We narrowed to 821 results for: nin

... to improve viral vector production. Whether redesigning viral packaging plasmids, investigating different...

...Since the beginning of our viral service in 2016, we’ve added many new tools to our inventory of ready-to-use...

... 2). Induced cell death was confirmed by TUNEL staining.  The video below shows what happens to the zebrafish...

...her science communication skills throughout her training and wants to make her work understandable to people...

...are pooled together to create a single library containing fragments carrying sample-specific barcodes. ...

... this will give you an idea of what additional training you might need to obtain. You'll also need to ...

...with regards to SARS-CoV-2, this mouse strain is regaining popularity. The Jackson Laboratory, a nonprofit...

...many large conferences with multiple sessions happening at once, virtual conferences may have apps or ...

...these applications have lagged. Fixation, chemical staining, and light exposure can negatively affect FPs ...

...important roles microbes play in our ecosystem, examining relationships between microbes and insects, mammals...

...number changes) the original identifier loses its meaning, making it difficult or impossible for researchers...

...of CRISPR to correct single-gene conditions. Remaining hurdles in CRISPR biology “I think it is absolutely...

...beyond COVID-19 One year on, our biggest lesson running the platform has been the realization that there...

...phrase, such as being cautious, being critical, or defining terms. The phrases in Phrasebank are generic, ...

...but they are also the reason this research is happening. Stem cells scientists have certainly not lost...

...Cas9 breaks repaired by the non-homologous end-joining (NHEJ) pathway disrupt target gene sequences (thus...

... allow for episomal amplification of plasmids containing the viral EBV or SV40 ORIs, respectively. The...

...the activator protein can bind to the promoter, turning it ON and initiating transcription.     Negative...

...your experiment. You can see from Figure 2 that determining where to place your gate based on the dot plot...

...specific subcellular localization peptide. By combining three different localization peptides with four...

... Scientists have developed calcium indicators spanning nearly the entire visible spectrum (Figure 1)....

...visualized simultaneously. Complementation systems for examining protein-protein interactions Split luciferases...

... So how can you get involved? Keep asking your burning questions and tell us what you want to know more...

...PRESTO-TANGO (parallel receptorome expression and screening via transcriptional output - TANGO) (Kroeze et...

...event. Now it’s maybe one out of 100 or 50 event. Cloning of the second plasmid can be done in one day in...

...than you – meeting scientists at your level of training is always a good idea. Make contact with other...

...and semen samples. Ma et al. designed plasmids containing Cas9 and two different gRNAs, and electroporated...

...an affinity of 110 µM (Zacharias et al, 2002), meaning that at this concentration 50% of the protein forms...

...easy to identify: double-membraned structures containing undigested cytoplasmic contents. Autophagosomes...

...CRISPRainbow paper, Ma et al. engineered gRNA scaffolds containing two hairpin sequences, which recruit fluorescent...

...and Schuh labs. Clift et al. applied TRIM-away to nine proteins in ten ddifferent cell types, including...

...associated with bidirectional transcription. In an uninfected cell, dsRNA rarely circulates, but upon viral...

...subsequent cell divisions, the fraction of cells containing the particle exponentially decreases over time...

.... PubMed PMID: 15261139. Barsky, Sanford H., and Nina J. Karlin. "Myoepithelial cells: autocrine and paracrine...

... the major drawbacks of the indirect method, lengthening an often already lengthy process. (And while ...

...opposed to the opaque (and, admittedly, somewhat meaningless) practice of listing “Submitted” articles in ...

... papers is to practice reading them. There’s a learning curve for the jargon and background in your field...

...there’s a lot of control spatially as to what’s happening at different points within the wood. There are...

...Central PMCID: PMC4523203. Many empty vectors containing these fluorescent proteins are available on our...

...lines and these cells are then grown in biotin-containing media. FRET is another example of a protein fragmentation...

...receive bacteria through the mail due to laws concerning genetically modified organisms. Since Addgene...

...analyzes publications and figures using machine learning to extract important features such as influential...

...alleles, such as Cre, function when hemizygous, meaning that only one chromosome needs to contain the transgene...

...single-molecule tracking in live cells.  Controls  When designing experiments, it’s important to include proper ...

...mixture is added to the wells. Any free antibody (meaning it hasn’t bound to your protein of interest) will...

...departments across the university. Collective bargaining may provide a better deal for students in areas...

...specific antibody to the C-terminal five amino acids, meaning the tag could be quite small. However, substance...

...occurs in the few months leading up to applications opening. With virtual options, you can narrow down your...

...Twitter, for instance. If you’ve had a particularly meaningful conversation, and you have their email address...

...tags we will discuss here are all conditional, meaning that they only facilitate degradation of their ...