Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 729 results for: NSI

Showing: 161 - 170 of 729 results

  1. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ...spectral properties of the fluorophores you are considering to make sure that they’ll be compatible with ...antibody conjugates (especially if you start to consider clinical applications). But there are a few other... at least mention. Oligonucleotides allow for sensitive detection of low abundance targets with significant...mechanisms. Most take advantage of chemical groups intrinsically present on antibodies, such as the primary amine...can conjugate an antibody yourself allows you to consider a broader selection of antibodies for your experiments...

  2. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    Published
    March 15, 2022, 1:15 p.m.
    ...secondary considerations to validation, application specificity, and experimental considerations - no other...has been validated in your specific application. Consider also that there may be variations within an application... your needs.    Multiplex experiments Finally, consider whether you will be using other antibodies in ...from a vendor that you use frequently, with a responsive and knowledgeable technical support team, is ...

  3. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    Published
    Oct. 29, 2019, 2:52 p.m.
    ...channelrhodopsin used as a sensitive fluorescent voltage indicator with near-complete desensitization in continuous... the imbalance of electrical charge between the inside and the outside of the cell. This imbalance is ...Q239 - A GEVI based on the fusion of the voltage sensing domain of a Ciona intestinalis voltage sensor and...anion channel. It is triggered by a short low-intensity blue light pulse and stays open for a longer duration...

  4. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    Published
    April 13, 2017, 2:30 p.m.
    ...proteins: a relatively pH-insensitive RFP variant, DsRed.T3 and a pH-sensitive GFP variant, super ecliptic...Rosella is a pH-sensitive fluorescent biosensor that was recently deposited with Addgene by Dr. Mark ... and recycling. The key to Rosella’s autophagy-sensing abilities is that its fluorescence emission spectra...regardless of its localization in the cell. SEP’s pH sensitivity means Rosella will fluoresce green unless it’...

  5. Developing Lab Management Software for Biology

    Type
    Blog Post
    Published
    Aug. 25, 2016, 2:30 p.m.
    ... learned over the years with the hope that our insights can help steer you in the right direction when... of this is gospel, but we think it's worth consideration. Everything will go wrong Whenever an order ...easily as needed. In this case, the physical considerations of the laboratory make object composition a...technical details of the problem you're solving, but consistency is key. Some namespace collisions you may encounter...treating these pieces of data in ways that are inconsistent with how they are being treated in the lab, ...

  6. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    Published
    Feb. 11, 2014, 12:13 p.m.
    ...given problem, Empowers the writer, And offers insight into a career as a Professor. Step 1: Your ...sections in a NIH RFA, for example, and tips to consider while writing each section. Research challenge...time to review the literature and offer educated insight into what the desirable features of future technologies...deem your proposal successful. Typical proposals consist of 3 to 5 aims, with secondary milestones to meet... the application. Make sure to invest time and consider the reviewer's comments. Whether the proposal ...

  7. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    Published
    June 14, 2018, 11:44 a.m.
    ...virion is a non-enveloped particle of ~100 nm, consisting of a capsid surrounding an inner core which contains...adenovirus: the virion is ~ 100nm in size, and consists of an icosahedral capsid made of 3 types of proteins... for creating adenoviral vector constructs. It consists of 2 plasmids: a shuttle vector (in which the ...purification of rAdV uses cesium chloride (CsCl) density gradients combined with ultracentrifugation to ... rAdV genomes/mL) can be measured by: Optical density: particle concentration is measured by looking ...

  8. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 1, 2018, 1:57 p.m.
    ...Interactions Difficult to detect weak and/or transient PPIs. Many PPI identification techniques rely ...often prevents the identification of weak and/or transient interacting proteins. No spatiotemporal control...mass spec analysis. Biotinylated proteins are considered possible interaction partners if they are enriched...BioID, is also useful for identifying weak and/or transient protein-protein interactions since the biotin ...cell lysis and lysate fractionation. Important considerations for Split-BioID It’s important to remember ...

  9. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ...some reliable controls. 2. Viral stocks may be sensitive to freeze thaw cycles. Depending on the researcher...ultracentrifugation of the viral stock, followed by resuspension of the collected particles in a smaller volume...Davis et al., 2002). However, polybrene is highly sensitive to freeze-thaw cycles and should be stored in ...enhance transduction efficiency in cells that are sensitive to the toxicity of polybrene (e.g., hematopoietic...PMID: 12050007. 7. Stockschläder, M., et al. "Expansion and fibronectin-enhanced retroviral transduction...

  10. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ...III), each responsible for transcribing different types of RNA: Polymerase I is responsible for ribosomal... Rho-independent termination is also known as intrinsic termination, and it relies on the formation of...ribosomal RNA, Polymerase II is responsible for mRNA and miRNAs, and Polymerase III transcribes tRNA and other...polymerase-associated proteins, CPSF and CstF, which are responsible for recruiting the cleavage and polyadenylation...
Showing: 161 - 170 of 729 results