8. New Optogenetic Tools for Cytoskeleton and Membrane Control

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   Blog Post

   Published

   Sept. 28, 2023, 1:15 p.m.

   ...optogenetics than neuronal activity. Researchers have designed a variety of photoswitchable elements into many...

9. Hot Biosensors 2022: Year-End Roundup

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   Blog Post

   Published

   Dec. 6, 2022, 2:15 p.m.

   ...genetically encoded temperature indicator B-gTEMP designed to meet those requirements (Lu et al. 2022). By...

10. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

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    Blog Post

    Published

    March 1, 2016, 3:30 p.m.

    ...assembly, long primers for each fragment must be designed and ordered, and are specific for each fragment...

11. Treating Muscular Dystrophy with CRISPR Gene Editing

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    Blog Post

    Published

    Jan. 26, 2016, 3:30 p.m.

    ... for Duchenne muscular dystrophy. gRNAs can be designed to flank a single or multiple exons that, when...

12. Plasmids 101: Common Lab E. coli Strains

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    Blog Post

    Published

    Nov. 7, 2014, 2:56 p.m.

    ...hundreds. This only includes general lab strains designed for subcloning or protein expression. If you were...

13. CRISPR 101: Epigenetics and Editing the Epigenome

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    Blog Post

    Published

    June 24, 2020, 5:45 p.m.

    ...certainly not the only CRISPR-based technology designed to alter gene expression. Fusing dCas9 to a transcriptional...

14. How to Design Your gRNA for CRISPR Genome Editing

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    Blog Post

    Published

    Sept. 24, 2020, 1:15 p.m.

    ...but it is important that your gRNA sequence is designed to be highly active and reduce off targets. For...

15. Viral Vectors 101: AAV Serotypes and Tissue Tropism

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    Blog Post

    Published

    Jan. 16, 2025, 2:15 p.m.

    ...., Schaffer, D. V., & Karpova, A. Y. (2016). A Designer AAV Variant Permits Efficient Retrograde Access...

16. Technique: Probe Phage Genomes for Host Binding Proteins

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    Blog Post

    Published

    Sept. 29, 2016, 2:30 p.m.

    ...ends) and ligate into a bluntly digested vector designed for protein overexpression in E. coli, such as...

17. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

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    Blog Post

    Published

    March 7, 2017, 3:30 p.m.

    ... amplified a 500-600 bp product. The primers I designed (listed above) worked well and I got clear bands...

18. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type

    Blog Post

    Published

    Dec. 17, 2020, 1:30 p.m.

    ... Ghanta KS, Doksin GA, Mir A, Krishnamurthy PM, Gneid H, Edraki A, Watts JK, Sontheimer EJ, Mello CC (...

19. Antibodies 101: Antibody Engineering and Directed Evolution

    Type

    Blog Post

    Published

    Aug. 5, 2025, 1:15 p.m.

    ... antibody sequences, degenerate primers can be designed to selectively mutate specific regions of your...

20. Live and Let Dye: Self-Labeling Protein Tags

    Type

    Blog Post

    Published

    April 16, 2024, 1:15 p.m.

    ... such as with substrate-linked PEG, biotin, or magnetic beads. In one innovative example, the Zhang lab...