...air bubbles or high concentration of fluorophores) cast shadows along the illumination path. The shadows...

... called V-K, is a CRISPR-associated transposase (CAST) that can insert large fragments of DNA. The V-F...

...RNA-Assisted Targeting) and the Zhang lab's system called CAST (CRISPR-associated Transposase) both enable gRNA-directed...

...single-percentage gel, you will have the option to cast your own gel. Handcast gels are significantly cheaper and generate...Tris-glycine 100 V, 1–2 hours Easy and cheap to handcast Short shelf-life; can alter proteins due to ...250 kDa Different sizes 4–20% gradient   Handcast or premade If you need a gradient gel, the decision...already made: gradients are extremely difficult to handcast, so unless you have an expert around, you’ll likely...hazardous chemicals, and are not always consistent. Precast gels, while expensive, reduce protocol time, can... CCD camera High initial (purchase) cost Precast gel Increased cost Save money Polyclonal... quickly Wet transfer Increased time Handcast gel Increased time; have to make as-needed ...

...Pint series. Chapter 4: The SITN Podcast, Sit'N Listen - In their podcast, the SITN team brings in scientists...The Goal of the SITN Podcast - Vini and Amy would like to develop the SITN podcast as a means for listeners...senior.  To learn more about the STIN's podcast, email sitnpodcast@gmail.com    For more info about starting...have to be crazy complicated! Listen to the full podcast for more great science communication tips or listen...of Science in the News, and member of the SITN podcast team. You can follow Vini on twitter @vinidhra....Science in the News, and is a member of the SITN podcast team. You can follow Amy on twitter @aigilson. ...the audio engineer and producer for the Addgene Podcast.     Additional Resources on the Addgene Blog ...

...Listen to our hot plasmids segment on the Addgene Podcast. Subscribe to the Addgene blog to get all the ... Listen to Our Tol2 Gateway-Compatible Toolbox Podcast Segment The impact of KLF4 N-terminal variants ...Neuroscience. 2016. PubMed PMID: 27798629 Listen to Our Podcast Segment on This New AAV Tool    mCyRFP1: Novel.... PubMed PMID: 27798609 Listen to our mCyRFP1 Podcast Segment New bright monomeric red fluorescent protein... PubMed PMID: 27869816 Listen to our mScarlet Podcast Segment    Photocleavable protein PhoCl expands...Methods. 2017. PubMed PMID: 28288123 Listen to Our Podcast Segment on PhoCl LSSmCherry1 & RDSmCherry1: Engineering...Methods. 2017. PubMed PMID: 28417998 Listen to Our Podcast Segment on Coselection with Ouabain New CRISPR ...

...science communication. Science in the News hosts a podcast, seminars, a blog, conferences, and more for non-scientists...the interview with Science in the News, Part 1 Podcast breakdown Introduction to Vini, Amy, and SITN ...Approach Different Audiences Listen to the SITN Podcast Many thanks to our guests, Vini Mani and Amy Gilson...of Science in the News, and member of the SITN podcast team.     Amy Gilson recently received her PhD ...Science in the News, and is a member of the SITN podcast team. You can follow Amy on twitter @aigilson. ...the audio engineer and producer for the Addgene Podcast.     Additional Resources on the Addgene Blog ...

...In today’s podcast, we sit down with Wei Leong Chew, a researcher at the Genome Institute of Singapore... Listen to the interview with Wei Leong Chew! Podcast breakdown Intro: Wei Leong Chew's career path and...the audio engineer and producer for the Addgene Podcast.      Additional Resources from the Addgene Blog...featured topic page Check out our Hot Plasmids Podcast Listen to our interview with synthetic biology ... addgene podcast...

... Listen here! Podcast breakdown (Time : Topic) 0:00 - 2:00 : Introduction to the podcast from producers...In this episode of the Addgene podcast, we sit down with Alex Chavez, a Clinical Fellow in Pathology ...Engineering. Alex is the first MD PhD we've had on the podcast, has worked extensively with CRISPR, and recently...the audio engineer and producer for the Addgene Podcast.     Additional Resources on the Addgene Blog ...

...interview and happy listening! Download the podcast here! Podcast breakdown (Time : Topic) 0:00 - 1:25 : Introduction...In the third installment in our podcast series, we chat with new Addgene Board Member, Michael Koeris...the audio engineer and producer for the Addgene Podcast.     Additional Resources Visit the Sample 6 website...the Addgene Blog Check the Other Posts in Our Podcast Series Get Additional Startup Advice Learn How ...