We narrowed to 293 results for: gne

1. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

   Type

   Blog Post

   Published

   March 7, 2017, 3:30 p.m.

   ... amplified a 500-600 bp product. The primers I designed (listed above) worked well and I got clear bands...

2. Antibodies 101: Designing Your First Flow Panel

   Type

   Blog Post

   Published

   May 28, 2024, 1:45 p.m.

   ...avoid or reduce it whenever possible. Finally, we designed a simple panel to analyze transduced HEK 293T ...

3. Antibodies 101: Flow Cytometry

   Type

   Blog Post

   Published

   July 20, 2021, 1:15 p.m.

   ...based on intensity. Higher intensity events are assigned to higher channels. In a typical assay a user ...

4. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

   Type

   Blog Post

   Published

   June 28, 2018, 3:01 p.m.

   ...Dewari et al. 2018). Using the first crRNA we designed for ~250 genes, we have had about 30% tagging ...

5. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

   Type

   Blog Post

   Published

   April 25, 2017, 2:30 p.m.

   ...destroy most PA-FPs, but, in 2015, the Looger Lab designed 2 variants of EosFP that can withstand these conditions...

6. CRISPR 101: Cytosine Transversion Editors

   Type

   Blog Post

   Published

   March 25, 2025, 1:15 p.m.

   ...Zhou, R., Iyer, S., Garcia, S. P., Miller, B. R., Langner, L. M., Grünewald, J., & Joung, J. K. (2021). CRISPR...

7. A Needle in a Base-Stack: Cas9 Structural Biology

   Type

   Blog Post

   Published

   June 4, 2024, 1:15 p.m.

   ...Have you ever designed a CRISPR guide RNA and wondered why it is limited to only 20 bases, or why it’...

8. CRISPR 101: Multiplex Expression of gRNAs

   Type

   Blog Post

   Published

   Sept. 10, 2020, 11:45 a.m.

   ...also have the option of including a previously-designed tyr gRNA, which causes hypopigmentation, thus ...

9. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

   Type

   Blog Post

   Published

   Nov. 3, 2015, 3:30 p.m.

   ...-targeting, but currently no software has been designed for this purpose. This second area is extremely...

10. Deep Dive: qPCR

    Type

    Blog Post

    Published

    Sept. 29, 2022, 1:15 p.m.

    ...References and Resource section. Validation Once designed, your primer and probe set needs to be validated...

11. CRISPR 101: Cytosine and Adenine Base Editors

    Type

    Blog Post

    Published

    Feb. 13, 2025, 2:15 p.m.

    ...C. A., Garcia, S. P., Iyer, S., Miller, B. R., Langner, L. M., Hsu, J. Y., Aryee, M. J., & Joung, J. K...

12. 15 Hot Plasmids from 2017

    Type

    Blog Post

    Published

    Jan. 9, 2018, 3:02 p.m.

    ...codons) with kanamycin cassettes and were carefully designed to allow for easy removal of the resistance cassette...

13. Using AAV for Neuronal Tracing

    Type

    Blog Post

    Published

    Aug. 9, 2018, 1:04 p.m.

    ...., Schaffer, D.V., and Karpova, A.Y. (2016). A designer AAV variant permits efficient retrograde access...