We narrowed to 810 results for: nin

1. Reagent Repositories Are Speeding up Science During the Pandemic

   Type

   Blog Post

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   Aug. 4, 2020, 1:15 p.m.

   ...with regards to SARS-CoV-2, this mouse strain is regaining popularity. The Jackson Laboratory, a nonprofit...

2. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

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   Blog Post

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   Sept. 19, 2017, 1:20 p.m.

   ... 2). Induced cell death was confirmed by TUNEL staining.  The video below shows what happens to the zebrafish...

3. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

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   Blog Post

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   July 27, 2017, 1:11 p.m.

   ...her science communication skills throughout her training and wants to make her work understandable to people...

4. The Power Behind NGS Plasmid Validation: seqWell

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   Blog Post

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   April 19, 2017, 3:25 p.m.

   ...are pooled together to create a single library containing fragments carrying sample-specific barcodes. ...

5. Science Career Options

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   Blog Post

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   Feb. 23, 2017, 1:30 p.m.

   ... this will give you an idea of what additional training you might need to obtain. You'll also need to ...

6. Four Factors that Differentiate the Stem Cell Field

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   Blog Post

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   Sept. 28, 2016, 2:35 p.m.

   ...but they are also the reason this research is happening. Stem cells scientists have certainly not lost...

7. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

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   Blog Post

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   March 24, 2016, 2:30 p.m.

   ...Cas9 breaks repaired by the non-homologous end-joining (NHEJ) pathway disrupt target gene sequences (thus...

8. Plasmids 101: Mammalian Vectors

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   Blog Post

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   March 25, 2014, 3:15 p.m.

   ... allow for episomal amplification of plasmids containing the viral EBV or SV40 ORIs, respectively. The...

9. Flow Cytometry Readouts: Yes, No, and Everything in Between

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   Blog Post

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   Aug. 20, 2024, 1:15 p.m.

   ...your experiment. You can see from Figure 2 that determining where to place your gate based on the dot plot...

10. Plasmids 101: Inducible Promoters

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    Blog Post

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    Jan. 18, 2018, 2:34 p.m.

    ...the activator protein can bind to the promoter, turning it ON and initiating transcription.     Negative...

11. GPCRs: How Do They Work and How Do We Study Them?

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    Blog Post

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    Feb. 11, 2021, 2:15 p.m.

    ...PRESTO-TANGO (parallel receptorome expression and screening via transcriptional output - TANGO) (Kroeze et...

12. Hot Plasmids - May 2022

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    Blog Post

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    May 26, 2022, 1:15 p.m.

    ...specific subcellular localization peptide. By combining three different localization peptides with four...

13. How Addgene Creates Content

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    Blog Post

    Published

    Nov. 17, 2022, 2:15 p.m.

    ... So how can you get involved? Keep asking your burning questions and tell us what you want to know more...

14. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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    Blog Post

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    July 28, 2015, 2:30 p.m.

    ...event. Now it’s maybe one out of 100 or 50 event. Cloning of the second plasmid can be done in one day in...

15. How to Make Friends and Meet People at a Scientific Conference

    Type

    Blog Post

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    Aug. 7, 2014, 1:58 p.m.

    ...than you – meeting scientists at your level of training is always a good idea. Make contact with other...

16. All in a Twist: dsRNA

    Type

    Blog Post

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    Feb. 2, 2023, 2:15 p.m.

    ...associated with bidirectional transcription. In an uninfected cell, dsRNA rarely circulates, but upon viral...

17. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

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    Blog Post

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    Jan. 31, 2019, 1:32 p.m.

    .... PubMed PMID: 15261139. Barsky, Sanford H., and Nina J. Karlin. "Myoepithelial cells: autocrine and paracrine...

18. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

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    Blog Post

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    April 23, 2018, 2 p.m.

    ...and Schuh labs. Clift et al. applied TRIM-away to nine proteins in ten ddifferent cell types, including...

19. Summer SciComm: Preprints

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    Blog Post

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    Aug. 16, 2022, 1:15 p.m.

    ...opposed to the opaque (and, admittedly, somewhat meaningless) practice of listing “Submitted” articles in ...

20. Antibodies 101: Secondary Antibodies

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    Blog Post

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    Dec. 21, 2021, 2:15 p.m.

    ... the major drawbacks of the indirect method, lengthening an often already lengthy process. (And while ...

21. Plasmid Tools for Microbiome Studies

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    Blog Post

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    June 27, 2019, 12:25 p.m.

    ...subsequent cell divisions, the fraction of cells containing the particle exponentially decreases over time...

22. Human Germline Editing Using CRISPR

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    Blog Post

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    Aug. 10, 2017, 2:19 p.m.

    ...and semen samples. Ma et al. designed plasmids containing Cas9 and two different gRNAs, and electroporated...

23. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

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    Blog Post

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    June 15, 2017, 2:30 p.m.

    ...an affinity of 110 µM (Zacharias et al, 2002), meaning that at this concentration 50% of the protein forms...

24. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

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    Blog Post

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    April 13, 2017, 2:30 p.m.

    ...easy to identify: double-membraned structures containing undigested cytoplasmic contents. Autophagosomes...

25. CRISPRainbow and Genome Visualization

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    Blog Post

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    Feb. 28, 2017, 3:30 p.m.

    ...CRISPRainbow paper, Ma et al. engineered gRNA scaffolds containing two hairpin sequences, which recruit fluorescent...

26. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type

    Blog Post

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    May 5, 2015, 3:54 p.m.

    ... papers is to practice reading them. There’s a learning curve for the jargon and background in your field...

27. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

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    Blog Post

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    April 22, 2015, 2:06 p.m.

    ...there’s a lot of control spatially as to what’s happening at different points within the wood. There are...

28. Early Career Researcher Toolbox: Finding Relevant Papers

    Type

    Blog Post

    Published

    Jan. 9, 2020, 1:45 p.m.

    ...analyzes publications and figures using machine learning to extract important features such as influential...

29. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type

    Blog Post

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    May 1, 2018, 1:57 p.m.

    ...lines and these cells are then grown in biotin-containing media. FRET is another example of a protein fragmentation...

30. Lab Automation at Addgene

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    Blog Post

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    March 22, 2018, 1:28 p.m.

    ...receive bacteria through the mail due to laws concerning genetically modified organisms. Since Addgene...

31. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

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    Blog Post

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    Aug. 24, 2021, 1:15 p.m.

    ...mixture is added to the wells. Any free antibody (meaning it hasn’t bound to your protein of interest) will...

32. Split Fluorescent Proteins for Studying Protein-Protein Interactions

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    Blog Post

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    May 3, 2022, 1:15 p.m.

    ...single-molecule tracking in live cells.  Controls  When designing experiments, it’s important to include proper ...

33. Mouse Modeling, Part 2: Breeding and Crossing Mice

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    Blog Post

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    Aug. 6, 2019, 12:55 p.m.

    ...alleles, such as Cre, function when hemizygous, meaning that only one chromosome needs to contain the transgene...

34. Fluorescent Proteins 101: Introduction to FRET

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    Blog Post

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    June 27, 2017, 1:03 p.m.

    ...Central PMCID: PMC4523203. Many empty vectors containing these fluorescent proteins are available on our...

35. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type

    Blog Post

    Published

    Sept. 13, 2016, 2:30 p.m.

    ...departments across the university. Collective bargaining may provide a better deal for students in areas...

36. Plasmids 101: Degron Tags

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    Blog Post

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    March 14, 2024, 1:15 p.m.

    ...tags we will discuss here are all conditional, meaning that they only facilitate degradation of their ...

37. Tips to Make the Most of a Scientific Conference

    Type

    Blog Post

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    May 23, 2019, 12:26 p.m.

    ...Twitter, for instance. If you’ve had a particularly meaningful conversation, and you have their email address...

38. Plasmids 101: Knockout/Knock-In Plasmids

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    Blog Post

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    Dec. 1, 2016, 3:30 p.m.

    ...Knockouts with CRISPR through Non-Homologous End Joining Learn How the FLEx Switch Can Be Used to Turn Genes...

39. Of Myc and Men

    Type

    Blog Post

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    Jan. 19, 2023, 2:15 p.m.

    ...specific antibody to the C-terminal five amino acids, meaning the tag could be quite small. However, substance...

40. PhD Applications After COVID

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    Blog Post

    Published

    Jan. 5, 2023, 2:15 p.m.

    ...occurs in the few months leading up to applications opening. With virtual options, you can narrow down your...

41. Google Forums Round Up: First Impressions of NgAgo

    Type

    Blog Post

    Published

    Aug. 4, 2016, 2:30 p.m.

    .... Cas9 guide RNA delivery does however rely on cloning into a vector or in vitro synthesis, which could...

42. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

    Type

    Blog Post

    Published

    April 19, 2016, 2:30 p.m.

    ...PMCID: PMC2173526. 2. Zacharias, D.A., et al., Partitioning of lipid-modified monomeric GFPs into membrane...

43. Which Fluorescent Protein Should I Use?

    Type

    Blog Post

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    May 20, 2014, 2:06 p.m.

    ...Stability: This parameter is important if you are planning to express the FP in acidic environments (e.g....

44. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type

    Blog Post

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    Sept. 6, 2018, 12:02 p.m.

    ... together to form the RNP. Detailed protocols outlining these steps have been made publicly available ...

45. Plasmids 101: Protein tags

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    Blog Post

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    Dec. 11, 2014, 4:26 p.m.

    ... be to fuse an affinity tag to it. The FLAG, hemaglutinin antigen (HA), and c-myc tags have been the workhorses...

46. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type

    Blog Post

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    Dec. 2, 2014, 7:23 p.m.

    ...necessary to isolate specific genomic regions while retaining molecular interactions. For this purpose, we developed...

47. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

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    Blog Post

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    July 7, 2020, 1:15 p.m.

    ...demonstrated that GO is an effective reporter for adenine base editors, that make A-to-G edits in the genome...

48. Supporting Reproducibility with a Connected ELN

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    Blog Post

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    Jan. 17, 2019, 1:38 p.m.

    ...possible to import Word documents into RSpace, maintaining the original formatting. In this way data is ...

49. Analyzing CRISPR Editing Results with ICE from Synthego

    Type

    Blog Post

    Published

    May 8, 2018, 1 p.m.

    ...read the paper on bioRxiv.   Using the ICE tool Learning how to use the ICE software tool is quick and ...

50. Career Insights: Technical Support Specialist

    Type

    Blog Post

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    Nov. 9, 2017, 2 p.m.

    ...comprehensive lab experience. With this valuable training behind you (and largely only that is necessary...