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We narrowed to 729 results for: NSI

Showing: 151 - 160 of 729 results

  1. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...The light intensity used to activate the PA-FP should also be considered. If the light intensity needed ...your experiment, there are several parameters to consider, such as brightness, photostability, pH stability...protein aggregation. Another important parameter to consider is the contrast ratio- a comparison of PA-FP brightness...microscope will have it’s own quirks (level of zoom, intensity, possible excitation lasers) it is also important...

  2. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ... donor and acceptor fluorophores, it’s best to consider these cases separately when talking about the ...the tools that are available. Let’s consider the donor first where labeling specificity is most important...AnapRS Mammalian Cells Note: This is not an extensive list Acceptor labeling: Sometimes it's okay to... (i.e. >100 Å). Researchers have developed an extensive array of chemical labeling strategies suitable...understanding of your protein that can lead to novel insights about its biology and its behavior. See the table...covalent labeling of recombinant protein molecules inside live cells." Science281.5374 (1998): 269-272. PubMed...

  3. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    Published
    March 30, 2023, 1:15 p.m.
    ...from these sites. The Zhang lab generated a comprehensive library of each unique TF open reading frame...cellular fates were then matched with candidate TFs responsible for that differentiation.   Fig. 2: Single...Previously engineered TF libraries were not as comprehensive as this one, so it is an invaluable tool to ... the 24 TFs they tested, 22 expressed markers consistent with the cell type that was predicted based on... if the differentiated cells had morphologies consistent with the predicted cell type (e.g., axon-like...

  4. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    Published
    April 7, 2022, 1:15 p.m.
    ...conformational changes lead to an increase in fluorescence intensity from the inserted GFP. After characterizing iGluSnFR...by glutamate, and tweaking it to improve its responsiveness, they showed that iGluSnFR could detect neuronal...and produces stronger fluorescent signals upon sensing glutamate in mouse and ferret models. Furthermore...recently made iGluSnFR3 variants that are more sensitive and better localized for synaptic glutamate response... and produce stronger fluorescent signals upon sensing glutamate using both 1-photon and 2-photon imaging...

  5. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    Published
    May 27, 2025, 1:15 p.m.
    ...instructions and extensive references for troubleshooting. If you need a high level of consistency between lots...fall back to? We have outlined some important considerations to help you choose the plasmid prep kit that...between these manufacturers, as price is a large consideration for most labs. Many institutions have preferred...department for these.  While budget is an important consideration, you do want to make sure you’re getting the..., please note that Table 3 is certainly not comprehensive — there are many other vendors and kits out ...

  6. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    Published
    March 21, 2023, 1:15 p.m.
    ...can be engineered to be cell type specific. Once inside the host cell, the virus unleashes its cargo – ...signals which can traffic their viral particles inside the nucleus, without the need for envelope disassembly...viruses do. They are generally used to achieve transient, high expression of the relevant cargo without...Capacity (>8kb) High Transduction Efficiency Transient Gene Expression High Immunogenecity AAV ...Immunogenicity Infects Non-Dividing Cells Transient Gene Expression Low Tropism for Mouse Cells and...

  7. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    Published
    Feb. 8, 2022, 2:15 p.m.
    ...personalized steps like “check drawers for light-sensitive reagents” or “re-sterilize now.” Have a physical...Soon you’ll figure out what prompts you need to consistently and accurately run a protocol.   Figure...experiment than it is to rerun an experiment, so consider your prep an investment in accuracy.  Bench setup...hasn't. Developing simple habits like these can consistently reduce errors as you work, and the possibilities... to a protocol if you’re in a hurry, but let’s consider it this way: When you’re stressed or rushing, ...

  8. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    Published
    June 12, 2018, 11:40 a.m.
    ...DNA packed into our chromosomes is very much 3-dimensional, as you can see by this post’s chromatin illustrations...PIs, articles, genes, and blog posts are not comprehensive, so just because you do not see a name or gene...the gene you’re looking for on this list, please consider searching directly on our website. In-exhaustive...Reporter-nanobody fusions (RANbodies) as versatile, small, sensitive immunohistochemical reagents. Yamagata M, Sanes...chromatin and the epigenome. If you have been considering some new techniques, the posts below may steer...

  9. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type
    Blog Post
    Published
    Feb. 24, 2015, 7:50 p.m.
    .... The next insight was to assemble a synthetic transcription activation complex consisting of multiple...transcriptional activation of endogenous genes and it consists of three components: 1. A nucleolytically inactive...-mediated screens exhibited a high degree of consistency and validation with >80% effectively enriched...optimize humans for CRISPRi and CRISPRa.” Before considering the use of pooled libraries in your experiments...

  10. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    Published
    Feb. 18, 2014, 4:22 p.m.
    ...They all found the support of the group to be intrinsic to their success. Their stories made it clear ...programs. The groups in these programs typically consist of 1-2 mentors and 3-5 mentees who meet together... of people who are all interested in making a transition. Schedule your first meeting and get organized...in a rotation. The leader for that meeting is responsible for choosing a topic and leading the discussion... your successes and support each other as you transition to the next stage in your careers! Stay in touch...
Showing: 151 - 160 of 729 results