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...coronaviruses and enable the virus to enter host cells. To do this, they injected the spike protein from two different...effectively to protein targets, similar to how antibodies do. The structure of the common antibody is heftier ...

...guanines). This limits the use of ABEs as DNA sites that do not contain a suitable PAM cannot be edited efficiently...While base editors are precise molecular tools, they do have undesired off-target editing effects. ABEs have...

...Choosing a topic and gauging interest The first thing to do if you’ve thought about starting a journal club is...make. How often will you hold it? How many people do you expect to attend? What is the expectation on ...

...and realize you need to apply different filters you do not need to start a new search. Simply click the ...purpose (e.g. CRISPR) Beyond search What can you do if you don’t have any specific search terms in mind...

...use. This will prevent spills, but, if any spills do occur, they should be wiped with 70% ethanol immediately...morphologies, being familiar with these morphologies and doing a quick check under the light microscope will help...

...they are present at a very low concentration. So how do we make sure to catch all of these potential mishaps...expected hits, we always get hits to “random” genes. Do these hits always mean the sample is contaminated...

...Gal Haimovich of greenfluorescentblog. Be honest. Do you really know how fluorescent proteins glow?   ..., you will not be able to use far red-FPs. If you do not have a filter that will pass blue light to the...

...life. That’s convenient for researchers who want to do experiments in complex eukaryotic cells, but would...to express your protein of interest. However, you do still need a species-compatible promoter for expressing...

...five of these Cas proteins, while type I-C Cascades do not contain Cas6. Regardless of the subtype, the ...to the multi-Cas mechanism. These benefits of Cas3 do come at a cost — the previously mentioned issues ...

... team at Calico Life Sciences recently set out to do just that — by developing a new fluorescent protein... MagLOV raise broader questions like “How and why do fluorescent proteins respond to magnetic fields?”...

...components can interfere with conjugates. Make sure to do your research before conjugating at the bench.   ...to distinct, non-overlapping epitopes so that they do not interfere with each other. The detection antibody...

...cancer via insertional mutagenesis (which HIV does not do). Furthermore, molecular biologists working with ...in order to understand what these vector particles do once they come in contact with target cells (Fig....

...fluorescence microscopy (Dani, et al., 2010). How do you overcome this resolution limit? Electron Microscopy...Prakash K, et al. 2022). So what’s a researcher to do if they want to understand the ultrastructural relationships...

...time, when scientists who support open science, but do not share their own data - which seems to be the ...members or colleagues: How much unpublished AAV data do you have sitting in computers and notebooks right...

... beads are particularly advantageous because they do not require centrifugation between washes which can...controls include knockout cell lines or tissues that do not express the protein. With this control set, you...

...offer tools to help with these decisions.  So when do you want to codon optimize your gene of interest?...useful in expressing functional proteins in hosts that do not naturally express that gene. The protein you ...

...negative control to determine whether your PIPs actually do interact. Choosing the right negative control is ...scenarios that call for a simple, yes-no answer — i.e., do two proteins interact or not? — FRET is preferable...

...but orthologs like LwaCas13a do not require a specific PFS. Cas13 enzymes do not contain the RuvC and HNH...

...gradients, practice making the iodixanol layers before doing your first purification. This helped me get a feel...to learn more about different ways to titer AAV. Do you have any AAV production tips or tricks? Please...

...interference), and CRISRPa (activation) libraries that do just that (Doench et al., 2016, Sanson et al., 2018... identifying strong hits in genetic screens, they do have several limitations. CRISPRko results in a complete...