We narrowed to 468 results for: SEC

1. dTAG - You're it!

   Type

   Blog Post

   Published

   June 21, 2018, 2:06 p.m.

   ...these plasmids are available here. The Methods section of Nabet et al., Nature Chemical Biology additionally...

2. Reproducibility for Everyone: Lessons from an Open Science Collaboration

   Type

   Blog Post

   Published

   Feb. 28, 2019, 1:34 p.m.

   ...material downloads. This was particularly helpful for securing funding to support this effort. The future of ...

3. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

   Type

   Blog Post

   Published

   Jan. 31, 2019, 1:32 p.m.

   ...growth factor-β signaling, possibly inducing the secretion of growth promoting proteins, such as human growth...

4. Celebrating 15 Years of Scientific Sharing

   Type

   Blog Post

   Published

   Jan. 14, 2019, 2:08 p.m.

   ...Addgene is about half of what is standard in most sectors. The Addgenies support a high collaboration, high...

5. Summer SciComm Series: Cognitive Load

   Type

   Blog Post

   Published

   June 16, 2022, 1:15 p.m.

   ...We are now at the second post in our Summer SciComm series and it’s time for one of my favorite scicomm...

6. What's New in CRISPR - November 2021

   Type

   Blog Post

   Published

   Nov. 18, 2021, 2:15 p.m.

   ...demonstrate SEND as a modular system for packaging, secretion, and delivery of specific RNAs, such as Cre mRNA...

7. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

   Type

   Blog Post

   Published

   May 11, 2021, 1:15 p.m.

   ... used primary antibodies against mCherry, and secondary antibodies coupled to nanogold particles, that...

8. Transferable Skills Guide: Cross-team Communication

   Type

   Blog Post

   Published

   Nov. 21, 2019, 1:53 p.m.

   ...will need to write protocols, SOPs, and methods sections. The next time you write or read one of these,...

9. Human Germline Editing Using CRISPR

   Type

   Blog Post

   Published

   Aug. 10, 2017, 2:19 p.m.

   ...edited cells. The results were even lower for the second gRNA: 13.1% targeting efficiency with only 13% ...

10. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type

    Blog Post

    Published

    July 25, 2017, 1:20 p.m.

    ...of 500 nm light (turn on psRaf1) followed by 3 seconds of 400 nm light (turn off psRaf1). After this, ...

11. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type

    Blog Post

    Published

    April 13, 2017, 2:30 p.m.

    ...synthesized at the ER, delivered to the vacuole via the secretory pathway, and then cleaved to generate an active...

12. CRISPRainbow and Genome Visualization

    Type

    Blog Post

    Published

    Feb. 28, 2017, 3:30 p.m.

    ...gRNA with two different hairpins will produce a secondary color cyan, yellow, or magenta, depending on the...

13. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type

    Blog Post

    Published

    Jan. 11, 2017, 3:30 p.m.

    ...who had a contract - negotiated by their union - securing higher salaries. This decision will carry over...

14. Addgene's Tips for Plasmid Quality Control

    Type

    Blog Post

    Published

    Jan. 14, 2016, 3:30 p.m.

    ...your favorite sequencing tricks in the comments section below.  Additional Resources on the Addgene Blog...

15. Plasmids 101: Modular Cloning

    Type

    Blog Post

    Published

    March 12, 2024, 1:15 p.m.

    ...behaviors are available from Addgene and other sources; second, characterizing a part or optimizing a TU is much...

16. Progress Towards a PAM-Free CRISPR

    Type

    Blog Post

    Published

    March 7, 2024, 2:39 p.m.

    ...sequence, including propensity for off-targets, secondary structures, etc., as well as the presence of a...

17. How to Prepare for an Industry Interview

    Type

    Blog Post

    Published

    July 9, 2020, 1:15 p.m.

    ...Maybe you ask for questions after completing each section of your talk. Some video conferencing software ...

18. Open Resources and Plasmid Tools For Studying C. elegans

    Type

    Blog Post

    Published

    July 18, 2019, 12:55 p.m.

    ...PMC5693259.  Wang, Han, et al. "Split cGAL, an intersectional strategy using a split intein for refined spatiotemporal...

19. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type

    Blog Post

    Published

    July 11, 2019, 1:26 p.m.

    ...engineered mouse seems less overwhelming! In the second blog post of this series, I’ll discuss crossing...

20. A Look at Addgene's QC Process

    Type

    Blog Post

    Published

    May 6, 2025, 1:15 p.m.

    ... our collection. This standardization provides security for requestors, ensuring that the plasmid they...